Development of Micro-multichannel Sensors Based on Quartz Crystal Microbalance and Electrochemiluminescence

基于石英晶体微天平和电化学发光的微型多通道传感器的研制

基本信息

批准号：
11555228
负责人：
OYAMA Noboru
金额：
$ 8.77万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The subject of this research supported by Grant-in-Aid for Scientific Research (B)(2) is to develop highly sensitive multichannel quartz crystal microbalance sensors in combination with electrochemiluminescence.Principles and methods for scanning electrode quartz crystal analysis were established, in which a microelectrode was scanned over a quartz crystal, the opposite side of which was coated with a normal electrode. It was found that qualitative mapping of the mass distribution as well as distributions of viscoelastic changes in surface layers on the quartz substrate was possible in the lateral resolution of 1mm for a 9MHz quartz crystal.Further development based on the scanning electrode quartz crystal microbalance was carried out to fabricate a multichannel quartz crystal microbalance (MQCM), by arranging arrays of quartz crystal resonators on a single quartz wafer. Several types of MQCM with 4-channels were made and characterized. As a result, it is confirmed that there were no interference among resonators on an MQCM and thus each resonator is possible to function as an independent detection device. Simultaneous measurements of multi-component were successfully conducted using MQCM.Development of highly emissive electrochemiluminescence (ECL) systems was another subject of this research. Effects of parameters such as TPA concentration, pH, etc., were examined for an ECL system based on Ru(bpy)3^<2+> and tripropylamine (TPA). It was found that addition of NaN3 enhanced the intensity of the luminescence from the complex. Furthermore, surfactants also affected the ECL responses of the Ru(bpy)3^<2+>-TPA system. ECL responses of a Ru complex derivative immobilized on a magnetic microbead surface was also studied. The intensity of ECL from magnetic microbeads was strongly dependent on aggregation of the microbeads.

这项由授予科学研究支持的这项研究的主题（b）（2）是要开发高度敏感的多通道石英晶体微量平衡传感器与电化学发光的结合。扫描电极石英晶体分析的原理和方法是在该电极晶体中建立了一个平方侧面的Quartz cop and for a per a per a farectal co and a per and farectal co conal co and per a per and fare coan for a per a per and fare con a per a per and fare。发现发现在1mm的横向分辨率中，对于9MHz石英晶体的横向分辨率，可以根据扫描电极的扫描电极石英晶体晶体晶体晶体晶体晶体晶体晶体晶体晶体晶体微微亲量（均基于水晶晶体微微生物）（ed y Microbobalance（Microbalane cym cyrannel cyrannel cyrane crystrance）（均可制造，质量晶体）在1mm的横向分辨率中，可以在1mm的侧面分辨率（1mm）的横向分辨率中进行质量分布以及粘弹性变化的定性映射。单个石英晶片上的晶体谐振器。制作并表征了几种具有4通道的MQCM。结果，可以证实，MQCM上的谐振器之间没有干扰，因此每个谐振器都可以用作独立的检测装置。使用MQCM成功地进行了多组分的同时测量。高度发射电化学发光（ECL）系统的开发是这项研究的另一个主题。检查了基于RU（BPY）3^<2+>和Tripropymine（TPA）的ECL系统的参数（例如TPA浓度，pH等）的影响。发现NAN3的添加增强了复合物的发光强度。此外，表面活性剂还影响了RU（BPY）3^<2+> -TPA系统的ECL响应。还研究了固定在磁微粒表面上的RU复合衍生物的ECL响应。来自磁性微粒的ECL强度很大程度上取决于微粒的聚集。