Statistical Analysis of Histopathological Parameters for Assessing Prognosis of Oral Squamous Cell Carcinoma Cases

用于评估口腔鳞状细胞癌病例预后的组织病理学参数的统计分析

基本信息

批准号：
62570898
负责人：
ISHIDA Takeshi
金额：
$ 1.22万
依托单位：
Hospital attached to the Osaka University Faculty of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

DNA ploidy analysis of formalin-fixed, paraffin embedded materials of 10 cases of oral squamous cell carcinoma(SCC) was performed by two different methods, microspectrophotmetry with 5 micron sections and cytofluorometry with isolated tumor cells to choose the method for the biopsy materials. There were few cases which showed same DNA histogram pattern in two methods. This was due to the insufficient accuracy in measurement of single cell DNA content with the microspectrophotometric method.Twenty-five cases with the tongue SCC,which had recieved radiation therapy and had sufficient clinical and follow-up data, were available for this study. Isolated tumor cells from the paraffinembedded materials were stained with DADI and DNA histogram was made of nuclear DNA content of 50-100 tumor cells. According to the criteria of Auer et al., DNA polidy patterns were divided into 4 types. Type I with a single diploid (2C)peak and type II with a diploid(2C) and/or a tetraploid(4C)peak were summarized as low ploidy group. Type III with a sizable amount of cells with DNA content between 2C and 4C representing proliferating cells and type IV showing a broad DNA distribution ranging from 2C to beyond 8C were summarized as high ploidy group. Survival curves (Kaplan-Meier method) of two groups demonstrated the more unfavorable prognosis in high ploidy cases, however, the number of analysed case was not sufficient so that the difference between two survival curves was not statistically significant with logrank test or Cox-Mantel test. Death was more frequently encountered in the high ploidy cases(9/14) than in the low ploidy cases(4/11).

通过两种不同的方法，具有5个微米切片的微光照射法和细胞荧光测定法，用隔离的肿瘤细胞使用隔离的肿瘤细胞，对10例口腔鳞状细胞癌（SCC）进行了10例口腔鳞状细胞癌（SCC）的孔蛋白固定的嵌入式材料的DNA倍性分析。在两种方法中，很少有情况显示出相同的DNA直方图模式。这是由于使用微光谱测量方法在测量单细胞DNA含量方面的准确性不足所致。有舌SCC的二十五例接受放射治疗，并具有足够的临床和随访数据，可用于本研究。用DADI染色了从石蜡膜材料中分离出的肿瘤细胞，DNA直方图由50-100个肿瘤细胞的核DNA含量制成。根据Auer等人的标准，将DNA polidy模式分为4种类型。 I型具有单二倍体（2C）峰和II型I II型，二倍体（2C）和/或四倍体（4C）峰的I总结为低倍性基团。 III型具有相当数量的细胞，在2C和4C之间具有DNA含量，代表增殖细胞和IV型，显示了从2C到8c以上的宽DNA分布，总结为高倍性基团。两组的生存曲线（Kaplan-Meier方法）表明，在高倍性病例中的预后更不良，但是，分析病例的数量不够，因此在Logrank Test或Cox-Mantel检验中，两个生存曲线之间的差异在统计学上没有统计学意义。在高倍性病例（9/14）中，死亡经常遇到的频率比低倍性病例（4/11）更常见。