A study on chemosensitivity and enhanced efficacy with hyperthermia of head and neck cancer

头颈癌化疗敏感性及热疗增效研究

• 批准号: 02454396
• 负责人: SAITO Hitoshi
• 金额: $ 2.24万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454396/
• 关键词: Head and neck cancer; Chemosensitivity; Rapid thymidine incorporation assay; ATP assay; Clinical correlation; Hyperthermia; Thermotolerance; Photodynamic therapy; 頭頸部悪性腫瘍; 温熱化学療法

1. Chemosensitivity of head and neck cancer Using the rapid thymidine incorporation assay (RTIA), chemosensitivity of head and neck solid cancer was studied. The results showed that the evaluable rate of RTIA for 167 patients, 322 specimens, was 51% (85/167), while that of ATP assay for 51 patients, 288 specimens, was 94% (45/51). Co- incident rates of drug sensitivity from the both assay were 83% in MMC, 75% in CBDCA, 70% in CDDP, 70% in5-FU, 60% in PEP, 29% in ADM, respectively. The inconsistency in ADM was a problem. As for the clinical correlation, the overall predictive accuracy of RTIA was 70% in 10 cases, while that of ATP assay was 83% in 6 cases. In general, the predictive accuracy for resistance was higher than for sensitivity like previous reports from the other department ATP assay is better than RTIA for its high evaluability, short assay period and high predictability.2. Enhanced efficacy combined with hyperthermia for head and neck cancer(1) Combined efficacy of hyperthermia with chemotherapy Whether CDDP and CBDCA can show enhanced efficacy with hyperthermia was studied using V-79 cells. Both treatment induced a slight enhancement in thermosensitivity of thermotolerant cells. Simultaneous heat treatment at 42^0 C with either drug inhibited the thermotolerance developed during 42^0 C heating.(2) Combined efficacy of hyperthermia with the photodynamic therapy (PDT) Using C3H mice transplanted squamous cell carcinoma, the therapeutic effect of different periods of hyperthermia in combination with different energies of PDT was investigated. As a result, the combination effect was strongest when high dose therapy was used as the first choice, irrespective of the order of the treatment

1.应用快速胸苷掺入法（RTIA）研究头颈部实体癌的化疗敏感性。结果显示：RTIA法对167例患者322份标本的可评价率为51%（85/167），ATP法对51例患者288份标本的可评价率为94%（45/51）。两种方法的药敏符合率分别为MMC 83%、CBDCA 75%、CDDP 70%、5-FU 70%、PEP 60%、ADM 29%。ADM中的不一致性是一个问题。在临床相关性方面，RTIA的总预测准确率为70%（10例），ATP测定的总预测准确率为83%（6例）。总的来说，耐药预测准确率高于敏感性，与其他部门的报道相似，ATP法可评价性高，检测周期短，预测性强，优于RTIA法.（1）热疗与化疗的联合效应使用V-79细胞研究CDDP和CBDCA是否可以显示热疗的增强效应。两种处理均诱导耐热细胞的热敏性略有增强。在42^0 ℃下同时用任一种药物进行热处理都能抑制在42^0 ℃加热过程中产生的耐热性。(2)热疗联合光动力疗法（PDT）的疗效观察以C3 H小鼠移植性鳞癌为实验对象，观察不同时间热疗联合不同能量PDT的治疗效果。因此，无论治疗顺序如何，当高剂量治疗作为首选时，联合效应最强

项目成果

斎藤 等: "化学療法ー方法と適応、限界、感受性試験からみた薬剤の選択." 医学のあゆみ. 152. 363-366 (1990)

Saito 等人：“化疗 - 从方法、适应症、局限性和药敏试验的角度进行药物选择。”152. 363-366 (1990)。

斎藤 等: "頭頚部悪性腫瘍の化学療法(一週一話)." 日本医事新報. 3488. 129 (1991)

Saito 等：“头颈部恶性肿瘤的化疗（每周一次）。Nihon Iji Shinpo。” 3488. 129 (1991)

Saito H: "Chemosensitivity of head and neck cancer with the rapid thymidine incorporation assay and its clinical application." Eur Arch Otorhinolaryngol. 249. 400-403 (1992)

Saito H：“头颈癌的化学敏感性快速胸苷掺入测定及其临床应用。”

Saito H.: "Chemosensitivity of larngeal cancer." Recent advances in bronchoesphagology, Elsevier Science Publishers. (1990)

Saito H.：“喉癌的化学敏感性。”

斎藤 等: "頭頚部進行癌に対する化学療法の役割" 耳鼻. 38. 733-741 (1992)

Saito 等人：“化疗对晚期头颈癌的作用”Otorhinose 38. 733-741 (1992)