Analysis of interorganellar transposition of DNA

DNA 细胞间转座分析

• 批准号: DP0667006
• 负责人: Prof Jeremy Timmis
• 金额: $ 17.94万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0667006
• 关键词: Analysis; interorganellar; transposition; DNA

The movement of DNA between organelles is a major driving force in the eukaryotic evolution. In yeast about 75% of all nuclear genes may derive from protomitochondria. Though DNA transfer per se continues in all higher cells, including mammals, in most species the functional transfer of genes has stopped. It continues at a high rate in plants, giving them unique potential in evolutionary studies of the genome. We established experimentally that DNA moves frequently from the plastid (chloroplast) to the nucleus. We now aim to measure the frequency of DNA transposition from the plastid to the mitochondrion. If transposition is sufficiently frequent, the approach can be used to transformation the mitochondrial genome.

DNA在细胞器之间的运动是真核生物进化的主要驱动力。在酵母中，大约75%的核基因可能来自原线粒体。虽然DNA转移本身在所有高等细胞中继续进行，包括哺乳动物，但在大多数物种中，基因的功能转移已经停止。它在植物中继续以高速率存在，使它们在基因组的进化研究中具有独特的潜力。我们通过实验证实了DNA经常从质体（叶绿体）转移到细胞核。我们现在的目标是测量DNA从质体转座到质体的频率。如果转座足够频繁，该方法可用于转化线粒体基因组。