Studies on Enzyme Inhibitors of Microalgae

微藻酶抑制剂的研究

• 批准号: 03454087
• 负责人: YAMAGUCHI Kataumi
• 金额: $ 4.16万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454087/
• 关键词: Microalgae; Microcystis aeruginosa; Enzyme inhibitor; Protease inhibitor; Trypsin inhibitor; Peptide; Erastase inhibitor; Micropeptin; 藍藻; 酵素阻害; トリプシン; エラスタ-ゼ; パパイン; キモトリプシン

In the screening of serine protease inhibitors in microalgae, the blue green alga Microcystis aeruginosa (NIES-100), which produced the angiotensin converting enzyme inhibitive peptide, micropeptin showed potent inhibition against trypsin. Micropeptins A(1) and B(2) isolated from the cultured alga. The gross structures of micropeptins A and B were elucidated on the basis of the chemical degradation and extensive NMR analyses including ^1H-^1H COSY, HMQC, HOHAHA, HMQC-HOHAHA and ROESY. Micropeptins contained Ahp like related cyclic depsipeptide dolastatin 13 isolated from Dolabella auricularia. They inhibited trypsin and plasmin at very low concentration.

在微藻丝氨酸蛋白酶抑制剂的筛选中，产生血管紧张素转换酶抑制肽的蓝藻铜绿微囊藻(NIES-100)对胰酶有较强的抑制作用。从培养的藻类中分离得到微肽A(1)和B(2)。通过化学降解和1H-1HCOSY、HMQC、HOHAHA、HMQC-HOHAHA和ROESY等核磁共振分析，确定了微肽A和B的大体结构。微肽含有AHP相关的环状去脂肽Dolastatin 13，它是从耳壳中分离得到的。它们在很低的浓度下抑制胰酶和纤溶酶。

项目成果

Katsumi Yamaguchi: "Screening of protease inhibitory activities in microalgae" Journal of Applied Phycology.

Katsumi Yamaguchi：“微藻中蛋白酶抑制活性的筛选”应用藻类学杂志。

Katsumi Yamaguchi: "New trypsin inhibitors from the blueーgreen alga Microcystis aeruginosa" Tetrahedron Letters.

Katsumi Yamaguchi：“来自蓝绿藻铜绿微囊藻的新型胰蛋白酶抑制剂”四面体快报。