The study of using cultured fibroblast cells to the preiodontal tissue regeneration.

尤其是牙周韧带来源的成纤维细胞对牙槽骨、牙骨质和牙周膜的再生具有重要作用。本研究从狗的外牙面提取牙周韧带细胞，体外连续培养4~6次后，用美国Zynaxis公司的荧光标记试剂盒对成纤维细胞染色。成纤维细胞样细胞染色良好。染色的细胞进一步培养，推测这些细胞的分裂核几乎与原始细胞染色相同。这些细胞放置在同一狗分叉区的缺损处。从前庭侧行FLAP手术以保持上皮附着。植入的胶原凝胶本身的骨量高于对照组。成纤维细胞样细胞混合在胶原胶中的骨量高于胶原凝胶本身。

It's necessary to keep more over absolute number of cells for the regeneration of periodontally destructed defects.Especialy, fibroblast like cells sourced from periodontal ligaments have important part for the regeneration of alveolar bone, cementum and periodontal ligament.In this study, it had taked the periodontal ligament cells from extraeted teeth surface of the dog.The fibloblast like cells were dyed by fluorescent marker kit (Zynaxis, USA) after 4-6 successive cultured in vitro. The fibroblast like cells were well dyed.The cells that dyed had been cultured futher ore, it was assumed that devided nucleus of these cells were almost the same dyed as originals.These cells were in placed the defects of the furcation area of the same dog.Flap procedure were made from vestiblular side to keep epithelial attachment.The bone of implanted collagen gel by itself was higher than control.Fibroblast like cells mixed into collagen gel was higher than collagen gel by itself.