The changes of spinal blood flow and neurotransmitters after experimental spinal cord injury in rabbits.

家兔实验性脊髓损伤后脊髓血流和神经递质的变化。

• 批准号: 01480372
• 负责人: SAKOU Takashi
• 金额: $ 4.1万
• 依托单位: Kagosima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480372/
• 关键词: Experimental acute spinal cord injury; Spinal blood flow; Spinal edema; Monoamine; Excitatory amino acid; 脊髄内血管透過性; レ-ザ-ドップラ-血流計; レ-ゲ-ドップラ-血流計

The changes in spinal micro circulation and the effects of endogenous excitatory amino acid on the pathogenic mechanism of paralysis after acute spinal cord injury were system have been recognized in recent years. In this study, various factors concerning microcirculation and monoamines levels were compared between rabbits in which excitatory amino acid receptor (NMDA) antagonist MK801 administerd (MK801-treated group) and untreated controls (control group) after spinal cord injury.The following studies were performed.1)Evaluation of motor paralysis according to the Talov's evaluation.2)Determination of the spinal blood flow by laser doppler flowmetry.3)Quantitative determination of spinal edema by the dry weight method.4)Microdetermination of fluorescent materials (FD 70S) to detect changes in intraspinal vascular permeability.5)Determination of intraspinal monoamines and their metabolites by HPLC with electrochemical detection.Results1)Motor paralysis improved significantly in the MK801-treated group after 24 hours.2)Spinal blood flow was decreased after trauma substantially, but there was no signficant difference between the two groups during 6 hours after injury.3)Severity of the spinal edema in the MK801-treated group was significantly less than that in the control group, 1 hour and 6 hours after injury.4)An increase in intraspinal vascular permeability in the MK801-treated group was significantly less than that in the control group, one hour after injury.5)MHPG/NE ratio was significantly greater in the MK801-treated group than in the control group.The present study revealed that NMDA antagonist MK801 improved behavioral outcome, spinal edema, and vascular permeability after acute spinal cord injury. Therefore it was strongly suggested that NMDA plays an important role in the secondary spinal cord damage.

近年来，脊髓微循环的改变及内源性兴奋性氨基酸在急性脊髓损伤后瘫痪发病机制中的作用已被系统认识。在本研究中，比较了兴奋性氨基酸受体（NMDA）拮抗剂MK 801对家兔微循环的影响及单胺类神经递质的变化。（MK 801给药组）和未给药对照组进行以下研究：1）根据Talov的评估，评估运动麻痹。2）用激光多普勒血流仪测定脊髓血流量。3）用干重法定量测定脊髓水肿。4）荧光物质（FD 70 S）的微量测定，以检测椎管内血管通透性的变化。5）结果1）MK 801治疗组伤后24 h运动麻痹明显改善; 2）脊髓血流量明显减少，伤后24 h脊髓血流量减少。但伤后6小时内两组间无显著性差异。3）MK 801治疗组脊髓水肿程度明显轻于对照组，4）MK 801治疗组脊髓内血管通透性的增加明显小于对照组，5）MK 801治疗组的MHPG/NE比值明显高于对照组。本研究表明，NMDA拮抗剂MK 801可改善急性脊髓损伤后的行为学结果、脊髓水肿和血管通透性。提示NMDA在继发性脊髓损伤中起重要作用。

项目成果

Takashi Sakou: "The changes of spinal blood flow and neurotransmitters after experimental spinal cord injury in rabbits" Medicine Philosophica.

Takashi Sakou：“兔子实验性脊髓损伤后脊髓血流和神经递质的变化”医学哲学。

Takashi Sakou: "Role of N-methyl-D-aspartate (NMDA) excitatory amino acid receptor in microcirculation after experimental acute spinal cord injury" The Central Japan of Orthopaedic Surgery and Traumatology. 33 No. 5. 2015-2017 (1990)

Takashi Sakou：“N-甲基-D-天冬氨酸（NMDA）兴奋性氨基酸受体在实验性急性脊髓损伤后微循环中的作用”日本中部骨科外科和创伤学。

酒匂 崇: "実験的脊髄損傷における興奮性アミノ酸の役割" 中部日本整形外科災害外科学会雑誌. 33. 2015-2017 (1990)

Takashi Sakawa：“兴奋性氨基酸在实验性脊髓损伤中的作用”日本中央整形外科学会杂志 33. 2015-2017 (1990)。

Takashi Sakou: "Effect of Excitatory Amino Acid to Monoaminergic Responses in Spinal Cord Injury" The Journal of the Japanese Orthopaedic Association. 64 No. 8. S1109 (1990)

Takashi Sakou：“兴奋性氨基酸对脊髓损伤中单胺能反应的影响”日本骨科协会杂志。

Takashi Sakou: "Role of Excitatory Amino Acid Receptor(NMDA) in Experimental Acute Spinal Cord Injury" Combined Meeting of.the Orthopaedic Research Societies of U.S.A.,Japan and Canada. 322 (1991)

Takashi Sakou：“兴奋性氨基酸受体（NMDA）在实验性急性脊髓损伤中的作用”美国、日本和加拿大骨科研究会联合会议。