Ultrastructural and immunohistochemical studies on the development of the tooth germ cultured by the organ culture system.

器官培养系统培养的牙胚发育的超微结构和免疫组织化学研究。

基本信息

批准号：
01480424
负责人：
TERASHIMA Tatsuo
金额：
$ 4.42万
依托单位：
TOKYO MEDICAL AND DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

We performed ultrastructural and immunohistochemical studies on the development of the mouse lower first molar tooth germ that cultured by organ culture system. (1) Ultrastructural observation. : On culture day 4, undifferentiated cells of dental papilla were differentiated to the odontoblasts in the cusp area followed the formation of the dentin matrix. On culture day 8, the initial calcification in dentin matrix was observed, and the ameloblasts began to secrete enamel matrix with initial deposition of enamel crystals in the area adjacent to the calcified dentin. On culture day 16, the ameloblasts were shortened and two types of ameloblasts( ruffle ended and smooth ended ameloblast ) with the wide intercellular space were observed. But, the formation of dentin were ceased by the degeneration of the odontoblast in the area adjacent to the highly calcified enamel. On culture day 24, the maturation of enamel were proceeded, and the crown formation of molar was completed. These observati … More ons suggested that the development of tooth germs in organ culture system were similar to those of in vivo except for the delay of the time required to the same developmental stage. (2) Immunohistochemical observation. : The gold label that indicated to the immunoelectronscopic localization of the enamel protein was initially observed to the stippled material in the distal end of the ameloblasts located to the area that the basal lamina was discontinuous by the collapse of basal lamina. In the ameloblasts of secretory stage, the gold labels were observed to vesicles and vacuoles in the distal portion and the golgi area of the ameloblasts, but not observed to the cisternae in the rough endoplasmic reticulum. In the ameloblasts of the maturation stage, the gold labels were located in the intermembramous space, vesicles and vacuoles in the area of the ruffle border. These observations suggested that the localization of the enamel protein of the tooth germs in the organ culture system were similar to those of in vivo. This organ culture system is useful for study to the mechanism of the enamel formation. (3) We obtained the ratmouse chimea monoclonal antibodies to the mouse enamel protein. The enamel and the distal portion of the ameloblasts of the mouse were specifically immunostained. Less

我们对由器官培养系统培养的小鼠下部第一摩尔牙齿胚芽的发展进行了超微结构和免疫组织化学研究。（1）超微结构观察。：在培养第4天，牙齿乳头状的未分化细胞被与牙蛋白乳突区域的odontoblasts区分开，随着牙本质基质的形成。在培养第8天，观察到牙本质基质中的初始计算，并且成成木开始分泌牙釉质基质，并在与钙化牙本质相邻的区域中初始沉积搪瓷晶体的初始沉积。在培养第16天，缩短了木材细胞，并观察到两种类型的成成木材（荷叶边结束，末端的木质细胞末期平滑），并观察到宽的细胞间空间。但是，通过与高度钙化的牙釉质相邻区域中的牙糖细胞的变性，牙本质的形成停止了。在第24天培养，进行了搪瓷的成熟，并完成了摩尔的冠状形成。 Observati…更多的ONS提出，器官培养系统中牙齿的发育与体内的牙齿细菌相似，除了延迟了同一发育阶段所需的时间。（2）免疫组织化学观察。：最初观察到牙釉质蛋白的免疫电子定位的金标签，最初是在基底层塌陷到位于基础层层不连续的面积的木质细胞远端的固定物质上观察到的。在分泌阶段的成成木细胞中，在远端部分和阿不成细胞的高尔夫区域观察到金标签，但在粗糙的内质网中未观察到齐斯特奈。在成熟阶段的成成木细胞中，金标签位于荷叶边边界区域的木间空间，蔬菜和真空中。这些观察结果表明，在器官培养系统中，牙齿细菌的牙釉质蛋白的定位与体内相似。该器官培养系统可用于研究牙釉质形成机理。（3）我们获得了对小鼠搪瓷蛋白的Ratmouse Chimea单克隆抗体。特异性免疫染色，牙釉质和小鼠成成布的远端部分。较少的