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Molecular and Biochemical Studies on Copper Resistance of Plant Pathogenic Bacteria

植物病原菌铜抗性的分子与生化研究

基本信息

批准号：
01480051
负责人：
GOTO Masao
金额：
$ 4.35万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480051/
关键词：
Plant pathogenic bacteria copper resistance plasmids copper compounds Bacterial canker of kiwifruit Bacterial grain rot of rice Kocide Copper sulfate プラスミド分子生物学生化学

项目摘要

Copper resistance of plant pathogenic bacteria (5 genus, 31 species and 179 strains) was examined with copper sulfate and copper hydroxide (Kocide). The bacteria were exposed to these copper compounds in the from of agar plate (Bacto potato-dextrose agar) and of aqueous solution. The minimum inhibitory concentration (MIC) of copper sulfate obtained by the agar method was one hundred times higher than that by aqueous solution. The high resistance to copper sulfate was observed with P. caryophylli (3mM), P. cepacia (4.5 mM), P. gladioli (4.25 mM), P. glumae (3.2 mM), P. solanacearum (2.25 mM), P. sytingae pv. aceris (2.25 mM), P. syringae pv. actinidiae (3.0 mM), P. syringae pv. maclicola (2.0 mM), P. syringae pv. tomato (2.75 mM), P. tolaasii (2.75 mM), Erwinia chrysanthemi pv. zeae (2.75 mM), Agrobacterium tumefaciens (2.25 mM), and Xanthomonas capmpestris pv. vesicatoria (1.75 mM). The members of rRNA group II of Pseudomonas were consistent in the high copper resistance. Each bacteriu … More m was???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????Copper resistance in P. glumae, P. syringae pv. actinidiae, P. syringae pv. maculicola and P. syringae pv. tomato was confirmed to be plasmid origin. The plasmid of P. glumae (pPgCul) and P. syringae pv. actinidiae (pPaCul) were self-transmissible. Another plasmid of the latter (pPaCu2) was not transmissible. Copper-resistance plasmids did not always associate with high level of resistance. In P. sytingae pv. photiniae, for example, moderate copper resistance (MIC 0.8 mM) was also determined by a plasmid, although its properties have not been elucidated in detail. These copper-resistance plasmids sometimes carried other phenotypic properties e. g., streptomycin resistance in plasmids of P. syringae pv. actinidiae and extracellular polysaccharide synthesis in the plasmid of P. syringae pv. maculicola.In the majority of bacteria tested, high correlation was observed between the degree of resistance to copper sulfate and that to copper hydroxide. However, P. cepacia, P. gladioli, P. glumae and P. sytingae pv. actinidiae were different from others in the behavior to these two copper compounds. In P. syringae pv. actinidiae, the resistance to copper hydroxide was greater than that to copper sulfate. The relationship was just opposite in P. cepacia, P. gladioli and P. glumae. These facts suggested that the diverse mechanisms are involved in copper resistance of plant pathogenic bacteria.Even in bacteria with the highest MIC to copper sulfate, the MIC to copper hydroxide or Kocide was still within the range of concentrations for practical spray. Thus, the resistance to copper copmpounds in plant pathogenic bacteria may not necessarily imply the ineffectiveness of these compounds in control of bacterial plant diseases. Less

用硫酸铜和氢氧化铜（Kocide）检测植物病原菌（5属、31种、179株）的铜抗性。将细菌暴露于琼脂平板（Bacto 马铃薯葡萄糖琼脂）和水溶液中的这些铜化合物。琼脂法得到的硫酸铜的最低抑菌浓度（MIC）比水溶液法高出一百倍。观察到石竹假单胞菌 (3mM)、洋葱假单胞菌 (4.5 mM)、唐菖蒲假单胞菌 (4.25 mM)、颖壳假单胞菌 (3.2 mM)、青枯假单胞菌 (2.25 mM)、丁香假单胞菌 (2.25 mM) 和 P. sytingae pv. 对硫酸铜的高抗性。 aceris (2.25 mM)、P. syringae pv.猕猴桃 (3.0 mM)、丁香假单胞菌 pv. maclicola (2.0 mM)、丁香假单胞菌 pv.番茄 (2.75 mM)、P. tolaasii (2.75 mM)、菊欧文氏菌 pv.玉米 (2.75 mM)、根癌农杆菌 (2.25 mM) 和黄单胞菌 pv.囊泡 (1.75 mM)。假单胞菌rRNA组II的成员在高铜抗性方面是一致的。每个细菌……更多米曾是？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？铜 P. glumae、P. syringae pv. 的抗性猕猴桃，丁香假单胞菌 pv。 maculicola 和 P. syringae pv.番茄被证实是质粒来源。 P. glumae (pPgCul) 和 P. syringae pv. 的质粒。猕猴桃 (pPaCul) 具有自我传播性。后者的另一个质粒(pPaCu2)是不可传播的。铜抗性质粒并不总是与高水平的抗性相关。在 P. sytingae pv。例如，石楠也具有中等的铜抗性（MIC 0.8 mM），但其特性尚未详细阐明。这些铜抗性质粒有时携带其他表型特性，例如。例如，丁香假单胞菌质粒中的链霉素抗性。紫丁香假单胞菌质粒中的猕猴桃和胞外多糖合成。在大多数测试的细菌中，观察到对硫酸铜和氢氧化铜的耐药程度之间存在高度相关性。然而，P.cepacia、P.gladioli、P.glumae 和 P.sytingae pv。猕猴桃对这两种铜化合物的行为与其他猕猴桃不同。丁香假单胞菌 pv。猕猴桃对氢氧化铜的抵抗力大于对硫酸铜的抵抗力。在洋葱 P. cepacia、剑兰 P. glumae 和 P. glumae 中，这种关系正好相反。这些事实表明，植物病原菌的铜抗性涉及多种机制。即使在对硫酸铜具有最高 MIC 的细菌中，对氢氧化铜或 Kocide 的 MIC 仍然在实际喷雾的浓度范围内。因此，植物病原细菌对铜化合物的抗性不一定意味着这些化合物在控制细菌性植物病害方面无效。较少的