权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the significance of antigenic variation in influenza C epidemiology

丙型流感流行病学中抗原变异意义的研究

基本信息

批准号：
63480156
负责人：
NAKAMURA Kiyoto
金额：
$ 4.22万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1. Antigenic and genetic analyses of three influenza C strains, isolated in the Kinki district in 1982-1983, showed that they were very similar to one another but dissimilar to any of the strains isolated in Japan before 1982. A comparison of the HE gene-sequences of these strains with those of the previously published ones revealed that the Kinki isolates had a high degree of homology with the virus isolated in 1980 in the United States (Mississippi/1/80). The isolates from Kinki and Mississippi could be distinguished from the previous isolates in serological tests with polyclonal sera. These observations suggest that an influenza C variant closely related to Mississippi/1/80 has spread rapidly in Kinki in 1982-1983 presumably because this imported virus was largely different in antigenicity from the previously prevalent ones.2. Antigenic and genetic analyses of eight influenia C strains isolated in Japan in 1985-1989 were also performed. One of the two 1985 isolates was similar to Mississippi/1/80 whereas the other was dissimilar to any of the previously isolated strains. Six strains isolated after 1986, however, all resembled the virus isolated in 1981 in the Aichi prefecture (Aichi/1/81), raising the possibility that in 1986-1989 the Aichi/l/81-like variant may have possessed an epidemiological advantage over any of the other coexisting strains.3. The technique for isolation of influenza C virus has been confined to the amniotic inoculation of embryonated hen's eggs that is too laborious for routine use. Therefore we attempted to develop a tissue culture method for isolation of the virus and found that a human melanoma cell line (HMV- II) is useful for this purpose.

1. 对1982-1983年在近畿地区分离出的三种丙型流感病毒株的抗原和遗传分析表明，它们彼此非常相似，但与1982年之前在日本分离出的任何一种病毒株不同。将这些菌株的HE基因序列与先前发表的病毒序列进行比较，发现Kinki分离株与1980年在美国（Mississippi/1/80）分离的病毒具有高度的同源性。经多克隆血清血清学检测，Kinki和Mississippi分离株可与以往分离株区分。这些观察结果表明，一种与Mississippi/1/80密切相关的C型流感变体在1982-1983年在Kinki迅速传播，可能是因为这种输入病毒在抗原性上与以前流行的病毒有很大不同。还对1985-1989年在日本分离的8株丙型流感病毒株进行了抗原和遗传分析。两个1985年分离株中有一个与密西西比/1/80相似，而另一个与以前分离的菌株不同。然而，1986年以后分离出的6株毒株都与1981年在爱知县分离出的病毒（爱知/1/81）相似，这提高了1986-1989年爱知/1/81样变种可能比任何其他共存毒株具有流行病学优势的可能性。分离丙型流感病毒的技术一直局限于对胚胎鸡蛋进行羊膜接种，这对于常规使用来说过于费力。因此，我们尝试开发一种组织培养方法来分离病毒，并发现人类黑色素瘤细胞系（HMV- II）可用于此目的。