In situ observation of protein crystallization under high pressure

1.高压原位观察系统的设计与搭建：为适应冶金相差显微镜的需要，设计了一种金刚石压腔。利用红宝石激光的光谱荧光位移，建立了压力测量系统。发现了一种通过对溶菌酶晶体施加压力来控制核数的可能方法，发现在压力下成核时，溶菌酶的核数减少。压力越高，出现的核数越少。溶菌酶的溶解度与压力的关系：用顶锤在25±0.2ﾟC下将压力从510 Mpa改变到0.1Mpa。结果表明，随着压力的降低，溶菌酶(四方晶系)的单晶逐渐长大。首次明确了溶菌酶的溶解度随着容器内压力的增加而增加。施加压力对溶菌酶生长习性的影响：垂直于(101)面和(110)面的生长率之比随着压力的增加而单调增加。这导致了晶体的习性变化，即大块晶体的c轴。

1. Designing and build-in of the high pressure in situ observation system :A diamond anvil cell was designed to fit the metallurgical phase-contrast microscope. The pressure measurements system was built up using the fluorescence shift of a spectrum from a ruby laser beam.2. A possible way of controlling the number of nuclei by applying pressure on HEW lysozyme crystals was found.It was found that the number of nuclei of HEW lysozyme diminishes when the nucleation took place under pressure. The higher the pressure the less number of nuclei appeared.3. The pressure dependence of the solubility of HEW lysozyme :The pressure was changed from 510MPa to 0.1 MPa at 25(〕SY.+-.〔)0.2ﾟC using the anvil. It was found that the single crystals of HEW lysozyme (tetragonal system) grew as the pressure was decreased. It became clear for the first time that the solubility of HEW lysozyme increased when the pressure in the vessel was increased.4. Habit change of HEW lysozyme by applying pressure :The ratio of growth rate vertical to the faces (101) and (110) increased monotonously as the pressure was increased. This resulted in the habit change of the crystal ; the bulk crystals c axis.