Dynamic Multi-Spectral Tomography for Kinetic Microspecimen

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We developed microscopic measurement methods utilizing new spectroscopic and time-slicing techniques in order to observe chemical response and dynamics in cell and capillary. The detailed results of this project are as followings :1. Development of Four-Wavelength Laser CT Microscope : In order to obtain spectroscopic information of specimens as well as the 3-D image, a four-wavelength laser was introduced as an optical source into the optical CT microscope we develop. We verified that both spectroscopic information and 3-D distribution could be obtained simultaneously.2. Development of Four-Eye CT Microscope : We developed a four-eye CT microscope for observing moving cells or living organism. In this system four projections were acquired simultaneously, and then 40-mum interval slice images were successfully obtained by a non-negative constrained reconstruction algorithm.3. Development of Phase CT Microscope : Usually animal cells are transparent and staining is required as a prior process. We developed a phase CT microscope for observing alive cells without this staining process. The 3-D refractive-index distribution is reconstructed from several phase contrast images. Cultured tobacco cells were observed with this microscope. It was verified that the slice images were well reconstructed with the system.4. Fluorescence-Mapping Analysis Microscopy : For observing the dynamics of specimens with a microscope, we built an analysis microscope which could map fluorescence lifetime on the sample images. We tried to apply this system to measure dynamic response of specimen, to identify components from a mixture, and to separate area of each components with this mapping microscope.