Function of lipid transfer protein and its homologus proteins in plant cells
植物细胞中脂质转运蛋白及其同源蛋白的功能
基本信息
- 批准号:03454007
- 负责人:
- 金额:$ 4.16万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Function of lipid transfer protein (LTP) and its homologus proteinsLPT was isolated as a protein which transfers polar lipids between biomembranes in vitro, but it is unlikely that LTP functions in vivo for intracellular lipid trasfer. LTPs did not occur in cytosol but in density vesicles in cells and cell walls. The expression of LTP genes and the accumulation of LTPs were observed in the surface area of developing organs such as embryos, endosperms and primordia of leaves and floweres. In addition, LTPs have the capacities of binding fatty acids and acylCoAs. From these results. LTP seems to functions in vivo as a carrier of wax or cutin monomers. This assumption was supported by evidence that wax synthesis induced by UV irradiation in cucmber leaves was followed by the accumulation of LTPs in the cuticles. On the other hand , the localization of LTPs in density vesicles in castor bean cotyledon cells and assembly of these vesicles into a vacuoles remains unsolved.2. Cloning of LTP genes and its homologus protein genesThere are organ-specific isoforms of LTP, A - D, in castor bean seedlings. We cloned the isoform C gene by PCR method using the isoform C _cDNA.Intron sturucture of the isoform C gene has been determined, but its promoter structure has not, because of unsuccessfulness in isolating the upstream region of LTP gene. Several clones have been isolated from genomic library prepared from castor bean cotyledons and the analysis of nucleotides is in progress.Eight cysteines in LTP sequence were conservative. To examine the role of these cysteines for binding of LTP to fatty acid, the isoform C _cDNA was modified to yield the isoform C with serines instead the cysteines and the modified LTP is now to be expressed.
1. 脂质转移蛋白(LTP)及其同源蛋白slpt在体外被分离为在生物膜之间转移极性脂质的蛋白,但LTP在体内不太可能在细胞内转移脂质。ltp不发生在细胞质中,而是发生在细胞和细胞壁的密度囊泡中。在胚胎、胚乳和叶、花的原基等发育器官的表面区域观察到LTP基因的表达和积累。此外,LTPs还具有结合脂肪酸和酰基辅酶a的能力。从这些结果中。LTP似乎在体内作为蜡或角质单体的载体起作用。这一假设得到了紫外线照射诱导的黄瓜叶片蜡合成和角质层LTPs积累的支持。另一方面,LTPs在蓖麻子叶细胞密度囊泡中的定位以及这些囊泡组装成液泡的机制仍未得到解决。LTP基因及其同源蛋白基因的克隆蓖麻幼苗LTP存在器官特异性亚型A - D。利用C _cDNA,用PCR方法克隆了C基因。C基因的内含子结构已经确定,但其启动子结构尚未确定,原因是未能成功分离LTP基因的上游区域。从蓖麻子叶基因组文库中分离到多个克隆,核苷酸分析正在进行中。LTP序列中有8个半胱氨酸是保守的。为了研究这些半胱氨酸在LTP与脂肪酸结合中的作用,对C _cDNA异构体进行了修饰,得到了带丝氨酸的C异构体而不是半胱氨酸,现在修饰后的LTP将被表达。
项目成果
期刊论文数量(42)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Arao, T.and Yamada, M.: "Biosynthesis of fatty acids in Pheodactylm tricornutum" Phytochemistry. (in press).
Arao, T. 和 Yamada, M.:“三角褐指藻中脂肪酸的生物合成”植物化学。
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- 影响因子:0
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- 通讯作者:
Tsuboi, S., Osafune, T., Tsuneki, R., Nishimura, M.and Yamada, M.: "Nonspecific lipid transfer protein in castor bean cells -- Subcellular localization and a possible role in lipid metabolism" J.Biochemistry. 111. 500-508 (1992)
Tsuboi, S.、Osafune, T.、Tsuneki, R.、Nishimura, M. 和 Yamada, M.:“蓖麻子细胞中的非特异性脂质转移蛋白 - 亚细胞定位和脂质代谢中的可能作用”J.Biochemistry。
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Sakaki,T.,Tanaka,K.,Yamada,M.: "General metabolic changes in leaf lipids in response to ozone" Plant and Cell Physiology. 35. 53-62 (1994)
Sakaki,T.、Tanaka,K.、Yamada,M.:“叶脂响应臭氧的一般代谢变化”植物和细胞生理学。
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- 影响因子:0
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Arao,T.and Yamada,M.: "Biosynthesis of fatty acids in Phaedactylm tricornutum" Phytochemistry. (in press).
Arao,T. 和 Yamada,M.:“三角褐藻中脂肪酸的生物合成”植物化学。
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- 影响因子:0
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Tsuboi,S.,Osafune,T.,Tsugeki,R.,Nishimura,M.and Yamada,M.: "Nonspecific lipid transfer protein in castor bean cotyledon cells Subcellular localization and a possible role in lipid metabolism" Journal of Biochemistry. 110. 823-831 (1991)
Tsuboi,S.,Osafune,T.,Tsugeki,R.,Nishimura,M.和 Yamada,M.:“蓖麻子子叶细胞中的非特异性脂质转移蛋白亚细胞定位及其在脂质代谢中的可能作用”生物化学杂志。
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YAMADA Mitsuhiro其他文献
YAMADA Mitsuhiro的其他文献
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25540119 - 财政年份:2013
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$ 4.16万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Identification of somatic stem cells in an alveolar epithelial cell population using a label-retaining strategy
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24591148 - 财政年份:2012
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The role of lysophosphatidic acid in acute lung injury
溶血磷脂酸在急性肺损伤中的作用
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22790745 - 财政年份:2010
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$ 4.16万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
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