Synthesis of Polysaccharides by Using Enzyme Catalyst

酶催化合成多糖

• 批准号: 03453114
• 负责人: KOBAYASHI Shiro
• 金额: $ 3.97万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453114/
• 关键词: Enzymatic Polymerization; Polysaccharide; Oligosaccharide; Cellulase; Cellooligosaccharide; alpha-D-Maltosyl Fluoride; Pullulanase; プルラナーゼ; セルロ-ス; セルラ-ゼ; フッ化βーDーセロビオシル; マルトオリゴ糖; アミラ-ゼ; フッ化αーDーマルトシル

The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of beta-D-cellobiosyl fluoride as substrate for cellulase in a mixed solvent of acetonitrile/acetate buffer (pH 5,5:1)(enzymatic polymerization). Under reaction condition of a higher substrate concentration or higher acetonitrile concentration, cellooligosaccharides were produced predominantly. The present method of using a glycosyl fluoride as substrate for a hydrolytic enzyme in an organic/water mixed solvent has successfully been applied to synthesis of maltooligosaccharides and unnatural oligosaccharides.A new methodology for regio and stereoselective synthesis of cellooligosaccharide derivatives has been developed by utilizing the following two enzymatic reactions : A cellulase-catalyzed stereoselective lactosylation of an alkyl cellobioside giving rise to a new oligosaccharide having a D-galactose unit at the nonreducing end and a beta-galactosidase-catalyzed regioselective cleavage of the terminal D-galactose unit of the product.

以纤维素酶的底物β-D-纤维二糖氟为底物，在乙腈/醋酸酯缓冲液(pH 5，5：1)混合溶剂(酶促聚合)中进行缩合，首次实现了纤维素酶的非生物合成途径。在较高底物浓度或较高乙腈浓度的反应条件下，主要生成纤维低聚糖。在有机/水混合溶剂中，利用糖基氟化物作为水解酶的底物，成功地合成了麦芽寡糖和非天然低聚糖。通过纤维素酶催化的烷基纤维二糖糖苷的立体选择性乳糖化反应和β-半乳糖苷酶催化的末端D-半乳糖单元的区域选择性裂解，发展了一种区域和立体选择性合成纤维低聚糖衍生物的新方法。

项目成果

S.Kovayashi: "Enzymatic Polymerization:The First in Vitro Synthesis of Cellulose via Nonbiosynthetic Path Catalyzed by Cellulase" Makromol.Chem.Macromol.Symp.54-55. 509-518 (1992)

S.Kovayashi：“酶聚合：通过纤维素酶催化的非生物合成途径首次体外合成纤维素”Makromol.Chem.Macromol.Symp.54-55。

S.Kobayashi: "Enzymatic Polymerization and Oligomerization" Adv.Polym.Sci.

S.Kobayashi：“酶促聚合和寡聚”Adv.Polym.Sci。

S.Kobayashi: "Novel Method for Polysaccharide Synthesis Using an Enzyme:The First in Vitro Synthesis of Cellulose via a Nonbiosynthetic Path Utilizing Cellulase as Catalyst" J.Am.Chem.Soc.113. 3079-3084 (1991)

S.Kobayashi：“使用酶合成多糖的新方法：利用纤维素酶作为催化剂通过非生物合成途径首次体外合成纤维素”J.Am.Chem.Soc.113。

S.Shoda: "A Facile Enzymatic Synthesis of Cellooligosaccha-ride Bdrivatives Using β-D-Lactosyl Fluoride" Carbohydr.Res.

S.Shoda：“使用 β-D-乳糖酰氟轻松酶法合成纤维寡糖 B 衍生物”CarboHydr.Res。

S.Kobayashi: "Enzymatic Polymerization : The First in Vitro Synthesis of Cellulose via Nonbiosynthetic Path Catalyzed by Cellulase" Makromol. Chem. Macromol. Symp.54/55. 509-518 (1992)

S.Kobayashi：“酶聚合：通过纤维素酶催化的非生物合成路径首次体外合成纤维素”Makromol。