Molecular epidemiology of type C influenza

丙型流感的分子流行病学

• 批准号: 02454180
• 负责人: NAKAMURA Kiyoto
• 金额: $ 4.1万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454180/
• 关键词: Influenza C virus; HE protein; antigenic structure; antigenic variation; monoclonal antibody; ヘムアグルチニン・エステラ-ゼ; 抗原地図; 中和エピト-プ; 抗原変異株; 糖蛋白質

1. We produced a total of 37 monoclonal antibodies (MAbs) against the hemagglutinin-esterase (HE) protein of influenza C virus, ten with hemagglutination-inhibition, hemolysis inhibition, and neutralization activities (group A) and 27 without the activities (group B). Operational and topological analyses with these antibodies revealed that at least nine non-over-lapping or partially overlapping antigenic sites were present on the HE protein, four recognized by group A MAbs (A-1-A-4) and five by group B (B-1 - B-5).2. Neutralization-resistant variants of influenza C virus were selected with group A MAbs against four different antigenic sites (A-1-A-4), and their HE genes were sequenced to identify amino acid residues important for the integrity of each site. Although variants for antigenic site A-2 had a change at position 367, all substitutions in the variants for sites A-1, A-3, and A-4 occurred in the central region of the HE1 subunit spanning amino acid position 178 to 283. It was a … More lso found that many of the variants selected with antibodies to sites A-1 and A-3 were clustered within or near one of the three variable regions revealed previously by comparing amino acid sequences of the HEs among various influenza C isolates, which supports the notion that influenza C virus, like human influenza A viruses, may be exposed to a significant amount of immunological pressure.3. Sixteen influenza C strains isolated in Yamagata City between April 1988 and May 1990 were compared by antigenic analysis with anti-HE HAbs and oligonucleotide mapping of total vRNAs. The results showed that the isolates could be classified into two groups : seven isolates were closely related to the influenza C strain isolated in 1981 in Aichi prefecture, (Aichi/1/81), and the remaining nine were very similar to the strain isolated in the same year in Yamagata prefecture (Yamagata/1/81). Thus it appears that two evolutionary linages of influenza C virus, different markedly from each other in the antigenicity of HE, were selected presumably by immunological pressure and became predominant at least in Yamagata city. Less

1. 我们共制备了37种针对C型流感病毒血凝素酯酶（HE）蛋白的单克隆抗体（mab），其中10种具有血凝抑制、溶血抑制和中和活性（a组），27种无活性（B组）。这些抗体的操作和拓扑分析显示，HE蛋白上至少存在9个不重叠或部分重叠的抗原位点，其中4个被A组单克隆抗体识别（A-1-A-4）， 5个被B组单克隆抗体识别（B-1 - B-5）。用A组单克隆抗体（mab）对4个不同的抗原位点（A-1-A-4）选择具有中和抗性的C型流感病毒变体，并对其HE基因进行测序，以确定对每个位点的完整性至关重要的氨基酸残基。虽然抗原位点a -2的变异体在367位发生了变化，但位点a -1、a -3和a -4的变异体的所有替换都发生在HE1亚基的中心区域，横跨氨基酸位置178至283。More还发现，许多选择了a -1和a -3位点抗体的变异都聚集在之前通过比较不同C型流感分离株的HEs氨基酸序列所揭示的三个可变区域之一内或附近，这支持了C型流感病毒与人类a型流感病毒一样可能暴露于大量免疫压力下的观点。对1988年4月至1990年5月在山形市分离的16株C型流感病毒进行了抗he HAbs抗原分析和总vrna的寡核苷酸定位。结果表明，分离株可分为两类，其中7株与1981年爱知县（爱知/1/81）分离株密切相关，其余9株与同年在山形县（山形/1/81）分离株非常相似。因此，两种在HE抗原性上明显不同的C型流感病毒进化谱系可能是由免疫压力选择的，至少在山形市占主导地位。少

项目成果

西村 秀一,原 正啓,菅原 勘悦,北目 文郎,滝口 和彦,梅津 由子,外崎 昭,中村 喜代人: "Characterization of the cordーlike strustures emerging from the surgace of influenza C virusーinfected cells" Virology. 179. 179-188 (1990)

Shuichi Nishimura、Masahiro Hara、Kanetsu Sukawara、Fumio Kitame、Kazuhiko Takiguchi、Yuko Umezu、Akira Tonosaki、Kiyoto Nakamura：“丙型流感病毒感染细胞表面出现的索状结构的特征”病毒学 179。 179-188 (1990)

M. Matsuzaki, K. Sugawara, K. Adachi, S. Hongo, H. Nishimura, F. Kitame, K. Nakamura.: "Location of neutralizing epitopes on the hemagglutinin-esterase protein of influenza C virus." Virology.

M. Matsuzaki、K. Sukawara、K. Adachi、S. Hongo、H. Nishimura、F. Kitame、K. Nakamura.：“丙型流感病毒血凝素酯酶蛋白上中和表位的位置。”

菅原 勘悦,西村 秀一,本郷 誠治,北目 文郎,中村 喜代人: "Antigenic characterization of the nucleoprotein and matrix protein of influenza C virus with monoclonal antibodies" Journal of General Virology. 72. 103-109 (1991)

Kanetsu Sugarara、Shuichi Nishimura、Seiji Hongo、Fumio Kitame、Kiyoto Nakamura：“用单克隆抗体对丙型流感病毒核蛋白和基质蛋白进行抗原表征”普通病毒学杂志 72. 103-109 (1991)。

H. Nishimura, M. Hara, K. Sugawara, F. Kitame, K. Takiguchi, Y. Umetsu, A. Tonosaki, K. Nakamura.: "Characterization of the cord-like structures emerging from the surface of influenza C virus-infected cells." Virology. 179. 179-188 (1990)

H. Nishimura、M. Hara、K. Sukawara、F. Kitame、K. Takiguchi、Y. Umetsu、A. Tonosaki、K. Nakamura.：“丙型流感病毒表面出现的索状结构的特征-

松嵜 正實: "C型インフルエンザウイルスによる実験室内感染" 山形医学. 8. 41-51 (1990)

Masami Matsuzaki：“丙型流感病毒的实验室感染”Yamagata Medical。 8. 41-51 (1990)