Investigation on development of cell differentiation therapy for gastroenterological carcinomas.

胃肠道肿瘤细胞分化治疗的进展研究。

• 批准号: 04454325
• 负责人: KOYAMA Kenji
• 金额: $ 4.35万
• 依托单位: Akita University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454325/
• 关键词: cell differentiation; dB-cAMP; 8-Br-cAMP; pancreatic cancer; biliary tract cancer; cell cycle; anticancer effect; nude mice; dB-cAMP; 8-Br-cAMP; 胆道癌; 悪性度; DNA

The effect of dibutyryl cyclic adenosine monophosphate(db-cAMP) and 8-bromo cyclic AMP(8-br-cAMP) on tumor growth and proliferating activity of human pancreas and biliary tract cancers serially transplanted in nude mice was evaluated. Of pancreas cancer, cell cycle analysis by flow cytometry showed increase of S-phase and decrease of G1-phase, while histological differentiation, tumor growth inhibition, mitotic index and DNA index were not significantly affected after cAMP analogue administration. Of bile duct cancer and gallbladder cancer, tumor growth of gallbladder cancer was suppressed by 14 days administration of 8-br-cAMO, but not for the 28 days study. Analysis of cell cycle showed decrease of G1-phase and increase of S-phase in bile duct cancer and gallbladder cancers.In conclusion, the cAMP analogues can produce growth inhibition by slowing down cell cycle progression, and regulatory effect of cAMP analogues on proliferation of cancer cells may contribute to the cancer management in combination with other anticancer treatments.

本文观察了二丁酰环磷酸腺苷（db-cAMP）和8-溴环磷酸腺苷（8-br-cAMP）对人胰胆管癌裸鼠移植瘤生长和增殖活性的影响。流式细胞仪分析胰腺癌细胞周期显示S期细胞增多，G1期细胞减少，而cAMP类似物对胰腺癌的组织分化、肿瘤生长抑制、有丝分裂指数和DNA指数无明显影响。在胆管癌和胆囊癌中，8-br-cAMO给药14天可抑制胆囊癌的肿瘤生长，但在28天研究中则不然。细胞周期分析显示，胆管癌和胆囊癌细胞G1期减少，S期增加，提示cAMP类似物可通过减慢细胞周期进程而产生生长抑制作用，其对肿瘤细胞增殖的调节作用可能有助于与其他抗癌药物联合治疗。

项目成果

Akiko Umezawa,et al: "Anticancer effect of dibutyryl cyclic AMP(dB-cAMP) on human pancreatic cancer xenografted into mude mice" Gastroenterologia Japonica. 28. 135 (1993)

Akiko Umezawa等人：“二丁酰环AMP(dB-cAMP)对人胰腺癌异种移植到mude小鼠体内的抗癌作用”Gastroenterologia Japonica。

Jun-ichi Tanaka, Akiko Umezawa, Yujiro Kato, Ken-ichi Miyasaki, Yasuhiko Sato, Kenji Koyama: Differenticaiton therapy for human bile duct cancer, Recent Advances in Management of Digestive Cancers. Springer-Verlag, Tokyo, 810-812 (1993)

Jun-ichi Tanaka、Akiko Umezawa、Yujiro Kato、Ken-ichi Miyasaki、Yasuhiko Sato、Kenji Koyama：人类胆管癌的鉴别治疗，消化道癌症治疗的最新进展。

Jun-ichi Tanaka,et al: "Differentiation therapy for human bile duct cancer" Recent Advances in Management of Digestive Cancers. 810-812 (1993)

Jun-ichi Tanaka 等人：“人类胆管癌的分化治疗”消化道癌症治疗的最新进展。

Akiko Umezawa, Jun-ichi Tanaka, Yasuhiko Sato, Hitoshi Seki, Yujiro Kato, Ken-ichi Miyasaki, Kenji Koyama: "Anticancer effect of dibutyryl cyclic AMP(dB-cAMP) on human pancreatic cancer xenografted into nude mice" Gastroenterologia Japonica. 28. 135 (1993

Akiko Umezawa、Jun-ichi Tanaka、Yasuhiko Sato、Hitoshi Seki、Yujiro Kato、Ken-ichi Miyasaki、Kenji Koyama：“二丁酰环 AMP（dB-cAMP）对人胰腺癌异种移植到裸鼠体内的抗癌作用”Gastroenterologia Japonica。

Akiko Umezawa,et al: "Anticancer effect of dibutyryl cyclic AMP(dB-cAMP)on human pancreatic cancer xenografted into nude mice." Gastroenterologia Japonica. 28. 135 (1993)

Akiko Umezawa等人：“二丁酰环AMP(dB-cAMP)对人胰腺癌裸鼠异种移植的抗癌作用”。