Molecular Characterization of host-specific and –unspecific properties of IdeC, the IgG-specific protease of Streptococcus canis

IdeC（犬链球菌 IgG 特异性蛋白酶）宿主特异性和非特异性特性的分子表征

• 批准号: 430573905
• 负责人: Professor Dr. Marcus Fulde
• 金额: --
• 依托单位: Institut für Mikrobiologie und Tierseuchen (WE07)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/430573905?language=en
• 关键词: Molecular; Characterization; host; specific; unspecific

Similar to Streptococcus (S.) canis, a facultative pathogen generally found associated with dogs and cats, many of the more than 50 different Streptococcus species show remarkable host specificities. To date, little is known about the molecular bases behind the host-specificities, although immunoglobulin (Ig)-specific proteases of the IdeS/Mac family appear to play an important role. This project will concentrate on IdeC, the IgG-specific protease of S. canis. Using molecular biological and protein biochemistry methods, we will determine the mechanism of the host-specificity of IdeC for canine and feline IgG, and using rational protein design, determine how the host-specificity can be altered and/or extended. In addition, many of the IdeS/Mac family proteases harbor an RGD motif in the protein sequence. As RGD motifs are implicated in general, species-independent recognition of host cell integrins, we will also determine the role for IdeC as an adhesin or invasin of host cells using molecular and cell biology methods.

与链球菌（S.）犬链球菌是一种兼性病原体，通常发现与狗和猫相关，50多种不同的链球菌物种中的许多显示出显著的宿主特异性。迄今为止，很少有人知道背后的宿主特异性的分子基础，虽然免疫球蛋白（IG）特异性蛋白酶的IdeS/Mac家庭似乎发挥了重要作用。本项目将重点研究链球菌IgG特异性蛋白酶IdeC。罐。使用分子生物学和蛋白质生物化学方法，我们将确定犬和猫IgG的IdeC的宿主特异性的机制，并使用合理的蛋白质设计，确定如何改变和/或延长宿主特异性。此外，许多IdeS/Mac家族蛋白酶在蛋白质序列中具有RGD基序。由于RGD基序通常涉及宿主细胞整合素的物种独立识别，我们还将使用分子和细胞生物学方法确定IdeC作为宿主细胞粘附素或侵袭素的作用。