Development of Micro-multichannel Sensors Based on Quartz Crystal Microbalance and Electrochemiluminescence

基于石英晶体微天平和电化学发光的微型多通道传感器的研制

• 批准号: 11555228
• 负责人: OYAMA Noboru
• 金额: $ 8.77万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11555228/
• 关键词: quartz crystal microbalance; electrochemiluminescence; multichannel sensors; magnetic microbeads; highly sensitive biosensors; immunoassay; clinical medicine; 臨床医学; マルチアレー

The subject of this research supported by Grant-in-Aid for Scientific Research (B)(2) is to develop highly sensitive multichannel quartz crystal microbalance sensors in combination with electrochemiluminescence.Principles and methods for scanning electrode quartz crystal analysis were established, in which a microelectrode was scanned over a quartz crystal, the opposite side of which was coated with a normal electrode. It was found that qualitative mapping of the mass distribution as well as distributions of viscoelastic changes in surface layers on the quartz substrate was possible in the lateral resolution of 1mm for a 9MHz quartz crystal.Further development based on the scanning electrode quartz crystal microbalance was carried out to fabricate a multichannel quartz crystal microbalance (MQCM), by arranging arrays of quartz crystal resonators on a single quartz wafer. Several types of MQCM with 4-channels were made and characterized. As a result, it is confirmed that there were no interference among resonators on an MQCM and thus each resonator is possible to function as an independent detection device. Simultaneous measurements of multi-component were successfully conducted using MQCM.Development of highly emissive electrochemiluminescence (ECL) systems was another subject of this research. Effects of parameters such as TPA concentration, pH, etc., were examined for an ECL system based on Ru(bpy)3^<2+> and tripropylamine (TPA). It was found that addition of NaN3 enhanced the intensity of the luminescence from the complex. Furthermore, surfactants also affected the ECL responses of the Ru(bpy)3^<2+>-TPA system. ECL responses of a Ru complex derivative immobilized on a magnetic microbead surface was also studied. The intensity of ECL from magnetic microbeads was strongly dependent on aggregation of the microbeads.

本课题是在国家科学研究资助项目（B）（2）的资助下，以电化学发光为基础，研制高灵敏度的多通道石英晶体微天平传感器，建立了扫描电极石英晶体分析的原理和方法。结果表明，在9 MHz石英晶体的横向分辨率为1 mm的条件下，可以定性地绘制出石英基片表面层的质量分布和粘弹性变化分布，并在扫描电极石英晶体微天平的基础上进一步研制了多通道石英晶体微天平（MQCM），通过在单个石英晶片上布置石英晶体谐振器的阵列。制作了几种四通道多通道微通道显微镜，并对其进行了表征。结果，确认了MQCM上的谐振器之间没有干扰，因此每个谐振器可以用作独立的检测装置。本研究的另一个主题是开发高发射率的电化学发光（ECL）体系。TPA浓度、pH等参数的影响，研究了Ru（bpy）3^<2+>和三丙胺（TPA）的电化学发光体系。结果发现，NaN 3的加入增强了配合物的发光强度。此外，表面活性剂对Ru（bpy）3^<2+>-TPA体系的电化学发光也有影响.研究了钌配合物衍生物固定在磁性微球表面的电化学发光响应。磁性微球的电致发光强度强烈依赖于微球的聚集。

项目成果

Noboru Oyama: "Electrochemiluminescence Reactions of Metal Complexes Immobilized on Surface of a Magnetic Microbead"Studies in Surface Science and Catalysis. 132. 943-946 (2001)

Noboru Oyama：“固定在磁微珠表面的金属配合物的电化学发光反应”表面科学和催化研究。

Yuzaburo Namba: "Highly Sensitive Electrochemilumi-nescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads"Analytical Sciences. 15. 1087-1093 (1999)

Yuzaburo Namba：“使用结合在磁微珠上的钌螯合物标记抗体进行高灵敏电化学发光免疫分析”分析科学。

Tetsu Tatsuma: "Electrochemically Induced Mass Transfer and Rheological Changes of Nafion Coatings Fully Loaded with [Os (bpy)_3^<2+>]"J.Electroanal.Chem.. 469. 34-42 (1999)

Tetsu Tatsuma：“满载 [Os (bpy)_3^<2 >] 的 Nafion 涂层的电化学诱导传质和流变变化”J.Electroanal.Chem.. 469. 34-42 (1999)

T.Tastuma, K.Mori and N.Oyama: "Scanning Electrode Quartz Crystal Analysis. Back-Scanning Mode and Application to Electrochemical Measurements"Analytical Sciences. 15. 749-753 (1999)

T.Tastuma、K.Mori 和 N.Oyama：“扫描电极石英晶体分析。反向扫描模式及其在电化学测量中的应用”分析科学。

T.Tatsuma, M.Ozaki and N.Oyama: "Electrochemically Induced Mass Transfer and Rheological Changes of Nafion Coatings Fully Loaded with [Os(bpy)3^<2+>]"J.Electroanal.Chem. 469. 34-42 (1999)

T.Tatsuma、M.Ozaki 和 N.Oyama：“满载 [Os(bpy)3^<2 >] 的 Nafion 涂层的电化学诱导传质和流变变化”J.Electroanal.Chem。