Spatio-temporal dynamics of genome-wide diversity of Fagus sylvatica

水青冈全基因组多样性的时空动态

• 批准号: 426505791
• 负责人: Professor Dr. Marco Thines
• 金额: --
• 依托单位: Institut für Ökologie, Evolution und Diversität
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/426505791?language=en
• 关键词: Spatio; temporal; dynamics; genome; wide

European beech (Fagus sylvatica L.) is an important forest tree species in Central Europe. However, despite extensive efforts of the scientific community little is known about the spatial and temporal genome-wide variation of this species. Even less is known regarding how this variation has helped the species to cope with climate change or what the adaptive potential may be in the face of future climate changes. These limitations are partly due to insufficient genomic resources of beech. However, recently the teams jointly submitting this proposal have published the first high quality reference genome of F. sylvatica. With the experience of this mutually beneficial collaboration, we submit the project proposal for continuing our collaborative research for synergistically combining our expertise in genomics and population genetics, working towards closing the knowledge gaps outlined above. Using the whole genome sequencing (WGS) approach, we will investigate genetic diversity and differentiation among natural populations of F. sylvatica representing the three major lineages of this species (reference populations) defined in Central Europe based on our earlier studies (based on ddRADseq). We will investigate how the neutral and non-neutral genetic diversity is transferred in a spatial, but also in a temporal manner. For this, we will study three different age classes (old trees > 200 y, saplings 40-60 y, and seedlings < 5 yrs) to trace any signatures of selection currently ongoing within the populations located in climatically different parts of the species distribution. These findings will have high importance for understanding the underlying mechanisms driving changes in neutral and adaptive genomic regions, which will be relevant for managing of genetic resources of tree species in the context of genetic conservation but also forestry practices. The research teams submitting this proposal have distinct and complementary areas of scientific excellence. The German team has long lasting interests in comparative and functional genomics, and genome evolution. The Polish team has experience on population genetics and genomics of forest trees. Joint collaboration during this project will be beneficial for both research groups, and will likely lead to long-term cooperation focused on population genomic studies.

欧洲山毛榉（Fagus sylvatica L.）是中欧重要的森林树种。然而，尽管科学界做出了广泛的努力，但对该物种的空间和时间全基因组变异知之甚少。关于这种变化如何帮助物种科普气候变化或面对未来气候变化的适应潜力，人们所知甚少。这些限制部分是由于山毛榉基因组资源不足。然而，最近联合提交该提案的团队已经公布了第一个高质量的参考基因组。森林植物凭借这种互利合作的经验，我们提交了继续合作研究的项目提案，以协同结合我们在基因组学和群体遗传学方面的专业知识，努力缩小上述知识差距。利用全基因组测序（WGS）方法，研究了野生野生居群间的遗传多样性和遗传分化。根据我们早期的研究（基于ddRADseq），在中欧定义了代表该物种（参考种群）的三个主要谱系的森林种群。我们将研究中性和非中性的遗传多样性是如何在空间上转移的，但也以时间的方式。为此，我们将研究三个不同的年龄组（老树> 200年，树苗40-60年，幼苗< 5年），以追踪位于物种分布的气候不同部分的种群中目前正在进行的选择的任何特征。这些研究结果将具有高度的重要性，了解驱动中性和适应性基因组区域的变化的潜在机制，这将是相关的遗传保护的背景下，也是林业实践的树种遗传资源的管理。提交这一提案的研究小组具有独特和互补的科学卓越领域。德国团队对比较和功能基因组学以及基因组进化有着长期的兴趣。波兰小组在林木群体遗传学和基因组学方面具有经验。该项目期间的联合合作将有利于两个研究小组，并可能导致长期合作，重点是人口基因组研究。