Search for xylosylglucose-type sugar chains by pyridylamination

通过吡啶胺化搜索木糖基葡萄糖型糖链

• 批准号: 03453164
• 负责人: HASE Sumihiro
• 金额: $ 4.42万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453164/
• 关键词: Xylosylglucose; Glycoprotein; HPLC; Pyridylamination; Fluorescence labeling; Sugar chain; キシロ-ス; 尿; 肝臓; 少糖

We have showed the existence of xylosylglucosyl-0-serine type sugar chain in glycoproteins of animals, and its complete structure was determined (Hase et al. 1990). The biological significance of the new type sugar chains is not known so far, but the sugar chain has unique chemical structure together with the xylose residue in the linkage position of proteoglycans. The sugar chains are found only on the serine residue linked by O-glycosidically in blood coagulation factors, where the sugar chains are linked to the EGF-like domain of the glycoproteins. To know the distribution of the sugar chains in animals, detection of the sugar chains was carried out. For this purpose pyridylamination was used to detect very small amounts of sugar chains. Xylosylglucose type sugar chains were found mainly in brain, blood, and kidney of mouse and ox. And the sugar chains were also found in cell line HepG2, especially in the soluble fraction of the cells. In human urine the trisaccharide was also found. The procedure was developed by which sugar chains of the glycoproteins are detected after purification of the glycoproteins by gel-electro-phoresis, which is a base procedure to search glycoproteins with the sugar chains.

我们已经证明在动物的糖蛋白中存在木糖葡萄糖基-O-丝氨酸型糖链，并确定了其完整结构（Hase et al. 1990）。这种新型糖链的生物学意义目前尚不清楚，但其具有独特的化学结构，在蛋白聚糖的连接位置上还有木糖残基。在凝血因子中，糖链仅存在于通过O-糖苷连接的丝氨酸残基上，其中糖链与糖蛋白的EGF样结构域连接。为了了解糖链在动物体内的分布，对糖链进行了检测。为此，使用吡啶胺化来检测非常少量的糖链。木糖葡萄糖型糖链主要存在于小鼠和牛的脑、血液和肾脏中。在细胞株HepG 2中也发现了糖链的存在，尤其是在细胞的可溶性部分。在人尿中也发现了三糖，并建立了用凝胶电泳纯化糖蛋白后检测糖蛋白糖链的方法，这是寻找含糖蛋白的基本方法。

项目成果

長束 俊治: "糖-その多様性を探る(化学増刊122)ピリジルアミノ化法による糖鎖の高感度分析" 化学同人, 8 (1992)

永冢俊二：“糖 - 探索其多样性（化学特别版 122）通过吡啶胺化方法对糖链进行高灵敏度分析” 化学同人，8（1992）

長谷 純宏: "複合糖質の糖鎖研究における新しい方法論とその展望ーピリジルアミノ化の役割ー" 生化学63, 11 (1991)

Yoshihiro Hase：“复杂碳水化合物碳水化合物链研究的新方法和前景——吡啶胺化的作用” 生物化学 63, 11 (1991)

長束 俊治: "糖ーその多様性を探る(化学増刊122)ピリジルアミノ化法による糖鎖の高感度分析" 化学同人, 8 (1992)

永冢俊二：“探索糖的多样性（化学特别版122）通过吡啶胺化方法对糖链进行高灵敏度分析”化学同人，8（1992）

K.Fukase: "Synthesis of New Serine-Linked Oligosaccharides in Blood-Clotting Factors VII and IX and Protein Z. The Syntheses of O- alpha-D-Xylopyranosyl-(1 *3)-D-glycopyranose, O-alpha-D-Xylopyranosyl-(1 *3)- O-alpha-D-xylopyranosyl-(1 *3)-D-glucopyranose

K.Fukase：“凝血因子 VII 和 IX 以及蛋白 Z 中新型丝氨酸连接寡糖的合成。O-α-D-吡喃木糖基-(1 *3)-D-吡喃葡萄糖、O-α-D 的合成

K.Omichi: "Studies on the Active Site of Human αーAmylase:Examination of the Third Subsite S3' of the AglyconeーBinding Site by Control of Substrate Binding Mode" J.Biochem.111. 4-7 (1992)

K.Omichi：“人 α-淀粉酶活性位点的研究：通过控制底物结合模式检查糖苷配基结合位点的第三个亚位点 S3”J.Biochem.111（1992）。