Transancestral fine mapping and functional dissection of autophagy-related SLE risk loci

自噬相关 SLE 风险位点的跨祖先精细定位和功能解剖

• 批准号: 9898320
• 负责人: Christopher J Lessard
• 金额: $ 70.12万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-09 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9898320
• 关键词: Affect; African; African American; Age; Alleles; Amino Acids; Antigen Presentation; Apoptotic; Asians; Autoimmune Diseases; Autoimmunity; Autophagocytosis; B-Lymphocytes; Bayesian Method; Binding Proteins; Bioinformatics; Biological; Biological Assay; Biological Process; CD4 Positive T Lymphocytes; CDKN1B gene; CRISPR/Cas technology; Candidate Disease Gene; Cell Cycle Progression; Cell Line; Cells; Cellular Assay; Chromatin; Chronic; Clathrin; Codon Nucleotides; Complex; Crohn' s disease; Custom; Cytoplasmic Organelle; Data; Development; Disease; Dissection; Endosomes; Etiology; European; Exhibits; Exposure to; Functional disorder; Gender; Gene Expression; Genes; Genetic; Genetic Polymorphism; Genetic Variation; Genetically Engineered Mouse; Genomics; Genotype; Haplotypes; Homeostasis; Homologous Gene; Host Defense; Human; Immune; Immune response; Immune system; Immunophenotyping; Individual; Inflammation; Koreans; Link; Linkage Disequilibrium; Lymphocyte; Lymphoid; Lysosomes; Mediating; Microbe; Minority; Molecular; Molecular Biology; Mus; Myelogenous; NADPH Oxidase; Odds Ratio; Organ; PRDM1 gene; Pathogenesis; Pathway interactions; Patient Care; Patients; Phagocytes; Play; Pluripotent Stem Cells; Population; Population Control; Process; Proteins; Public Health; Publishing; Quantitative Reverse Transcriptase PCR; RNA Splicing; Recycling; Reporting; Risk; Role; Sample Size; Signal Transduction; Sorting - Cell Movement; Spliced Genes; Stimulus; Structure; Susceptibility Gene; System; Systemic Lupus Erythematosus; T-Cell Proliferation; T-Lymphocyte; TLR7 gene; TP53 gene; Testing; Therapeutic; Thymic epithelial cell; Tissue-Specific Gene Expression; Transcript; Transport Process; Tumor Suppressor Proteins; Variant; Woman; autoinflammatory; case control; causal variant; cell type; chromatin protein; clinical care; cytokine; effective therapy; ethnic minority population; experimental study; genetic variant; genome wide association study; genomic locus; in vivo; induced pluripotent stem cell; insight; lupus-like; lysosomal proteins; member; monocyte; neutrophil; new therapeutic target; novel; novel therapeutics; pathogen; plasma cell differentiation; response; risk variant; screening; targeted treatment; tool; transcriptome sequencing

Systemic lupus erythematosus (SLE) is a chronic, heterogeneous autoimmune disease that disproportionally affects women and the disease is often more severe with end-organ damage in non-white populations. Few effective treatments exist for SLE, largely because the etiology is incompletely understood; however, the disease is likely to occur in genetically susceptible individuals upon environmental triggers. Multiple genome wide association studies (GWAS) and replication studies have identified ~100 (80 published and 20 in the publishing process) SLE susceptibility loci (P<5x10-8) using mainly European- and Asian-derived populations. Our recent Korean GWAS and other published studies of Asians have implicated a number of autophagy- related gene loci in SLE, similar to several other autoimmune diseases. Autophagy is an intracellular process that transports damage cytoplasmic organelles, macromolecular aggregates, or intracellular pathogens to the lysosome for degradation and recycling. Autophagy cross talks with the immune system and controls inflammation through effects on various immune cells to regulate immune homeostasis and to modulate host defenses. Fine mapping autophagy-related gene loci associated with SLE is necessary to gain understanding how these genetic variants affect different aspects of autophagy pathways in specific immune cell types, which may serve as a new drug target for SLE treatment. Due to linkage disequilibrium (LD), each risk locus contains multiple variants, and in some cases, multiple genes that could impact biologic functions relating to SLE pathogenesis. In Aim 1, we propose to utilize LD structure differences between Asian and African-derived ancestries to conduct transracially fine-mapping and bioinformatics analysis of seven SLE risk loci related to autophagy (ATG16L2-FCHSD2-P2RY2, PRDM1-ATG5, DRAM1, CDKN1B, CLEC16A, NCF2 and HIP1) using the Global Screening Array (GSA) that contains headroom for custom variants in SLE cases and controls of non-white minorities (4000 Asians and 3000 African Americans [AA]). By comparing haplotype structures and applying Bayesian approaches, we will obtain the best credible set of variants to pursue further. In Aim 2, we will refine credible variant list by using bioinformatics and molecular approaches to identify functional variants. This will include sorting immune cell subsets from 100 AA SLE patients and 100 AA controls (matched for age and gender) for RNA-seq and qRT-PCR to correlate allelic genotypes with differential gene expression and splicing as well as conducting molecular biology assays. The best candidates will be further tested in Aim 3 to conduct immunophenotyping, RNA-seq and autophagy functional assays to assess the consequences of gene- edited immune cell lines and induced pluripotent stem cells (iPSCs), which will determine the pertinent cell subset from SLE patients and controls to conduct autophagy functional assays for confirmation of genotypic effects. Our results will identify genetic variants underlying SLE risk loci and their molecular/cellular mechanisms leading to dysregulation of autophagy pathways contributing to the development of SLE.

系统性红斑狼疮（SLE）是一种慢性、异质性自身免疫性疾病