An analysis on oncogene mutations in oral leukoplakia with PCR-SSCP

PCR-SSCP分析口腔白斑癌基因突变

• 批准号: 03454465
• 负责人: ISHIDA Takeshi
• 金额: $ 4.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454465/
• 关键词: Tongue; Squamous cell carcinoma; Leukoplakia; p53; Gene mutations; DNA増幅; PCR

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.

p53肿瘤抑制基因在口服鳞状细胞癌癌中的作用尚不清楚。这项研究的目的是通过PCR-SSCP研究口腔鳞状细胞癌和白细胞中p53基因突变的频率。模板DNA是从福尔马林固定的，石蜡包含的样品中提取的（通过舌鳞状细胞癌的11例，舌肠癌和tongue leuk leuk kakia和2正常的gingiv gingiviin canse cansemase casimos casemote gote kyemanted sscp。在每个样品上用4个底漆对外显子5,6,7和8个p53基因进行PCR。 PCR产物在天然梯度聚丙烯酰胺凝胶中电泳，并通过银染色（PCR-SSCP）检测。在1例鳞状细胞癌和1例白细胞中检测到突变存在的额外条带。