An analysis on oncogene mutations in oral leukoplakia with PCR-SSCP

PCR-SSCP分析口腔白斑癌基因突变

• 批准号: 03454465
• 负责人: ISHIDA Takeshi
• 金额: $ 4.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454465/
• 关键词: Tongue; Squamous cell carcinoma; Leukoplakia; p53; Gene mutations; DNA増幅; PCR

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.

p53抑癌基因在口腔鳞癌发生发展中的作用尚不清楚。本研究采用PCR-SSCP方法检测11例舌鳞癌、9例舌白斑和2例正常牙龈组织中p53基因的突变频率。用p53基因第5、6、7、8外显子的4对引物进行PCR扩增。PCR产物经聚丙烯酰胺凝胶电泳分离后，进行银染（PCR-SSCP）检测。在1例鳞状细胞癌和1例白斑中检测到表明存在突变的额外条带。