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In Vitro and in Vivo Nonthorombogenicity of Microdomain Polymers

微域聚合物的体外和体内非血栓形成性

基本信息

批准号：
01490023
负责人：
SAKURAI Yasuhisa
金额：
$ 3.97万
依托单位：
Institute of Biomedical Engineering, Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

We have already reported that block copolymers composed of 2-hydroxyethyl methacylate (HEMA) and styrene (St), having hydrophilic/hydrophobic microdomain structures, demonstrated excellent nonthorombogenicty. In this study, we discuss about relationship between surface structure and activation of platelets by using well-defined HEMA-St block copolymer. The surface structure of block copolymers were estimated by TEM observation, ESCA measurement and contact angle measurement. The lamella structure was clearly observed by TEM and domain spacings were from 100 to 600A. ESCA and contact angle measurement suggested that St segment was concentrated on air surface at dry state. After soaking polymers into water, however, wetability of polymer surface increase and contact angle of polymer surface show almost same value as that of PHEMA. Nonthombogenicity of block copolymers were measured by column method. As the domain spacing was altered, the platelet adhesion exhibited minimum value at 160A of micro domain spacing. Furthermore, to clarify effects of membrane movement on platelets adhesion on polymer surface, platelets were treated with low temperature and colchichine In the block copolymer coated glass bead column, the amount of eluted platelet show no deference between colchichine treated PRP and control PRP. This results indicate that platelets membrane movement was suppressed without drug when platelet contact with the micro domain structure and the active adhesion of platelet is not progressed. Further more, as in vivo application, new skin grafting technique with using artificial blood tubes as saphenous blood tubes was developed.

我们已经报道，由甲基丙烯酸2-羟乙酯（HEMA）和苯乙烯（St）组成的嵌段共聚物具有亲水/疏水微区结构，表现出优异的非血栓形成性。在本研究中，我们通过使用明确的 HEMA-St 嵌段共聚物讨论表面结构与血小板活化之间的关系。通过TEM观察、ESCA测量和接触角测量来估计嵌段共聚物的表面结构。通过 TEM 可以清楚地观察到片层结构，域间距为 100 至 600A。 ESCA和接触角测量表明干燥状态下St段集中在空气表面。然而，将聚合物浸泡在水中后，聚合物表面的润湿性增加，聚合物表面的接触角显示出与PHEMA几乎相同的值。通过柱法测量嵌段共聚物的非凝血性。当微域间距改变时，血小板粘附在微域间距为160A时表现出最小值。此外，为了阐明膜运动对血小板在聚合物表面粘附的影响，用低温和秋水仙碱处理血小板。在嵌段共聚物包被的玻璃珠柱中，洗脱的血小板量在经秋水仙碱处理的PRP和对照PRP之间没有显示差异。该结果表明，当血小板与微域结构接触时，在没有药物的情况下，血小板膜运动受到抑制，并且血小板的主动粘附不进行。此外，作为体内应用，还开发了以人工血管作为隐血管的植皮新技术。