Lanthanide Doped Nanoparticles for X-ray Induced Photodynamic Therapy

用于 X 射线诱导光动力治疗的稀土掺杂纳米粒子

• 批准号: 429322689
• 负责人: Dr. Anne Nsubuga
• 金额: --
• 依托单位: Concordia University
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/429322689?language=en
• 关键词: Lanthanide; Doped; Nanoparticles; ray; Induced

The limitation of light penetration depth hampers the application of photodynamic therapy in deep-seated tumors. X-ray excited photodynamic therapy (X-PDT), which is based on X-rays activated luminescent nanoparticles, provides a novel strategy for photodynamic therapy in internal organs. The overall goal of the project is to develop a new class of nanomedicines consisting of radioluminescent nanoparticles coupled with light-activated drugs known as photosensitizers. In the absence of radiation, the individual components are nominally non-toxic, making this a radiation-gated intervention that is only active in the target volume. The proposed research consists of primary two pursuits: (i) optimization of nanomedicine formulation and (ii) the application of the optimized formulation to a panel of bladder cell lines to evaluate the potential of X-PDT for clinical chemo-radiotherapy. The focus will be on the nanosystem consisting of hexagonal phase ß-Na(Lu,Gd)F4: Tb3+ nanocrystals combined with rose Bengal as the photosensitizer. Lanthanide doped nanoparticles are of great interest, owing to their narrow emission bands, long-lived excited states, large anti-Stokes shifts, high resistance to photobleaching, and low toxicity. Lanthanide ions as dopants are excellent for scintillation materials, as they have high atomic number and suitable electronic energy states to emit photons in the UV, visible, and near-infrared region of the electromagnetic spectrum. These nanoconjugates with a high colloidal stability and biocompatibility can generate a reasonable amount of cytotoxic singlet oxygen (1O2) through efficient energy transfer upon external X-ray radiation, which enables them to be potentially applied in diagnosis and photodynamic therapy for deep seated tumor.

光穿透深度的限制阻碍了光动力治疗在深部肿瘤中的应用。x射线激发光动力疗法（X-PDT）是一种基于x射线激活的发光纳米粒子的光动力疗法，为内脏器官的光动力治疗提供了一种新的策略。该项目的总体目标是开发一种新型纳米药物，该药物由放射发光纳米颗粒与称为光敏剂的光活化药物结合而成。在没有辐射的情况下，单个成分名义上是无毒的，这使其成为一种辐射门控干预，仅在目标体积中有效。该研究主要包括两个方面：(i)纳米药物配方的优化；（ii）将优化后的配方应用于膀胱细胞系，以评估X-PDT在临床化疗中的潜力。重点研究了六方相的β - na (Lu,Gd)F4: Tb3+纳米晶体以孟加拉玫瑰为光敏剂组成的纳米体系。镧系掺杂纳米粒子由于其窄发射带、长激发态、大的反斯托克斯位移、高抗光漂白和低毒性而引起了人们的极大兴趣。镧系离子作为掺杂剂是极好的闪烁材料，因为它们具有高原子序数和合适的电子能态，可以在电磁波谱的紫外、可见光和近红外区域发射光子。这些纳米偶联物具有较高的胶体稳定性和生物相容性，可以在x射线照射下通过有效的能量转移产生一定量的细胞毒性单线态氧（1O2），这使得它们在深部肿瘤的诊断和光动力治疗中具有潜在的应用前景。