Genetic Relationships Between the Colonies of Japanese White Rabbits and Establishment of the Their Standard Strain

日本白兔群体亲缘关系及其标准品系的建立

• 批准号: 63880013
• 负责人: GOTO Nobuo
• 金额: $ 5.63万
• 依托单位: Faculty of Agriculture, Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63880013/
• 关键词: Japanese White Rabbit; Mandible analysis; UTLIEF; DNA fingerprint; M13ファ-ジ; DNAフィンガ-プリント; 系統の同定; 類縁関係; 主成分分析

Japanese White Rabbits have been widely used for various tests of conditions such as pyrogen, dermal and eye irritation and teratogenesis. Tests using these rabbits, however, are not so accurate, because they are not controlled genetically like laboratory mice and rats. It is, therefore, necessary to develop genetically controlled standard strain so as to be representative of Japanese White Rabbit. The present project was carried out to clarify the relationships between the colonies of Japanese White Rabbits by applying mandible analysis and ultrathin layer isoelectric focusing for blood and to establish the 'standard strain' from the results of the analyses.The analyses revealed that Japanese White Rabbits in produced Japan were genetically divided into six groups. From this result, establishment of 'standard strain' of Japanese White Rabbit was seemed to be best taking the following two methods.1. To select six colonies from the respective groups and produce the six-way hybrid. And to maintain the hybrid as a closed colony from generation to generation.2. To select the most genetically controlled colony from a group consisting of the most colonies examined, and designate the colony as 'standard strain'.

日本白色家兔已广泛用于各种条件试验，如热原、皮肤和眼睛刺激以及致畸作用。然而，使用这些兔子进行的测试并不那么准确，因为它们不像实验室小鼠和大鼠那样受到遗传控制。因此，有必要建立具有代表性的日本白色兔遗传控制标准品系。本研究通过对日本白色兔的下颌骨进行分析，并对血液进行等电聚焦，以确定日本白色兔群体之间的关系，并根据分析结果建立“标准品系”。从这一结果来看，日本白色家兔“标准品系”的建立似乎最好采取以下两种方法。从各组中选择六个菌落并产生六向杂交种。并使杂种一代一代地保持封闭的群体状态.从检查的最多菌落组成的组中选择遗传控制最好的菌落，并将该殖民地指定为“标准菌株”。

项目成果

福沢崇行: "超薄層ポリアクリルアミドゲル等電点電気泳動による日本白色種ウサギ血清蛋白質多型の分析ー主としてコロニ-間の比較ー" ABRI(Animal Blood Group Research Information). 18. 31-36 (1990)

Takayuki Fukuzawa：“通过超薄聚丙烯酰胺凝胶等电点分析日本白兔血清蛋白多态性 - 主要在菌落之间进行比较”ABRI（动物血型研究信息）18. 31-36（1990）。

Takayuki, Fukuzawa, Nobuo Goto and Soichi Tsuji: "Plasma protein polymorphism of Japanese White Rabbit analyzed by ultrathin layer polyacrylamide gel electrophoresis Comparison between several breeding colonies-" Anim. Blood Res. Inform.18. 31-36 (1990)

Takayuki，Fukuzawa，Nobuo Goto和Soichi Tsuji：“通过超薄层聚丙烯酰胺凝胶电泳分析日本白兔血浆蛋白多态性，几个繁殖群体之间的比较-”Anim。

福沢 崇行: "超薄層ポリアクリルアミドゲル等電点電気泳動による日本白色種ウサギ血清蛋白質多型の分析ー主としてコロニ-間の比較ー" Anim.Blood Grp.Res.Inform.18. 31-36 (1990)

Takayuki Fukuzawa：“通过超薄聚丙烯酰胺凝胶等电点分析日本白兔血清蛋白多态性 - 菌落之间的比较”Anim.Blood Grp.Res.Inform.18（1990）。