Development of High Quarity of Chemical Sensors Based on Polymeric Thin Film Coating

基于聚合物薄膜涂层的高质量化学传感器的开发

基本信息

批准号：
01470064
负责人：
OYAMA Noboru
金额：
$ 3.52万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

Polymer coated electrodes (PCEs), which consist of a metallic or carbon conductor direct coated with an ion-sensitive, electrocatalytic, or enzyme membrane, are very attractive potentiometric and amperometric sensors due to their simplicity of operation, superior amperometric response characteristics, capability of minimization, etc., compared with conventional membrane electrodes.In this research, new amperometric sensors for determination of glucose and cholesterol based on bilayer film coating have been fabricated and their sensor characteristics under a steady-state condition have been examined. The glucose or cholesterol concentration could be monitored by measuring the current for H_2O_2 or O_2 reduction electrocatalyzed inner film where H_2O_2 was produced orO_2. was consumed by the enzyme reaction in the outer enzyme films. The reproducible relationship between glucose or cholesterol concentration and sensor output was obtained. The PCEs functioned as an electrochemical detecto … More r for flow injection analysis. Substantial unprovement in the selectivity of arnperometric detection for flow injection system was observed as a result of an electrostatic interaction between the positively charged electropolymerized film and analysis, that is, cationic species (e. g., dopamine, 1-norepinephrine and DL-3, 4-dihydroxyphenylalanine) exhibited little response, while the response of neutral and anionic species (e. g., 3, 4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and catechol) was only slightly changed at the polymer coated electrode compared with the bare electrode.The complex admittance measurements of the quartz crystal oscillators with and without a film coating, which are in contact with air, moisture and supporting electrolyte aqueous media of various concentrations have been carried out over a frequency range covering the area of the resonant frequency of each oscillator system, and the results have been represented in the form of conductance and susceptance spectra (i. e., conductance vs. frequency plots and susceptance vs. frequency plots). According to the electrical equivalent circuit model conventionally proposed for a piezoelectric quartz crystal, the related circuit parameters (resistance R, inductance L, capacitance C and static capacitance C_0) have been evaluated, especially as a function of the concentration of supporting electrolyte. The change of electrolyte concentration-dependent swelling of the film was characterized by the evaluated parameters. It is demostrated that the method described can can apply the characterized kinematic viscosity and affinity sensing of the organic, inorganic and biological thin films. The quartz crystal microbalance (QCM) when operated in liquids was shown to retain its high mass sensitivity. The QCM has therefore been applied to the mass changes occurring during redox in thin polymer films. Solvent transport during electroneutrality requiring ion transport has been qualitatively demonstrated in the case of electrodes modified with thin film of poly (vinyferrocene), polyaniline, etc. Less

聚合物涂层电子（PCE）由金属或碳导体组成，直接覆盖，其对离子敏感的，电催化或酶膜的膜是非常有吸引力的电位和临床传感器，并且由于其操作的简单性，具有较小的安培响应特征，与常规型杂音的能力相比，它们具有较小的临床响应特征，因此它们具有较小的临床响应特征。基于双层膜涂层的葡萄糖和胆固醇已经制造出来，并且已经检查了其在稳态条件下的传感器特性。可以通过测量H_2O_2或O_2还原电催化的内膜的电流来监测葡萄糖或胆固醇浓度，其中产生了H_2O_2。被外酶膜中的酶反应消耗。获得了葡萄糖或胆固醇浓度与传感器输出之间的可再现关系。 PCE充当电化学检测器…更多用于流动注射分析的R。观察到正面注射系统的弧度检测选择性的实质性不存在，这是由于正负电荷电聚合膜和分析之间的静电相互作用而观察到的，即阳离子物种（例如多巴胺，1-近去肾上腺素和DL-3，DL-3，4-二氢二甲基）的响应，而不是中性响应的响应（例如3，3，4-二羟基乙酸，4-羟基-3-羟基-3-甲氧基乙酸和catechol）在聚合物涂层电极上与裸电的电极相比，在聚合物涂层的电极处仅略有变化。复杂的录音剂的复杂录像测量值，具有多种型号的石英晶体振荡器，并且与膜的均具有膜的覆盖率，并且与空中覆盖，与空中造型相比，与空中造成的相互作用，与空中的相互作用，与空中造成的相互作用，与空中造成的相互作用，与空中造型相比在涵盖每个振荡器系统谐振频率区域的频率范围内进行，结果以电导和感知光谱的形式表示（即电导与频率图与频率图与频率图）的形式。根据对压电石英晶体的常规电路模型，已经评估了相关电路参数（电阻R，电感L，电容C和静态电容C_0），尤其是作为支持电解质浓度的函数。膜的电解质浓度依赖性肿胀的变化以评估参数为特征。可以说明所描述的方法可以应用有机，无机和生物薄膜的特征运动粘度和亲和力灵敏度。在液体中操作时，石英晶体微量平衡（QCM）被证明保持其高质量灵敏度。因此，QCM已应用于薄聚合物膜中氧化还原期间发生的质量变化。在用薄膜（vinyferrocene），聚苯胺等修饰的电极中，在质量上证明了需要离子传输的溶剂转运。