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Statistical Analysis of Histopathological Parameters for Assessing Prognosis of Oral Squamous Cell Carcinoma Cases

用于评估口腔鳞状细胞癌病例预后的组织病理学参数的统计分析

基本信息

批准号：
62570898
负责人：
ISHIDA Takeshi
金额：
$ 1.22万
依托单位：
Hospital attached to the Osaka University Faculty of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

DNA ploidy analysis of formalin-fixed, paraffin embedded materials of 10 cases of oral squamous cell carcinoma(SCC) was performed by two different methods, microspectrophotmetry with 5 micron sections and cytofluorometry with isolated tumor cells to choose the method for the biopsy materials. There were few cases which showed same DNA histogram pattern in two methods. This was due to the insufficient accuracy in measurement of single cell DNA content with the microspectrophotometric method.Twenty-five cases with the tongue SCC,which had recieved radiation therapy and had sufficient clinical and follow-up data, were available for this study. Isolated tumor cells from the paraffinembedded materials were stained with DADI and DNA histogram was made of nuclear DNA content of 50-100 tumor cells. According to the criteria of Auer et al., DNA polidy patterns were divided into 4 types. Type I with a single diploid (2C)peak and type II with a diploid(2C) and/or a tetraploid(4C)peak were summarized as low ploidy group. Type III with a sizable amount of cells with DNA content between 2C and 4C representing proliferating cells and type IV showing a broad DNA distribution ranging from 2C to beyond 8C were summarized as high ploidy group. Survival curves (Kaplan-Meier method) of two groups demonstrated the more unfavorable prognosis in high ploidy cases, however, the number of analysed case was not sufficient so that the difference between two survival curves was not statistically significant with logrank test or Cox-Mantel test. Death was more frequently encountered in the high ploidy cases(9/14) than in the low ploidy cases(4/11).

对10例口腔鳞状细胞癌（SCC）的福尔马林固定石蜡包埋材料进行DNA倍性分析，采用5微米切片显微分光光度法和离体肿瘤细胞荧光法两种不同的方法选择活检材料。两种方法的DNA直方图显示相同的病例很少。这是由于用微量分光光度法测量单细胞DNA含量的准确性不足。本研究收集了25例接受放射治疗并有足够临床和随访资料的舌鳞癌患者。对石蜡包埋材料中分离的肿瘤细胞进行DADI染色，绘制50 ~ 100个肿瘤细胞核DNA含量的DNA直方图。根据Auer等人的标准，将DNA多态性模式分为4种类型。具有单个二倍体（2C）峰的I型和具有二倍体（2C）和/或四倍体（4C）峰的II型被总结为低倍性组。DNA含量在2C到4C之间的细胞数量较多的III型代表增殖细胞，DNA分布在2C到8C以上的DNA分布较广的IV型代表高倍性群体。两组患者的生存曲线（Kaplan-Meier法）均显示高倍性患者预后较差，但由于分析病例数不足，采用logrank检验或Cox-Mantel检验，两组患者的生存曲线差异无统计学意义。高倍性病例（9/14）的死亡率高于低倍性病例（4/11）。