Long time preservation of whole & split liver with intermittent perfusion

用于移植的肝脏保存在冷溶液中，然而，随着时间的推移，存活力或可移植性降低。为了便于移植，延长保存时限是迫切需要的。首先，用大鼠研究了保存肝脏的活力试验，并强烈建议在热休克负荷后保存DNA损伤和线粒体功能，特别是ATP合成。其次，为了延长时限，采用常温缓冲液间断灌注1小时的方法，对大鼠肝脏进行UW冷液保存。通过对细胞膜损伤、蛋白质合成和线粒体功能的研究，得到以下结果。线粒体功能通过灌注显著改善。然而，通过原位台盼蓝摄取测量的实质和非实质细胞的膜损伤并没有通过灌注得到改善。此外，蛋白质合成没有增加灌注。这些结果表明，线粒体的改善不足以增加肝细胞的蛋白质合成。而用人工血的氧载体代替UW液作为灌流液，保存后线粒体功能得到高度保留。更长的时间因此，在灌注液和灌注条件下的一些装置将提供保存时限的延长。

The liver for transplantation is preserved in cold solution, however, the viability or transplantability decreases with the lapse of time. Prolongation of the time limit for preservation is strongly requested for the convenience of the transplantation.First, the viability tests of the liver preserved were studied using rats, and DNA damage and mitochondrial function, especially, the preservation of ATP synthesis after heat shock loading were strongly recommended.Next, for prolongation of time limit, intermission perfusion for 1 hr with normothermic buffer solution was adopted to the rat liver during preservation in cold UW solution. Damage of cell membrane, protein synthesis and mitochondrial function were investigated, and following, results were obtained. Mitochondrial function was significantly improved by the perfusion. However, membrane damage of both parechymal and nonparenchymal cells measured by in situ trypanblue uptake was not improved by the perfusion. Furthermore, protein synthesis was not increased by perfusion.These results indicate that the mitochondrial improvement was not enough to increase the protein synthesis of hepatocytes. However, when oxygen carrier of artificial blood was used as perfusate instead of UW solution mitochondrial function was highly reserved after presevation. for longer time. Therefore, some devices in both perfusate and perfusion conditions will provide the prolongation of the time limit of preservation.