Investigation on development of cell differentiation therapy for gastroenterological carcinomas.

观察了环磷酸二丁酯(db-cAMP)和8-溴环腺苷(8-br-cAMP)对人胰腺癌和胆道癌裸鼠连续移植瘤生长和增殖活性的影响。胰腺癌经cAMP类似物处理后，细胞周期显示S期增加，G1期减少，但对组织分化、肿瘤生长抑制、核分裂指数和DNA指数无明显影响。在胆道癌和胆囊癌中，胆囊癌的肿瘤生长在给药14天时受到抑制，但在28天的研究中没有受到抑制。细胞周期分析显示胆管癌细胞和胆囊癌细胞周期中G1期减少，S期增多。结论：cAMP类似物可通过延缓细胞周期进程而抑制细胞生长，cAMP类似物对癌细胞增殖的调节作用可能与其他抗癌药物联用有助于肿瘤的治疗。

The effect of dibutyryl cyclic adenosine monophosphate(db-cAMP) and 8-bromo cyclic AMP(8-br-cAMP) on tumor growth and proliferating activity of human pancreas and biliary tract cancers serially transplanted in nude mice was evaluated. Of pancreas cancer, cell cycle analysis by flow cytometry showed increase of S-phase and decrease of G1-phase, while histological differentiation, tumor growth inhibition, mitotic index and DNA index were not significantly affected after cAMP analogue administration. Of bile duct cancer and gallbladder cancer, tumor growth of gallbladder cancer was suppressed by 14 days administration of 8-br-cAMO, but not for the 28 days study. Analysis of cell cycle showed decrease of G1-phase and increase of S-phase in bile duct cancer and gallbladder cancers.In conclusion, the cAMP analogues can produce growth inhibition by slowing down cell cycle progression, and regulatory effect of cAMP analogues on proliferation of cancer cells may contribute to the cancer management in combination with other anticancer treatments.