Intercelluar Ca level measured by IP4BP and PI response

通过 IP4BP 和 PI 响应测量细胞间 Ca 水平

• 批准号: 02454250
• 负责人: KAWAGUCHI Hideaki
• 金额: $ 4.35万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454250/
• 关键词: Inositol-4phosphate; Intracellular Ca; IP4 binding protein; signal transduction; Phospholipse C; イノシト-ル4リン酸; 細胞内カリシウム; イノシト-ル4リン酸結合蛋白質; ホスホリパ-ゼC; イノシト-ル4リン酸、

It is known that inositol (1,4,5)-trisphosphate (IP_3) stimulates Ca^<2+> release from sarcoplasmic reticulum (SR) in several tissues, but in cardiac myocytes the effect of IP_3 on Ca^<2+> release from sarcoplasmic reticulum is controversial. In this experitment, we have proved that (1,4,5)-IP_3 stimulates Ca^<2+> release from cardiac myocyte sarcoplasmic reticulum by digital-image method in signle cell. In cardiac myocyte treated with saponin, Ca^<2+> release stimulated by 10 muM (1,4,5)-IP_3 was detected by fura-2. In ^<45>Ca^<2+> prelabeled sarcoplasmic reticulum, the maximal Ca^<2+> release was achieved at 10 muM IP_3 for 1 min incubation. The release of Ca^<2+> was higher is SHR sarcoplasmic reticulum than in that of WKY.But it is not clear whether this phenomenon has a significant role in cardiac hypertrophy.

三磷酸肌醇（inositol（1，4，5）-trisphosphate，IP_3）可刺激多种组织的肌浆网（sarcoplasma reticulum，SR）释放Ca ^2+，但对心肌细胞的影响尚存在争议。本实验用数字图象法证实了（1，4，5）-IP_3刺激心肌细胞肌浆网Ca^<2+>释放的作用。在用皂甙处理的心肌细胞中，用fura-2检测到10 μ M（1，4，5）-IP_3刺激的Ca^<2+>释放。在<45>预先标记的肌浆网中，10 μ M IP_3孵育1分钟可使Ca^&lt;2+&gt;释放达到最大。SHR心肌肌浆网Ca^&lt;2+&gt;释放量高于WKY，但这一现象是否在心肌肥厚中起重要作用尚不清楚。

项目成果

Makiguchi M, Kawaguchi H, Tamura M, Yasuda H: "Effect of fatty acid and fatty acid binding protein on ventricular arrythmia" Cardiovascular drugs and therapy. 5. 753-762 (1991)

Makiguchi M、Kawaguchi H、Tamura M、Yasuda H：“脂肪酸和脂肪酸结合蛋白对室性心律失常的影响”心血管药物和治疗。

H Kawaguchi: "Enclothelin stimulates aigiotensin I to angiotensin II conversion in cultured pulmonary artery endothelial cells" Journal Moll Cell Caudiol. 22. 839-842 (1990)

H Kawaguchi：“内皮素刺激培养的肺动脉内皮细胞中血管紧张素 I 向血管紧张素 II 的转化”Journal Moll Cell Caudiol。

Okamato H, Kawaguchi H, Togashi H, Minami M, Saito H, and Yasuda H: "Effect of Co enzyme Q on structual alteration in the renal membrane of stroke prone spontaneously hypertensive rats (SHRSP)" Biochem Med Metabol Biol. 45. 216-226 (1991)

Okamato H、Kawaguchi H、Togashi H、Minami M、Saito H 和 Yasuda H：“辅酶 Q 对中风易发性自发性高血压大鼠 (SHRSP) 肾膜结构改变的影响”Biochem Med Metabol Biol。

Makiguchi M: "Effect of fatty acid and fatty acid binding protein on rentricular arrythmia" Candiovascular drugs and therapy. 5. 753-762 (1991)

Makiguchi M：“脂肪酸和脂肪酸结合蛋白对房室心律失常的影响”血管血管药物和治疗。

H Kawaguchi: "Effect of endothelin on angiotensin converting enzyme activity in caltured pulmonary artery endothelial cells" Journal of Hypertension. 9. 171-174 (1991)

H Kawaguchi：“内皮素对培养的肺动脉内皮细胞中血管紧张素转换酶活性的影响”高血压杂志。