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Genetic analysis of a closed herd of Japanese Black cattle in Tajima district using DNA polymorphisms

利用 DNA 多态性对但马地区封闭群黑毛日本牛进行遗传分析

基本信息

批准号：
02454093
负责人：
GOTO Nobuo
金额：
$ 3.65万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454093/
关键词：
DNA fingerprint Japanese Black cattle parentage identification RFLP bovine lipoprotein lipase cDNA 但馬牛個体識別

项目摘要

To utilize a closed herd of 'Tajima cattle' having high quality beef for genetic improvement of Japanese Black cattle effectively, genetic constitution of the herd was investigated by using DNA polymorphism analysis. The analysis was also applied to parentage identification.1. DNA fingerprinting was examined with a M13 phage DNA probe in the cattle of 42 families extracted from the herd. The parentage of each family was identified by using some restriction endonucleases such as Hae III.2. A linear regression of Genetic Similarity level(X) estimated from DNA fingerprints on relationship coefficient(Y) yielded the equation, Y = 1.149X - 0.026, with highly significant correlation coefficient,indicating that DNA fingerprinting could be applied to estimate the relation coefficient in cattle.3. Cluster analysis based on banding patterns in DNA fingerprints showed that the herd is roughly divided into two groups. This result depended mainly on the frequencies of two bands(minisatellite loci).4. Using bovine cDNA of lipoprotein lypase as a probe, restriction fragment length polymorphisms(RFLP) have been examined in the herd. The 1.3kb probe(5'-half containing open reading frame) gave distinctive RFLPs with 5 restriction enzymes such as Pst I. Types of RFLP being given with each restriction enzyme were closely related to each other and expressed as haplotypes A and B, which suggests all RFLP types are linking. It was shown that Aand B RFLP types are codominantly inherited. Heifers of 116 Japanese Black of the herd were classified into 3 groups, AA(15 heads), AB(65) and BB(35), with the gene frequencies of 0.418 (haplotype A) and 0.582 (B).

为了有效利用具有优质牛肉的田岛牛封闭群进行日本黑牛的遗传改良，采用DNA多态性分析对该群体的遗传构成进行了研究。该分析也被应用于亲子鉴定。用M13噬菌体DNA探针对从该牛群中提取的42个家系的牛进行了DNA指纹分析。用Hae Ⅲ等限制性内切酶鉴定各家系的亲缘关系。用DNA指纹图谱估算的遗传相似度（X）与相关系数（Y）进行线性回归，得到方程Y = 1.149X - 0.026，相关系数达到极显著水平，表明DNA指纹图谱技术可以用于估算牛的相关系数.基于DNA指纹带型的聚类分析表明，牛群大致分为两组。这一结果主要取决于两条带（小卫星位点）的频率.以牛脂蛋白酶cDNA为探针，研究了限制性片段长度多态性（RFLP）。1.3kb的探针（5 '-半含开放阅读框）在Pst Ⅰ等5种限制性内切酶的作用下产生了特异性的RFLP。每种限制性内切酶的RFLP类型彼此密切相关，并表示为单倍型A和B，这表明所有RFLP类型都是连锁的。结果表明，A和B RFLP类型是共显性遗传。将116头日本黑母牛分为AA型（15头）、AB型（65头）和BB型（35头）3组，基因频率分别为0.418（单倍型A）和0.582（B）。