GLYCOprotein N-glycosylation from non-life to eukaryotes: a Doctoral Network to expand the knowledge on a ubiquitous posttranslational modification

从非生命到真核生物的糖蛋白 N-糖基化：扩展普遍存在的翻译后修饰知识的博士网络

• 批准号: EP/Y032527/1
• 负责人: Benjamin Schumann
• 金额: $ 33.22万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY032527%2F1
• 关键词: GLYCOprotein; glycosylation; non; life; eukaryotes

The GLYCO-N training network aims at training Doctoral Candidates (DCs) to acquire the skills to develop different innovative strategies to 1) understand the diversity and structural complexity of archaeal, microalgal and viral N-glycosylation and 2) harness this knowledge for new solutions in biomedicine and biotechnology. Protein N-glycosylation, or the attachment of oligo- and polysaccharides at specific asparagine residues, is conserved throughout life, and is now observed even in the viral world. In contrast to eukaryotes, whose well-studied N-glycosylation machineries are relatively simple, archaea, microalgae and bacteria utilize a wide variety of monosaccharides to create a wealth of structurally diverse N-glycans, and the same holds true for some recently discovered viruses. Because protein glycosylation occurs far downstream of protein synthesis the complexity and diversity in N-glycan structures are poorly understood in detail. This holds true specifically for Nglycosylation events that are the subject of the GLYCO-N program: those in archaea, microalgae and viruses. Understanding of the how and why of N-glycosylation in archaea, microalgae and viruses will open up many possibilities ranging from drug discovery (antivirals) to biotechnology (glycoprotein and glycoprocessing enzyme engineering for materials and life sciences). The GLYCO-N network brings together a diverse group of glycobiology researchers with world-leading expertise in microbiology, (bio)organic chemistry, computational and structural biology, bioinformatics and chemical biology. The GLYCO-N DCs will have their own individual project with one GLYCO-N expert and will, through research internships, be exposed to complementary Glycoscience. All individual PhD projects, while rooted in fundamental science, have a practical application, either in biotechnology or in biomedicine, as will be explored through secondments with our associated partners

GLYCO-N培训网络旨在培训博士生（DCs）获得开发不同创新策略的技能，以1）了解古细菌，微藻和病毒N-糖基化的多样性和结构复杂性，2）利用这些知识为生物医学和生物技术提供新的解决方案。蛋白质N-糖基化，或在特定的天冬酰胺残基上连接寡糖和多糖，在整个生命过程中是保守的，现在甚至在病毒世界中也可以观察到。与真核生物相比，其充分研究的N-糖基化机制相对简单，古细菌，微藻和细菌利用各种单糖来产生丰富的结构多样的N-聚糖，这同样适用于一些最近发现的病毒。由于蛋白质糖基化发生在蛋白质合成的下游，因此对N-聚糖结构的复杂性和多样性的详细了解很少。这特别适用于GLYCO-N计划的主题N糖基化事件：古细菌，微藻和病毒中的那些。了解古细菌、微藻和病毒中N-糖基化的方式和原因将开辟许多可能性，从药物发现（抗病毒药）到生物技术（材料和生命科学的糖蛋白和糖加工酶工程）。GLYCO-N网络汇集了在微生物学，（生物）有机化学，计算和结构生物学，生物信息学和化学生物学方面具有世界领先专业知识的糖生物学研究人员。GLYCO-N DCs将与一名GLYCO-N专家开展自己的个人项目，并将通过研究实习接触补充糖科学。所有个人博士项目，而植根于基础科学，有一个实际应用，无论是在生物技术或生物医学，将通过借调与我们的相关合作伙伴探索