Dynamic Multi-Spectral Tomography for Kinetic Microspecimen

我们利用新的光谱学和时间切片技术开发了显微测量方法，以观察细胞和毛细管中的化学反应和动力学。本项目的具体成果如下：1。四波长激光CT显微镜的发展：为了获得标本的光谱信息和三维图像，在我们研制的光学CT显微镜中引入了四波长激光作为光源。我们验证了可以同时获得光谱信息和三维分布。四眼CT显微镜的研制：研制了一种用于观察运动细胞或活体的四眼CT显微镜。该系统同时获取4个投影，然后通过非负约束重构算法成功获得40 μ m间隔的切片图像。相CT显微镜的发展：通常动物细胞是透明的，染色是一个先决条件。我们开发了一种相CT显微镜来观察没有这种染色过程的活细胞。利用多幅相衬图像重建了三维折射率分布。本显微镜观察烟草细胞培养情况。实验结果表明，该系统能较好地重建切片图像。荧光图谱分析显微镜：为了用显微镜观察标本的动态，我们建立了一种分析显微镜，可以在样品图像上绘制荧光寿命图。我们尝试将该系统应用于测量样品的动态响应，从混合物中识别组分，并使用该测绘显微镜分离每种组分的区域。

We developed microscopic measurement methods utilizing new spectroscopic and time-slicing techniques in order to observe chemical response and dynamics in cell and capillary. The detailed results of this project are as followings :1. Development of Four-Wavelength Laser CT Microscope : In order to obtain spectroscopic information of specimens as well as the 3-D image, a four-wavelength laser was introduced as an optical source into the optical CT microscope we develop. We verified that both spectroscopic information and 3-D distribution could be obtained simultaneously.2. Development of Four-Eye CT Microscope : We developed a four-eye CT microscope for observing moving cells or living organism. In this system four projections were acquired simultaneously, and then 40-mum interval slice images were successfully obtained by a non-negative constrained reconstruction algorithm.3. Development of Phase CT Microscope : Usually animal cells are transparent and staining is required as a prior process. We developed a phase CT microscope for observing alive cells without this staining process. The 3-D refractive-index distribution is reconstructed from several phase contrast images. Cultured tobacco cells were observed with this microscope. It was verified that the slice images were well reconstructed with the system.4. Fluorescence-Mapping Analysis Microscopy : For observing the dynamics of specimens with a microscope, we built an analysis microscope which could map fluorescence lifetime on the sample images. We tried to apply this system to measure dynamic response of specimen, to identify components from a mixture, and to separate area of each components with this mapping microscope.