A study on chemosensitivity and enhanced efficacy with hyperthermia of head and neck cancer

1.头颈部肿瘤的化疗敏感性研究采用快速胸腺嘧啶核苷掺入法(RTIA)，对头颈部实体瘤的化疗敏感性进行了研究。结果显示：RTIA法对167例患者322例标本的可评价率为51%(85/167)，对51例患者288例标本的可评价率为94%(45/51)。MMC、CBDCA、CDDP、5-FU、PEP、ADM的药敏符合率分别为83%、75%、70%、70%、60%、29%。ADM中的不一致是一个问题。在临床相关性方面，RTIA法10例总的预测准确率为70%，而ATP法6例为83%。总体而言，耐药的预测准确率高于敏感性，与其他部门的报道一样，ATP法具有可评价性高、检测周期短、可预测性高等优点，优于RTIA。温热联合化疗对头颈部肿瘤的增强疗效(1)用V-79细胞研究温热联合化疗对顺铂和卡铂的增效作用。两种处理均使耐热细胞的热敏性略有提高。(2)采用C3H小鼠移植鳞癌模型，观察不同时间热疗联合不同能量光动力疗法(PDT)的治疗效果。因此，无论治疗顺序如何，当将高剂量治疗作为首选时，联合作用最强。

1. Chemosensitivity of head and neck cancer Using the rapid thymidine incorporation assay (RTIA), chemosensitivity of head and neck solid cancer was studied. The results showed that the evaluable rate of RTIA for 167 patients, 322 specimens, was 51% (85/167), while that of ATP assay for 51 patients, 288 specimens, was 94% (45/51). Co- incident rates of drug sensitivity from the both assay were 83% in MMC, 75% in CBDCA, 70% in CDDP, 70% in5-FU, 60% in PEP, 29% in ADM, respectively. The inconsistency in ADM was a problem. As for the clinical correlation, the overall predictive accuracy of RTIA was 70% in 10 cases, while that of ATP assay was 83% in 6 cases. In general, the predictive accuracy for resistance was higher than for sensitivity like previous reports from the other department ATP assay is better than RTIA for its high evaluability, short assay period and high predictability.2. Enhanced efficacy combined with hyperthermia for head and neck cancer(1) Combined efficacy of hyperthermia with chemotherapy Whether CDDP and CBDCA can show enhanced efficacy with hyperthermia was studied using V-79 cells. Both treatment induced a slight enhancement in thermosensitivity of thermotolerant cells. Simultaneous heat treatment at 42^0 C with either drug inhibited the thermotolerance developed during 42^0 C heating.(2) Combined efficacy of hyperthermia with the photodynamic therapy (PDT) Using C3H mice transplanted squamous cell carcinoma, the therapeutic effect of different periods of hyperthermia in combination with different energies of PDT was investigated. As a result, the combination effect was strongest when high dose therapy was used as the first choice, irrespective of the order of the treatment