Structure, Localization and Function of Proteoglycan Expressed Specifically in the Developing Rat Brain

(1)我们提出了一种单克隆抗体，命名为MAb-1G 2，它识别神经蛋白聚糖，脑特异性硫酸软骨素蛋白聚糖与220 kDa的核心糖蛋白，其C-末端的一半在大鼠大脑。(2)在兔中产生抗体（PAb-291），其合成肽的氨基酸序列与neurocan的N-末端一半的一部分（氨基酸编号483至498）相同。(3)使用这些抗体，nuerocan显示主要存在于未成熟的脑中，但在成熟的脑中作为两个蛋白聚糖片段存在。(4)免疫细胞化学和原位杂交实验表明，在体感区的桶形成过程中，在转录水平上减少了neurocan的合成。(5)使用纯化的神经肽聚糖和这些抗体的细胞生物学研究表明，神经肽聚糖参与调节神经突的分支和延伸。

(1) We raised a monoclonal antibody, named MAb-1G2, which recognized neurocan, a brain-specific chondroitin sulfate proteoglycan with a 220 kDa core glycoprotein, and its C-terminal half in the rat brain.(2) Antibodies (PAb-291) were raised in the rabbit to a synthetic peptide whose amino acid sequence is identical to a portion (amino acid No. 483 to 498) of N-terminal half of neurocan.(3) Using these antibodies, nuerocan was shown to exist predominantly in inmature brain, but to exist as two proteoglycan fragments in mature brain.(4) Both immunocytochemical and in situ hybridization experiments revealed that the synthesis of neurocan was reduced at transcriptional level during barrel formation in the somatosensory area.(5) Cell biological studies using purified nerocan and these antibodies suggested that neurocan was involved in regulation of branching and elongation of neurites.