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Structure and Function of Phosphonosphingoglycolipids Containing Pyruvylated Galactose.

含有丙酮酰化半乳糖的磷酸鞘氨醇糖脂的结构和功能。

基本信息

批准号：
01480144
负责人：
SATAKE Mei
金额：
$ 4.35万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

1. Structures of FGL-IIa and FGL-V : Our previous studies revealed that one of the nervous tissuespecific monophosphonoglycosphingolipid FGL-IIb of Aplysia kurodai contains pyruvylated galactose at its non-reducing end and L-fucose as a branched sugar. Anti-FGL-IIb antiserum reacts immunologically with many other phosphonoglycolipids including FGL-IIa and FGL-V. By GC-MS, NMR and chemical methods, the complete structures of FGL-IIa and FGL-V were determined as follows :Thus three pyruvic acid containing phosphonoglycosphingolipids have been shown to have the back bone structure common to all other phosphonoglycosphingolipids isolated from Aplysia, GalNAcalphal->3Galbetal->4Glcbetal->1Ceramide. Pyruvic acid is linked to terminal galactose through 3 and 4 hydroxyl by sconfiguration.2. Cellular and subcellular distribution of pyruvic acid containing phosphonosphingoglycolipids : Antiserum raised against to FGL-IIb in the rabbit stained only nerve bundles in the tissues of Aplysia. No neuronal cell bodies were stained. Immuno-light microscopically, axonal membranes were not stained but small round cells surrounding the axons or intercellular elements seemed to be stained. Immuno-electron microscopic examination has being carried out.3. Studies on biological activities of pyruvate containing phosphonosphingoglycolipids : Their peculiarities in the chemical structures and the tissue distribution imply their neurobiological functions in axonal growth or axonal fasciculation. Three lines of experiment have been carried out. Effects of the lipids or their antisera on axonal growth of isolated Aplysia neuron, on fiber outgrowth from clonal cells and on axonal outgrowth from cut-end of the commissural nerve of Aplysia. Anti FGL-IIb antiserum showed a little effects in the last experiment.

1. FGL-IIa和FGL-V的结构：我们先前的研究发现黑黑的一种神经组织特异性单磷酸甘磷脂FGL-IIb在其非还原端含有丙酮化半乳糖，L-位点作为支链糖。抗fgl - iib抗血清可与FGL-IIa和FGL-V等多种磷脂发生免疫反应。通过气相色谱-质谱、核磁共振和化学方法，确定了FGL-IIa和FGL-V的完整结构如下：由此可见，三种含磷酸甘氨脂的丙酮酸具有从海葡属植物中分离的其他磷酸甘氨脂所共有的背骨结构：GalNAcalphal- bbb3galbetal - bbb4glbetal - >ceramide。丙酮酸通过3和4个羟基与末端半乳糖相连。含磷酸磷脂糖脂的丙酮酸在家兔组织中细胞和亚细胞分布：FGL-IIb抗血清在家兔组织中仅染色的神经束。未见神经元细胞体染色。免疫光镜下，轴突膜未被染色，但轴突周围的小圆形细胞或细胞间元件似乎被染色。已进行免疫电镜检查。含膦鞘糖脂的丙酮酸酯的生物学活性研究：其化学结构和组织分布的特殊性暗示了其在轴突生长或轴突束化中的神经生物学功能。进行了三组试验。脂质及其抗血清对海兔离体神经元轴突生长、克隆细胞纤维生长和交联神经切端轴突生长的影响。在最后的实验中，抗FGL-IIb抗血清效果不明显。