PCR-SSCP分析口腔白斑癌基因突变-猫眼课题宝

PCR-SSCP分析口腔白斑癌基因突变

批准号：

03454465

负责人：

ISHIDA Takeshi

金额：

$4.22万

依托单位：

Osaka University

依托单位国家：

日本

项目类别：

Grant-in-Aid for General Scientific Research (B)

财政年份：

1991

资助国家：

日本

项目状态：

已结题

起止时间：

1991 至 1993

来源：

https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454465/

关键词：

Tongue Squamous cell carcinoma Leukoplakia p53 Gene mutations DNA増幅 PCR

项目摘要

项目成果

ISHIDA Takeshi的其他基金

相似基金

相关文献

中文摘要

翻译

点击翻译按钮获取中文摘要

英文摘要

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.

期刊论文(0)

专著(0)

科研奖励(0)

会议论文