Pathophysiology and Treatment of Liver Cancer - The Effects of Liver Regeneration Factors

本文将阐明肝营养因子对肝癌肿瘤生长的影响，并对新的治疗方法的发展进行评价。这项研究分为三个项目。部分肝切除后肝再生大鼠肝癌生长：部分肝切除后大鼠肿瘤生长增强。这在肝切除术后24小时内最为明显。肿瘤细胞有丝分裂指数的增加证实了这一事实。肝部分切除术促进肝癌生长的作用机制分析：通过对肿瘤细胞免疫、雌激素受体及肝营养因子的检测，分析其作用机制。术后免疫（NK活性和母细胞发生）的变化与肝癌生长的变化无关。在所有标本中均未检测到肝癌大鼠雌激素受体。取肝部分切除后24小时的大鼠血清，用高效液相色谱法洗脱成50份。原代培养的肝细胞和肿瘤细胞均仅增强了30分之一（100 Kd）。人表皮生长因子（hEGF）对抗肿瘤药物抗肿瘤作用的增强作用：测定4组肝癌（HC-4）移植裸鼠的生长曲线；对照组、hEGF组、γ干扰素（γ INF）组和hEGF+gammaINF组。在hEGF+gammaINF组中，生长抑制似乎是最重要的。综上所述，本研究阐明了肝切除术后肿瘤生长增强及肿瘤形成机制，为肝癌治疗开辟了新的方法。

The Effects of hepatotrophic factors on the tumor growth of liver cancer will be clarified and the development of new therpeutic methods will be evaluated. This research has been devided into three projects.1. Growth of liver cancer in rats concomitant with liver regeneration following partial hepatectomy: Tumor growth was enhanced following partial hepatectomy in rats. This was most obvious within 24 hours following hepatectomy. This fact was substantiated by the increase in mitotic indices of the tumor cells.2. Analysis of the mechanism of the effect of partial hepatectomy on enhanced growth of liver cancer: To analyse the mechanism of the previous results the immunity, the esrogen receptor of the tumor cells and the hepatotrophic factors were examined. The changes in postoperative immunity(NK activity and blastgenesis) did not correlate with the changes in liver cancer growth. Estrogen receptor of liver cancer in rats was not detected in any specimen. Serum obtained from rats 24 hours after partial hepatectomy was eluted into 50 fractions by high performance liquid chromatography. Primarily cultivated hepatocytes and tumor cells were both enhanced only by a 30th fraction(100 Kd).3. Intensifying effect of human epidermal growth factor (hEGF) on the antitumor action of antitumor agents: The growth curve of hepatocarcinoma(HC-4) transplanted into nude mice was measured in 4 groups; the control group, the hEGF group, the gamma interferon (gamma INF) group and the hEGF+gammaINF group. In the hEGF+gammaINF group, growth inhibition seems to be most important.In conclusion, the enhanced tumor growth following hepatectomy and the tumor mechanism were clarified and new methods of liver cancer treatment were developed through this research.