Long time preservation of whole & split liver with intermittent perfusion

整体长时间保存

• 批准号: 02454295
• 负责人: KOYAMA Kenji
• 金额: $ 4.29万
• 依托单位: Akita University, Akita, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454295/
• 关键词: Liver Preservation; DNA Damage & Repair; Parenchimal & Nonparenchimal Cell of the Liver; Liver Mitochondria; Intermittent Liver Perfusion; DNA損傷・修復

The liver for transplantation is preserved in cold solution, however, the viability or transplantability decreases with the lapse of time. Prolongation of the time limit for preservation is strongly requested for the convenience of the transplantation.First, the viability tests of the liver preserved were studied using rats, and DNA damage and mitochondrial function, especially, the preservation of ATP synthesis after heat shock loading were strongly recommended.Next, for prolongation of time limit, intermission perfusion for 1 hr with normothermic buffer solution was adopted to the rat liver during preservation in cold UW solution. Damage of cell membrane, protein synthesis and mitochondrial function were investigated, and following, results were obtained. Mitochondrial function was significantly improved by the perfusion. However, membrane damage of both parechymal and nonparenchymal cells measured by in situ trypanblue uptake was not improved by the perfusion. Furthermore, protein synthesis was not increased by perfusion.These results indicate that the mitochondrial improvement was not enough to increase the protein synthesis of hepatocytes. However, when oxygen carrier of artificial blood was used as perfusate instead of UW solution mitochondrial function was highly reserved after presevation. for longer time. Therefore, some devices in both perfusate and perfusion conditions will provide the prolongation of the time limit of preservation.

用于移植的肝脏保存在冷溶液中，但随着时间的推移，肝脏的活力或可移植性会降低。为方便移植，强烈要求延长保存期限。首先，采用大鼠研究保存肝脏的活力测试，强烈推荐热休克负荷后的DNA损伤和线粒体功能，特别是ATP合成的保存。其次，为了延长时限，在冷UW溶液中保存的大鼠肝脏采用常温缓冲液间歇灌注1hr。对细胞膜损伤、蛋白质合成和线粒体功能进行了研究，得到了以下结果。灌注后线粒体功能明显改善。然而，原位锥虫蓝摄取测定的实质细胞和非实质细胞的膜损伤均未因灌注而改善。此外，灌注不增加蛋白质合成。这些结果表明，线粒体的改善并不足以增加肝细胞的蛋白质合成。然而，当以含氧载体人工血液代替UW溶液作为灌注液时，保存后线粒体功能高度保留。更长的时间。因此，有些装置在灌注和灌注两种情况下都会延长保存时限。

项目成果

Shugo Kashima: "Establishment of isolated hypothermic perfusion of the liver. (Japanese with English summary)" Akita J. Med. 17. 729-736 (1990)

Shugo Kashima：“肝脏隔离低温灌注的建立。（日语与英语摘要）”Akita J. Med。

佐藤 勤: "肝実質細胞・非実質細胞の核.DNA損傷修復からみた肝保存法の評価." 移植. 26. 349-357 (1991)

Tsutomu Sato：“肝实质细胞和非实质细胞的细胞核。从 DNA 损伤修复的角度评估肝脏保存方法。” 26. 349-357 (1991)。

Kenji Koyama: "Ratioal procedure of hepatic vascular exclusion with hypothemic liver perfusion for complicated hepatic surgery" Hepato-Gastroenterology. 39. (1992)

Kenji Koyama：“用于复杂肝脏手术的肝血管排除和低温肝脏灌注的合理程序”肝胃肠病学。

Tsutom Sato: "Effect of normothermic intermission perfusion on the liver during preservation in cold UW solution" Transplantation.

Tsutom Sato：“常温间歇灌注对冷 UW 溶液保存期间肝脏的影响”移植。

Kenji Koyama: "Rational procedure of hepatic vascular exclusion with hypothermic liver perfusion for complicated hepatic surgery." Hepato-Gastroenterology. 39. (1992)

Kenji Koyama：“复杂肝脏手术中肝血管阻断和低温肝脏灌注的合理程序。”