Structure, Localization and Function of Proteoglycan Expressed Specifically in the Developing Rat Brain

发育中的大鼠大脑中特异表达的蛋白多糖的结构、定位和功能

• 批准号: 03454121
• 负责人: OOHIRA Atsuhiko
• 金额: $ 2.88万
• 依托单位: Institute for Developmental Research, Aichi Prefectural Colony
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454121/
• 关键词: proteoglycan; brain; development; neurocan; monoclonal antibody; barrel; somatosensory area; in situ hybridization; ガイド分子; コンドロイチン硫酸; 脳の発達; モノクロ-ナル抗体; ラット

(1) We raised a monoclonal antibody, named MAb-1G2, which recognized neurocan, a brain-specific chondroitin sulfate proteoglycan with a 220 kDa core glycoprotein, and its C-terminal half in the rat brain.(2) Antibodies (PAb-291) were raised in the rabbit to a synthetic peptide whose amino acid sequence is identical to a portion (amino acid No. 483 to 498) of N-terminal half of neurocan.(3) Using these antibodies, nuerocan was shown to exist predominantly in inmature brain, but to exist as two proteoglycan fragments in mature brain.(4) Both immunocytochemical and in situ hybridization experiments revealed that the synthesis of neurocan was reduced at transcriptional level during barrel formation in the somatosensory area.(5) Cell biological studies using purified nerocan and these antibodies suggested that neurocan was involved in regulation of branching and elongation of neurites.

(1)我们提出了一种单克隆抗体，命名为MAb-1G 2，它识别神经蛋白聚糖，脑特异性硫酸软骨素蛋白聚糖与220 kDa的核心糖蛋白，其C-末端的一半在大鼠大脑。(2)在兔中产生抗体（PAb-291），其合成肽的氨基酸序列与neurocan的N-末端一半的一部分（氨基酸编号483至498）相同。(3)使用这些抗体，nuerocan显示主要存在于未成熟的脑中，但在成熟的脑中作为两个蛋白聚糖片段存在。(4)免疫细胞化学和原位杂交实验表明，在体感区的桶形成过程中，在转录水平上减少了neurocan的合成。(5)使用纯化的神经肽聚糖和这些抗体的细胞生物学研究表明，神经肽聚糖参与调节神经突的分支和延伸。

项目成果

Katoh-Semba,Ritsuko: "Core proteins of soluble chondroitin sulfate proteoglycans purified from rat brain block the cell cycle of PC12D cells" Journal of Cellular Physiology. 156. 17-23 (1993)

Katoh-Semba，Ritsuko：“从大鼠脑中纯化的可溶性硫酸软骨素蛋白聚糖的核心蛋白阻断 PC12D 细胞的细胞周期”《细胞生理学杂志》。

大平,敦彦(分担執筆): "脳の形態形成とプロテオグリカン。ブレインサイエンス最前線'94" 講談社サイエンティフィク(ブレインサイエンス振興財団編), 197 (1993)

大平敦彦（共同作者）：“脑形态发生和蛋白多糖。脑科学的最前沿94”讲谈社科学（脑科学振兴财团编辑），197（1993）

Maeda, N., Matsui, F., and Oohira, A.: "A chondroitin sulfate proteoglycan that is developmentally regulated in the cerebellar mossy fiber system" Developmental Biology. 151. 564-574 (1992)

Maeda, N.、Matsui, F. 和 Oohira, A.：“一种在小脑苔藓纤维系统中受发育调节的硫酸软骨素蛋白聚糖”发育生物学。

Maeda, N., and Oohira, A.: "Proteoglycan and development of the brain : Functions of brain chondroitin sulfate proteoglycan" Trends in Glycoscience and Glycotechnology. 3. 28-34 (1991)

Maeda, N. 和 Oohira, A.：“蛋白多糖与大脑发育：脑硫酸软骨素蛋白多糖的功能”糖科学和糖技术趋势。

Matsui, F., Oohira, A., Shoji, R., Kariya, Y., and Yoshida, K.: "Biochemical comparison of brain glycosaminoglycans between normal and reeler mutant mice" Nueroscience Research. 16. 287-292 (1993)

Matsui, F.、Oohira, A.、Shoji, R.、Kariya, Y. 和 Yoshida, K.：“正常小鼠和 reeler 突变小鼠脑糖胺聚糖的生化比较”Nueroscience Research。