A study on chemosensitivity and enhanced efficacy with hyperthermia of head and neck cancer

1. 采用快速胸腺嘧啶掺入试验（RTIA）研究了头颈部实体癌的化学敏感性。结果167例322例标本RTIA可评估率为51%(85/167)，51例288例标本ATP可评估率为94%（45/51）。两种方法的药敏Co-发生率分别为：MMC 83%、CBDCA 75%、CDDP 70%、5- fu 70%、PEP 60%、ADM 29%。ADM的不一致性是一个问题。在临床相关性方面，10例RTIA的总体预测准确率为70%，6例ATP检测的总体预测准确率为83%。总体而言，ATP法对耐药的预测准确度高于对敏感性的预测，其可评价性高、测定周期短、可预测性高，优于RTIA法。(1)热疗联合化疗的疗效采用V-79细胞研究CDDP和CBDCA是否能在热疗的作用下增强疗效。两种处理均能使耐热细胞的热敏性略有增强。用任意一种药物在42^0℃同时热处理，抑制了42^0℃加热过程中产生的耐热性。(2)热疗联合光动力治疗（PDT）以C3H小鼠移植的鳞状细胞癌为研究对象，观察不同时期热疗联合不同能量光动力治疗的治疗效果。结果表明，无论治疗顺序如何，以高剂量治疗为第一选择时，联合效应最强

1. Chemosensitivity of head and neck cancer Using the rapid thymidine incorporation assay (RTIA), chemosensitivity of head and neck solid cancer was studied. The results showed that the evaluable rate of RTIA for 167 patients, 322 specimens, was 51% (85/167), while that of ATP assay for 51 patients, 288 specimens, was 94% (45/51). Co- incident rates of drug sensitivity from the both assay were 83% in MMC, 75% in CBDCA, 70% in CDDP, 70% in5-FU, 60% in PEP, 29% in ADM, respectively. The inconsistency in ADM was a problem. As for the clinical correlation, the overall predictive accuracy of RTIA was 70% in 10 cases, while that of ATP assay was 83% in 6 cases. In general, the predictive accuracy for resistance was higher than for sensitivity like previous reports from the other department ATP assay is better than RTIA for its high evaluability, short assay period and high predictability.2. Enhanced efficacy combined with hyperthermia for head and neck cancer(1) Combined efficacy of hyperthermia with chemotherapy Whether CDDP and CBDCA can show enhanced efficacy with hyperthermia was studied using V-79 cells. Both treatment induced a slight enhancement in thermosensitivity of thermotolerant cells. Simultaneous heat treatment at 42^0 C with either drug inhibited the thermotolerance developed during 42^0 C heating.(2) Combined efficacy of hyperthermia with the photodynamic therapy (PDT) Using C3H mice transplanted squamous cell carcinoma, the therapeutic effect of different periods of hyperthermia in combination with different energies of PDT was investigated. As a result, the combination effect was strongest when high dose therapy was used as the first choice, irrespective of the order of the treatment