An analysis on oncogene mutations in oral leukoplakia with PCR-SSCP

P53抑癌基因在口腔鳞状细胞癌发生中的作用尚不清楚。应用聚合酶链式反应-单链构象多态方法检测口腔鳞状细胞癌和白斑组织中p53基因突变的频率。用4对P53基因第5、6、7、8外显子对每个样本进行聚合酶链式反应。聚合酶链式反应产物经自然梯度聚丙烯酰胺凝胶电泳法和银染法进行检测。在1例鳞状细胞癌和1例白斑中检测到额外的突变条带。

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.