Cytotoxic Effects of Periodontopathic Bacteria on Human Gingival Epithelial Cell Cultured in Serum-Free Medium.

牙周病细菌对无血清培养基中培养的人牙龈上皮细胞的细胞毒性作用。

• 批准号: 02670849
• 负责人: IZUMI Yuichi
• 金额: $ 1.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670849/
• 关键词: Culture in Serum Free Medium; Gingival Keratinocyte; Periodontal Pathogens; Cell Proliferation; Protein Synthesis; Polymorphonuclear Leukocyte; Lysosomal Enzyme; Cell Detachment; 歯肉上皮細胞; 生物学的活性

The purpose of this investigation was to study the effects of selected periodontal pathogens on cell proliferation, protein synthesis and cell injury in vitro.The ultrasonic extracts from microorganisms included A. actinomycetemcomitans, P. gingivalis, P. intermedia, C. sputigena, E. corrodens, F. nucleatum, A. viscosus and St. mutans. Target cells were keratinocytes SCC9 and gingival keratinocytes cultured in serum-free medium. The results showed that cell proliferation and rate of protein synthesis by SCC9 were strongly inhibited by E. corrodens ; at similar concentrations A. actinomycetemcomitans, P. gingivalis and C. sputigena also significantly reduced the proliferation and synthesis whereas P. intez-media, F. nucleatum, A. viscosus and St. mutans had only minor ef f ects. These results indicate that keratinocyte function may be modulated by periodontopathic bacteria.The cell detachment assay was subjected by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from A … More . actinomycetemcomitans, B. forsythus, F. nucleatum, C. sputigena and E. corrodens. The bacterial extracts alone caused no cell disruption of the keratinocyte monolayers. Polymorphonuclear leukocyte alone caused only a minimal cell detachment. On the contrary, when the bacterial extracts were added to the co-culture of keratinocytes and polymorphonuclear leukocytes, cell detachment of keratinocytes was observed except for the bacterial extract f rom E. corrodens. The effect of A. actinomycetemcomitans was strongest. This effect was heat labile and not inhibited by polymyxin B. Cell detachment was inhibited by alpha 1-antitrypsin but not by catalase and superoxide dismutase. Hydrogen peroxide and leukocyte elastase also caused keratinocyte detachment, and keratinocyte lysis was not observed. These results indicate that polymorphonuclear leukocyte can cause non-lytic cell detachment of the gingival keratinocytes when encountering some bacteria, which may lead to the increase of the permeability of the keratinocyte layers. Less

本研究旨在探讨牙周致病菌超声提取液对体外培养的牙周组织细胞增殖、蛋白质合成及细胞损伤的影响。放线菌共生菌、牙龈卟啉单胞菌、中间卟啉单胞菌、C. sputigena、棘孢E. corrodens，F.核质，A.粘性链球菌和变形链球菌。靶细胞为角质形成细胞SCC 9和无血清培养基中培养的牙龈角质形成细胞。结果表明，E.在相似的浓度下，A. actinomycetemcomitans、P. gingivalis和C. sputigena也显著降低了细胞的增殖和合成，而P. intez-media、F.核质，A.粘性链球菌和变形链球菌仅具有较小的作用。结果表明，牙周致病菌可能对角质形成细胞的功能有调节作用。 ...更多信息 . actinomycetemcomitans、伴放线菌B. Pasthus，F. nucleatum、具核隐翅虫C. sputigena和E.腐蚀单独的细菌提取物不引起角质形成细胞单层的细胞破坏。单纯多形性白细胞仅引起极轻微的细胞脱离。相反，当将细菌提取物加入到角质形成细胞和多形核白细胞的共培养物中时，除了来自E.腐蚀A.放线菌共生菌最强。这种作用是热不稳定的，不受多粘菌素B的抑制。α 1-抗胰蛋白酶，但不是由过氧化氢酶和超氧化物歧化酶抑制细胞脱落。过氧化氢和白细胞弹性蛋白酶也引起角质形成细胞脱落，角质形成细胞溶解没有观察到。这些结果表明，当遇到某些细菌时，多形核白细胞可以引起牙龈角质形成细胞的非溶解性细胞脱离，这可能导致角质形成细胞层的渗透性增加。少

项目成果

Yuichi IZUMI: "Cytotoxic Effects of Periodontopathic Bacterial Extracts on Human Oral Keratinocytes in Culture." The Japanese Journal of Conservative Dentistry. 35. (1992)

Yuichi IZUMI：“牙周病细菌提取物对培养的人口腔角质形成细胞的细胞毒性作用。”

和泉 雄一: "培養口腔角化細胞の機能に対する各種歯周病原性細菌の影響" 日本歯科保存学会雑誌. 35. (1992)

Yuichi Izumi：“各种牙周病原菌对培养的口腔角质细胞功能的影响”日本保守牙科学会杂志 35。（1992）

Makoto UMEDA: "Clinical, Bacteriological and Immunological Examination and Treatment of Two Papillon-Lefevre Syndrome Patients." The Journal of The Stomatological Society. 57-No. 3. 430-440 (1990)

Makoto UMEDA：“两名 Papillon-Lefevre 综合征患者的临床、细菌学和免疫学检查及治疗。”

和泉 雄一: "培養歯肉上皮細胞の機能に対する各種歯周病原性細菌の影響" 口腔病学会雑誌. 第57巻. 451-451 (1990)

Yuichi Izumi：“各种牙周病原菌对培养的牙龈上皮细胞功能的影响”口腔医学会杂志第 57 卷 451-451（1990 年）。

杉山 栄一: "歯周疾患関連細菌抽出物の多形核白血球を介した口腔角化細胞に対する作用。" 口腔病学会雑誌. 59. 13-25 (1992)

Eiichi Sugiyama：“牙周病相关细菌提取物对多形核白细胞介导的口腔角质形成细胞的作用”，日本口腔医学会杂志 59. 13-25 (1992)。