Effects on Retinal Pigment Epithelium in Vitro of Dopa and Oxygen.

多巴和氧气对体外视网膜色素上皮的影响。

• 批准号: 02454409
• 负责人: TANAKA Yasuhiko
• 金额: $ 4.35万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454409/
• 关键词: Retinal Pigment Epithelium; Melanin; in vitro; Free radicals; Cell growth; Superoxide dismutase; Cell cycle; Dopa; Superoxide dismutase; ド-パ; ス-パ-オキサイドディスミュタ-ゼ; 吸光度; カタラ-ゼ

Purpose. It is possible that the effects of L-dopa and oxygen on retinal pigment epithelial (RPE) cells are dependent on tyrosinase activities because melanin precursors and free radicals are produced during melanin synthesis by tyrosinase. We compared these cytotoxic effects of L-dopa and oxygen on cell growth, superoxide dismutase (SOD) activity, and cell cycle of melanotic RPE cells with amelanotic RPE cells.Methods. We conducted cell count, SOD activity, and flow cytometric DNA analysis of RPE cells exposed to L-dopa (100 muM and 250 muM) changing oxygen concentrations (20%, 10%, and 5%).Results. Melanotic RPE cells were more tolerant of toxicity of dopa-oxidation than amelanotic RPE cells. SOD activity of RPE cells was increased by exposure to dopa regardless of melanin granules. A dose-dependent effect of L-dopa to arrest the cell cycle (the S phase) was more pronounced in melanotic RPE cells than in amelanotic RPE cells, and oxygen caused arrest in the G_1 phase.

目的.左旋多巴和氧对视网膜色素上皮细胞的作用可能依赖于酪氨酸酶的活性，因为酪氨酸酶在黑色素合成过程中产生黑色素前体和自由基。我们比较了L-多巴和氧对色素沉着和无色素沉着RPE细胞生长、超氧化物歧化酶（SOD）活性和细胞周期的影响。通过改变氧浓度（20%、10%和5%），对暴露于L-dopa（100 μ M和250 μ M）的RPE细胞进行细胞计数、SOD活性和流式细胞术DNA分析。黑色素性RPE细胞比无色素性RPE细胞更耐受多巴氧化的毒性。无论黑色素颗粒如何，多巴暴露均可增加RPE细胞的SOD活性。L-dopa对色素性RPE细胞的S期阻滞作用比无色素性RPE细胞更为明显，且呈剂量依赖性，氧可使细胞阻滞于G_1期。

项目成果

Akeo,K.,Nagasaki,K.,Tanaka,Y.,Curran, S.A.Dorey,C.K.: "Comparison of Effects of Oxygen and Antioxidative Enzymes on Cell Growth bertween Retinal Pigment Epithelial Cells and Vascular Endothelial Cell in vitro" Ophthalmic Research. 24. 357-364 (1992)

Akeo,K.、Nagasaki,K.、Tanaka,Y.、Curran, S.A.Dorey,C.K.：“体外视网膜色素上皮细胞和血管内皮细胞之间氧和抗氧化酶对细胞生长的影响比较”眼科研究。

明尾 潔: "網膜色素上皮細胞の増殖と活性酸素" 活性酸素・フリーラジカル. 3. 402-410 (1992)

Kiyoshi Akio：“视网膜色素上皮细胞的增殖和活性氧”活性氧和自由基3. 402-410 (1992)。

明尾 潔: "網膜色素上皮細胞の増殖と活性酸素" 活性酸素,フリーラジカル. 3. 402-410 (1992)

Kiyoshi Akio：“视网膜色素上皮细胞的增殖和活性氧”活性氧，自由基3. 402-410 (1992)。

Akeo,K., Nagasaki,K., Tanaka,Y., Curran,S.A., Dorey,C.K.: "Comparison of Effects of Oxygen and Antioxidative Enzymes on Cell Growth between Retinal Pigment Epithelial Cells and Vascular Endothelial Cells in vitro" Ophthalmic Research. 24. 357-364 (1992)

Akeo,K.、Nagasaki,K.、Tanaka,Y.、Curran,S.A.、Dorey,C.K.：“氧和抗氧化酶对体外视网膜色素上皮细胞和血管内皮细胞生长的影响的比较”眼科研究。

Akeo,K.;Nagasaki,K.;Tanaka,Y.;Curran,S.A.;Dorey,C.K.: "Comparison of Effects of Oxygen and Antioxidative Enzymes on Cell Growth between Retinal Pigment Epithelial Cells and Vascular Endothelial Cells in vitro" Ophthalmic Research. 24. 357-364 (1992)

Akeo,K.；Nagasaki,K.；Tanaka,Y.；Curran,S.A.；Dorey,C.K.：“氧和抗氧化酶对视网膜色素上皮细胞和血管内皮细胞体外细胞生长的影响比较”眼科研究。