Pathophysiology and treatment of liver cancer -Cell biology of liver cancer and tumor-bearing liver and its clinical application-

从细胞生长机制和生长调控机制的角度对肝癌和荷瘤肝的细胞生物学进行了研究，从而阐明了肿瘤发生和复发的机制。然后对刑事申请进行评估。本研究分为三个主要项目1。生长因子对肝癌及荷瘤肝影响的分析。大鼠肝大部切除后肝再生与肝细胞癌细胞的动态变化：提示肝大部切除诱导的某些生长因子(S)可能作用于肝细胞，肝癌细胞分泌的其他生长因子可能作用于肝大部切除后再生的肝细胞。自发性肝炎和肝癌大鼠肝细胞对生长因子敏感性降低：提示自发性肝炎和肝癌大鼠肝细胞对生长因子敏感性降低可能促进f-…的发生。更多的暴发性肝炎，并为启动细胞进行选择性生长提供了机会。肝癌及荷瘤肝细胞核DNA含量及生长因子受体分析。自然发展为肝炎和肝癌的LEC大鼠肝细胞的核DNA倍体分析：根据结果，我们推测高铜蓄积引起的肝细胞的持续坏死和再生导致了LEC大鼠的癌变。肝细胞生长因子受体在肝细胞再生和癌变中的作用分析：结果提示甲硫氨酸前受体的加工与肝脏的癌变、分化和再生密切相关。肝癌和荷瘤肝细胞生物学的临床应用。新出现的伴有肝损害的血清组份的诊断意义：高效液相色谱法洗脱的峰-1血清组份作为肝功能衰竭的预测标志物可能具有诊断意义。肝切除患者手术前后人肝细胞生长因子水平的变化：结果表明，血清肝细胞生长因子水平与肝储备功能及术后形态再生有关。肝母细胞瘤细胞核DNA含量的流式细胞术分析结果提示，核DNA分析可能有助于肝母细胞瘤的预后判断。D.应用核DNA分析预测人肝癌切除后复发：在肝细胞癌复发病例中，肿瘤核DNA倍体模式被认为是反映其恶性潜能的有用因素。较少

The cell biology of liver cancer and tumor-bearing liver was studied from the point of view of the cell growth mechanism and growth regulation mechanism, thereby allowing us to clarify the mechanism of carcinogenesis and tumor recurrence. Then criminal applications were evaluated. This research has been divided into three major projects.1. Analysis of the effect of growth factors on liver cancer and tumor-bearing livera. Kinetic changes of liver regeneration and hepatocellular carcinoma(HCC) cell after partial hepatectomy in rats: The results indicated that some growth factors(s) induced by partial hepatectomy may act on HCC cells, and that other growth factors secreted by HCC cells may act on the regenerating hepatocytes after partial hepatectomy.b. Reduced susceptibilities to growth factors of hepatocytes isolated from LEC rats with spontaneous hepatitis and hepatoma: The results suggested that reduction of susceptibility to growth stimuli in LEC rats may promote the development of f … More ulminant hepatitis and provide opportunities for initiated cells to undergo selective growth.2. Analysis of the nuclear DNA contents and the growth factor receptor of liver cancer and tumor-bearing livera. Nuclear DNA ploidy analysis of liver cells in LEC rats naturally developing hepatitis and hepatoma: From the results, we speculated that continuous necrosis and regeneration of the hepatocytes caused by high accumulation of copper results in carcinogenesis in LEC rats.b. Analysis of the hepatocyte growth factor receptor in regeneration and oncogenesis of hepatocytes: The results suggested that processing of the Met proreceptor is closely associated with carcinogenesis, its differentiation and also regeneration of the liver.3. Clinical applications of cell biology of liver cancer and tumor-bearing livera. Diagnostic significance of newly appeared serum fractions accompanied by liver damage: The peak-1 serum fraction eluted by high performance liquid chromatography(HPLC) may have diagnostic significance as a predictable marker of hepatic failure.b. Preoperative and postoperative variations of human hepatocyte growth factor(hHGF) in hepatectomized patients: the results indicated that serum hHGF level correlated with hepatic functional reserve and postoperative morphological regeneration.c. Flow cytometric Analysis of the nuclear DNA content of hepatoblastema: The results indicated that nuclear DNA analysis might be useful in investigating the prognosis of hepatoblastema. d. Prediction of recurrence after resection in human hepatomas by nuclear DNA analysis: In recurrent cases of HCC, it was considered that the tumor nuclear DNA ploidy pattern is a useful factor to indicate the potential of malignancy. Less