Radioprotective effect of ADF, ATL-derived factor

ADF、ATL衍生因子的放射防护作用

• 批准号: 03454551
• 负责人: UCHIDA Atsushi
• 金额: $ 0.32万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454551/
• 关键词: ADF; X-rays; NK cell; Bone marrow; 造血幹細胞コロニ-形成; フリ-ラジカル

We have been studying the effects of biological response modifiers (BRM) on the host defense system against stress including X-ray irradiation. ADF, ATL-derived factor, has been shown to have redox potential such as thioredoxin and other various biological activities. In the present study the radioprotective effect of ADF on bone marrow death was examined in the mouse system. When mice were treated with recombinant human ADF immediately after exposure to lethal doses of X-rays, they escaped from acute bone marrow death and survived more than 30 days. Multiple administration of ADF was required for the manifestation of the radioprotective effect. ADF injection prevented the decrease in NK (natural killer) cell activity induced by X-ray irradiation. However, the numbers of granulocytes, platelets and erythrocytes in the peripheral blood and their colony forming units were not affected by ADF. The genetically modified ADF with less reducing activity was less effective. These results indicate that ADF may produce the radioprotective effect through activation of host defense against infection.

我们一直在研究生物反应调节剂（BRM）对宿主防御系统的影响，以应对包括X射线照射在内的应激。ADF，ATL衍生因子，已被证明具有氧化还原电位，如硫氧还蛋白和其他各种生物活性。在本研究中，在小鼠系统中检查ADF对骨髓死亡的辐射保护作用。当小鼠在暴露于致死剂量的X射线后立即用重组人ADF治疗时，它们逃脱了急性骨髓死亡，存活了30多天。需要多次给予ADF以显示辐射防护作用。ADF注射防止了由X射线照射引起的NK（自然杀伤）细胞活性的降低。ADF对外周血中粒细胞、血小板和红细胞的数量及其集落形成单位无明显影响。还原活性较低的转基因ADF效果较差。这些结果表明，ADF可能通过激活宿主对感染的防御机制而产生辐射防护作用。

项目成果

Sorachi K.,Sugie K.,Maekawa N.,Takami M.,Kawabe T.,Yodoi J.: "Induction and function of FceRII on YT cells;Possible role of ADF/Thioredoxin in FceRII expression." Immunobiology. 185. 193-206 (1992)

Sorachi K.、Sugie K.、Maekawa N.、Takami M.、Kawabe T.、Yodoi J.：“FceRII 对 YT 细胞的诱导和功能；ADF/硫氧还蛋白在 FceRII 表达中的可能作用。”

Kurishita a.,Katoh H.,Uchida a.,Ono t.,Hirose S.,Okada S.: "Post-irradiation treatment with OK432 can rescue radiation-induced bone marrow death." Int.J.Radiation Biol.59. 711-716 (1991)

Kurishita a.、Katoh H.、Uchida a.、Ono t.、Hirose S.、Okada S.：“用 OK432 进行辐射后治疗可以挽救辐射引起的骨髓死亡。”

Iwata S.,Matsuda M.,Sugie K.,Maeda Y.,Kawabe T.,Yodoi J.: "Signal transduction via Fc receptors;Involvement of tyrosine kinase and redox regulation by ADF." Redent Advances in Mucosal Immunology.

Iwata S.、Matsuda M.、Sugie K.、Maeda Y.、Kawabe T.、Yodoi J.：“通过 Fc 受体进行信号转导；参与酪氨酸激酶和 ADF 的氧化还原调节。”

Sorachi k.,Sugie K.,Maekawa N.,Takami M.,Kawabe T.,Yodoi j.: "Induction and function of FceRII on YT cells;Possible role of ADF/Thioredoxin in FceRII espression." Immunobiology. 185. 193-206 (1992)

Sorachi k.、Sugie K.、Maekawa N.、Takami M.、Kawabe T.、Yodoi j.：“FceRII 对 YT 细胞的诱导和功能；ADF/硫氧还蛋白在 FceRII 表达中的可能作用。”

Matsuda M.,Masutani H.,Miyajima S.,Uchida A.,Horiuchi A.,Yodoi J.: "Protective activity of ATL-derived factor (ADF) against tumor necrosis factor-dopendent cytotoxicity on U937 cells." J.Immunology. 147. 3837-3841 (1991)

Matsuda M.、Masutani H.、Miyajima S.、Uchida A.、Horiuchi A.、Yodoi J.：“ATL 衍生因子 (ADF) 对 U937 细胞的肿瘤坏死因子依赖性细胞毒性的保护活性。”