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低周波磁場による心筋再生促進の研究

低频磁场促进心肌再生的研究

基本信息

批准号：
18659397
负责人：
三田村好矩
金额：
$ 2.05万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Exploratory Research
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18659397/
关键词：
細胞培養生体磁気再生医療細胞・組織工学

项目摘要

低周波磁場による心筋再生促進の研究を行い,培養心筋細胞への低周波磁場曝露が,心筋細胞の機能向上を可能にするかを検討した.Wister系新生児ラット(生後2-3日目)の心臓を摘出し,ハサミで細片化した.0.1%Collagenase溶液中で1時間振培養して,心筋細胞を分散させた.細胞浮遊液をメッシュに通し,未分解組織を取り除いた後,培地DMEMを加えて約1時間培養し,線維芽細胞などの付着性細胞を取り除いた.細胞浮遊液を抽出回収し,心筋細胞のみを取り出し,心筋細胞数を調製した(5.0×105[cells/ml]).20日間培養した.ヘルムホルツコイル,信号発生器,増幅器からなる磁場曝露装置を製作した.ヘルムホルツコイルを培養器内に設置し,その中心部に培養皿を置き,心筋細胞に一様な磁場(1.5mT,50Hz正弦波)を曝露した.対照群は,別の培養器において培養した.1. 磁場曝露による培養心筋細胞の拍動能,増殖能:拍動数は顕微鏡下で目視により計数し,細胞数はMTT法により測定した.磁場曝露により高い拍動数,細胞数増加が確認された.2. 磁場効果の時間依存性:1日あたりの磁場曝露時間を1h,2h,4h,8h,24hとした複数群を用意し,培養1,3,5,7,10,15,20日目の細胞数,拍動数の経時変化を測定した.2時間磁場曝露した際に,他の曝露時間に比べ細胞数の増加が確認された.一方,拍動数は磁場曝露時間の違いによる差は見られなかった.3. 誘導電流作用の検討:同心円環状に区切った培養皿を製作し,区分ごとに同じ磁束密度に曝露されるが,異なる誘導電流が流れるようにした.磁場曝露により高い拍動数は示したが,誘導電流による差は見られなかった.誘導電流が大きくなると磁場曝露群の細胞数が対照群に比べ増加したが,ある値を境に増加率は減少した.

Research on the promotion of cardiac muscle regeneration by low-frequency magnetic fields is carried out, and the function of cardiac muscle cells is improved by exposing them to low-frequency magnetic fields when culturing cardiac cells. It is possible to remove the heart of the newborn baby (2-3 days after birth), and use the thin slices of the wister system. The heart muscle cells were dispersed and cultured in 0.1% Collagenase solution with vibration for 1 time. The cell suspension wasをメッシュに通し, undecomposed tissue をtaken り and removed いた, DMEM をadded えて and cultured for about 1 time, line dimensional sprout cells などのPay The cell suspension is extracted and recovered, the myocardial cells are extracted and extracted, and the number of myocardial cells is adjusted (5. 0×105[cells/ml]).20 day culture.ヘルムホルツコイル, signal generator, amplifier amplifier The magnetic field exposure device is manufactured and installed in the incubator, and the central part of the culture dish is installed. The cells are exposed to a magnetic field (1.5mT, 50Hz sine wave). The cells are exposed to the group, and the cells are cultured in an incubator. 1. The beating kinetic energy and growth energy of cardiac muscle cells cultured after exposure to magnetic fields: the number of beatings can be counted visually and finely under a microscopic microscope. The number of cells is measured by MTT method. The number of cells is increased by magnetic field exposure and the number of beats is increased. The increase in the number of cells is confirmed by the method. 2. Time dependence of magnetic field effect: magnetic field exposure time of 1 day, 1h, 2h, 4h, 8h, 24h, plural groups of 1 day, number of cells cultured for 1, 3, 5, 7, 10, 15, 20 days, beating The measurement of the change in the number of times. 2 The time of magnetic field exposure is the limit, and the ratio of the exposure time is the increase in the number of cells. Add the confirmation button. On the one hand, beat the number of times and the magnetic field exposure time will be exceeded. 3. Discussion of the effect of induced current: concentric ring-shaped section cutting, petri dish production, and differentiation of the same magnet The beam density is exposed, the current is induced by the different beam density, and the magnetic field is exposed. The number of beats is the same as the number of beats, and the induced current is the same as the difference. The induced current is large and the current is large. The number of cells in the magnetic field exposure group is higher than that of the illuminated group, and the increase rate of the environment is lower.