A study of the alpha-subunits of brain GTP-binding proteins from patients suffering schizophrenic, or Alzheimer's disease

对精神分裂症或阿尔茨海默病患者大脑 GTP 结合蛋白 α 亚基的研究

• 批准号: 03454286
• 负责人: OKADA Fumihiko
• 金额: $ 4.16万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454286/
• 关键词: schizophrenia; Hippocampus; GTP-binding protein, Goalpha, Gi2alpha; conformation; asymmetry; diagnosis x side interaction; SAS analysis; pertussis toxin; GTP結合蛋白質αサブユニット; ウエスターン・ブロット法; G蛋白質遺伝子; アルツハイマ-病; GTP結合蛋白貭αサブユニット; エンザイム イムノ アッセイ; ウェスタ

Concentrations of the alpha subunits of GTP-binding protein, Go (Goalpha) and of Gi2 (Gi2alpha) in 6 areas of control and schizophrenic post-mortem brains were investigated using the highly sensitive enzyme immunoassay method. There was a significant decrease in Goalpha in the hippocampus of the right hemisphere in schizophrenic patients compared to controls; SAS analysis demonstrated a significant diagnosis x side interaction. In other areas of the brain, analysis by grouping under diagnosis, side, age, gender and postmortem delay showed no significant deviations in Goalpha between controls and schizophrenics. The concentrations of Gi2alpha did not differ significantly in any area. These findings contrasted with the results yielded by ADP-ribosylation, which showed decreased pertussis toxin ADP-ribosylated amounts in the hippocampus and putamen of the contralateral (left) hemisphere. The discrepancy might be explained by the existence of differing conformations of Go or Gi1 in the hippocampi of the right and left sides in schizophrenics.

使用高灵敏度酶免疫测定方法研究了对照和精神分裂症死后大脑的 6 个区域中 GTP 结合蛋白 Go (Goalpha) 和 Gi2 (Gi2alpha) 的 α 亚基浓度。与对照组相比，精神分裂症患者右半球海马的 Goalpha 显着下降； SAS 分析证明了显着的诊断 x 侧面相互作用。在大脑的其他区域，根据诊断、侧面、年龄、性别和死后延迟进行分组分析显示，对照组和精神分裂症患者的 Goalpha 没有显着差异。 Gi2alpha 的浓度在任何区域都没有显着差异。这些发现与 ADP-核糖基化产生的结果形成对比，后者显示对侧（左）半球的海马和壳核中百日咳毒素 ADP-核糖基化量减少。这种差异可能是由于精神分裂症患者左右侧海马中存在不同的 Go 或 Gi1 构象。

项目成果

Fumihiko Okada: "Is the tendency to weep one of the most useful indicators for depressed mood?" J.Clin.Psychiatry. 52. 351-352 (1991)

冈田文彦：“哭泣的倾向是抑郁情绪最有用的指标之一吗？”

Fumihiko Okada: "Reverse placebo effects:anticipation/anxiety at the possibility of receiving placebos." Ann.Pharmacotherapy. 26. 128 (1992)

Fumihiko Okada：“反向安慰剂效应：对接受安慰剂的可能性的预期/焦虑。”

Fumihiko Okada: "Reverse placebo effects:anticipation/anxiety at the possibility of receiving placebos." Ann.Pharmacotherapy. 26. 128- (1992)

Fumihiko Okada：“反向安慰剂效应：对接受安慰剂的可能性的预期/焦虑。”

Fumihiko Okada;Timothy J.Crow;Gareth W.Roberts: "G proteins(Gi,Go)in the medial temporal lobe in schizophrenia:preliminary report of a neurochemical correlate of structural change." J.Neural Transm.84. 147-153 (1991)

Fumihiko Okada；Timothy J.Crow；Gareth W.Roberts：“精神分裂症内侧颞叶中的 G 蛋白（Gi，Go）：与结构变化相关的神经化学的初步报告。”

Fumihiko Okada;Toshimitu Aida;Hiroshi Abe: "Schizophrenic symptoms induced by a tumor of the left basal ganglia with ipsilateral cerebral hemiatrophy." Ann.Clin.Psychiatry. (1992)

Fumihiko Okada；Toshimitu Aida；Hiroshi Abe：“左侧基底神经节肿瘤伴同侧大脑偏侧萎缩引起的精神分裂症症状。”