Studies on the Biosynthesis of Blood Group Substances in the Tissues

我们研究了组织中血型物质的生物合成，获得了以下结果：1）用凝集素染色法和免疫染色法测定了福尔马林固定和石蜡包埋的人和动物组织中血型物质糖链的化学结构。用上述方法测定了组织切片中同种抗原物质A、B和H的分布。2)大肠癌组织中血型物质的生物合成非常活跃，而癌上皮细胞和腺泡细胞不产生任何血型物质。这些结果表明，A和B基因在内源和外源因素的作用下失活。3)为了证明血型物质的嵌合现象，我们制作了实验动物模型。利用该模型检测了不同抗原（血型物质）在组织中的存在和分布百分比。我们还可以检查在组织分化模型中异常染色体是在哪个步骤产生的。4)本文的方法对输血事故的诊断有一定的参考价值。

We examined the biosynthesis of blood group substances in tissues and obtained the following results: 1) The chemical structure of carbohydrate chains of blood group substances which exist in formalin-fixed and paraffin-embedded human and animal tissues, was determined by both lectin staining and immunological staining methods using exo- and endo-glycosidase. Distribution of isoantigenic substances, A, B and H in the tissue-sections was also determined by the methods mentioned above. 2) Biosynthesis of blood group substances was very active in cancerrous tissues of large intestine, but cancerous epithelial and acinal cells did not produce any blood group substances. These results indicate that A and B gene are inactivated by endogenous and exogenous factors. 3) We made experimental animal model for proof of mosaic and chimera phenomena of blood group substances. The existance and distribution percentage of different antigens (blood group substances) in tissues were examined by use of the model. We can also examine in what step abnormal chromosomes were produced during tissue differentiation model. 4) Our methods which mentiond in (1) are useful for diagnosis of accidental cases of blood transfusion.