transmission electron microscope and small equipment for sample processing

我们在这里要求的透射电子显微镜将取代一台现有的、已有30多年历史的机器，这台机器已经由弗莱堡大学生物系成功操作。它将完全并入现有的、经DFG认证的核心设施LIC。生物学院的EM服务组目前为11个不同的研究小组处理各种各样的生物标本。这些样本的范围从比较简单的古生菌的阴性染色到试验相关的方法(相关光电子显微镜；CLEM)，这些方法研究植物细胞内真菌和细菌感染结构的细胞壁组成。很长一段时间以来，目前可用的仪器(Philipps CM-10)已经不能满足现代和具有竞争力的电子显微镜的需求。它还限制了可以处理的样本数量。此外，我们已经经历了长期的仪器故障，不再保证更换必要的部件。因此，现有的机器不能再以具有成本效益和可靠的方式进行维护。这里要求的瞬变电磁将使EM服务单位现代化，达到急需的水平。在调查了生物学院所需的样品和方法组合，并考虑了弗莱堡现有的其他机器后，我们决定申请一台类似标准的120千伏机器。这将使我们能够有效地处理几乎所有的样品。此外，我们还需要两台样品制备急需的小型机器。这些措施将确保今后的样品处理工作流程具有可持续性和成本效益。

The transmission electron microscope (TEM) that we request here will replace an existing and over 30 years old machine, which has been operated successfully by the Faculty of Biology of the University of Freiburg. It will be fully integrated into the already existing and DFG-accredited core facility LIC. The EM service unit of the Faculty of Biology currently handles a broad variety of biological specimens for eleven different research groups. These samples range from comparably simple negative stains of Archeae to piloting correlative approaches (correlative light-electron microscopy; CLEM), which address the cell wall composition of fungal and bacterial infection structures inside plant cells. It has been for a long time already that the currently available instrument (Philipps CM-10) does not match the needs for a modern and competitive electron microscopy anymore. It also limits the number of samples that can be processed. Additionally, we already experience long-lasting instrument failures and the replacement of essential pieces is no longer guaranteed. Consequently, the available machine cannot be maintained in a cost-effective and reliable manner anymore.The TEM requested here will modernize the EM-service unit to an urgently needed level. After surveying the sample and method portfolio that is required at the Faculty of Biology and considering other machines available in Freiburg, we decided to apply for a comparably standard 120 kV machine. This will enable us to process almost all samples efficiently. In addition, we request two small machines that are urgently needed for sample preparation. These will ensure a sustainable and cost-effective sample processing workflow in the future.