Cytotoxic Effects of Periodontopathic Bacteria on Human Gingival Epithelial Cell Cultured in Serum-Free Medium.

本研究旨在探讨牙周致病菌超声提取液对体外培养的牙周组织细胞增殖、蛋白质合成及细胞损伤的影响。放线菌共生菌、牙龈卟啉单胞菌、中间卟啉单胞菌、C. sputigena、棘孢E. corrodens，F. nucleatum、具核A.粘性链球菌和变形链球菌。靶细胞为角质形成细胞SCC 9和无血清培养基中培养的牙龈角质形成细胞。结果表明，E.在相似的浓度下，A. actinomycetemcomitans、P. gingivalis和C. sputigena也显著降低了细胞的增殖和合成，而P. intez-media、F. nucleatum、具核A.粘性链球菌和变形链球菌仅具有较小的作用。结果表明，牙周致病菌可能对角质形成细胞的功能有调节作用。 ...更多信息 . actinomycetemcomitans、伴放线菌B. Pasthus，F. nucleatum、具核隐翅虫C. sputigena和E.腐蚀单独的细菌提取物不引起角质形成细胞单层的细胞破坏。单纯多形性白细胞仅引起极轻微的细胞脱离。相反，当将细菌提取物加入到角质形成细胞和多形核白细胞的共培养物中时，除了来自E.腐蚀A.放线菌共生菌最强。这种作用是热不稳定的，不受多粘菌素B的抑制。α 1-抗胰蛋白酶，但不是由过氧化氢酶和超氧化物歧化酶抑制细胞脱落。过氧化氢和白细胞弹性蛋白酶也引起角质形成细胞脱落，角质形成细胞溶解没有观察到。这些结果表明，当遇到某些细菌时，多形核白细胞可以引起牙龈角质形成细胞的非溶解性细胞脱离，这可能导致角质形成细胞层的渗透性增加。少

The purpose of this investigation was to study the effects of selected periodontal pathogens on cell proliferation, protein synthesis and cell injury in vitro.The ultrasonic extracts from microorganisms included A. actinomycetemcomitans, P. gingivalis, P. intermedia, C. sputigena, E. corrodens, F. nucleatum, A. viscosus and St. mutans. Target cells were keratinocytes SCC9 and gingival keratinocytes cultured in serum-free medium. The results showed that cell proliferation and rate of protein synthesis by SCC9 were strongly inhibited by E. corrodens ; at similar concentrations A. actinomycetemcomitans, P. gingivalis and C. sputigena also significantly reduced the proliferation and synthesis whereas P. intez-media, F. nucleatum, A. viscosus and St. mutans had only minor ef f ects. These results indicate that keratinocyte function may be modulated by periodontopathic bacteria.The cell detachment assay was subjected by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from A … More . actinomycetemcomitans, B. forsythus, F. nucleatum, C. sputigena and E. corrodens. The bacterial extracts alone caused no cell disruption of the keratinocyte monolayers. Polymorphonuclear leukocyte alone caused only a minimal cell detachment. On the contrary, when the bacterial extracts were added to the co-culture of keratinocytes and polymorphonuclear leukocytes, cell detachment of keratinocytes was observed except for the bacterial extract f rom E. corrodens. The effect of A. actinomycetemcomitans was strongest. This effect was heat labile and not inhibited by polymyxin B. Cell detachment was inhibited by alpha 1-antitrypsin but not by catalase and superoxide dismutase. Hydrogen peroxide and leukocyte elastase also caused keratinocyte detachment, and keratinocyte lysis was not observed. These results indicate that polymorphonuclear leukocyte can cause non-lytic cell detachment of the gingival keratinocytes when encountering some bacteria, which may lead to the increase of the permeability of the keratinocyte layers. Less