EFFECTS OF CIRCUMSTANCE ON BIO-PRODUCTION

环境对生物生产的影响

基本信息

  • 批准号:
    04453134
  • 负责人:
  • 金额:
    $ 3.71万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

In the field of agriculture and fermentation industry, insoluble substrates and heterogeneous microbes play important roles. In this work, we studied on the multiplicity of enzyme and heterogenicity of microbes that affect on various kind of fermentations.1) Glucoamylase of Aspergillus awamori var. kawachi shows multiplicity on digestivity of raw-starch. GAI that effectively digests raw starch had catalytic site and affinity sites on raw-starch. The affinity sites for raw starch were composed o fCp and GpI, which were binding site on raw starch and promoting site of raw-starch hydration, respectively. The relationships between structure and function of them were studied by site-specific mutagenesis.2) Multiploidity of sake-brewery yeast was developed by using mater diploid-cells, that were induced by heat treatment of spores. On the other hand, it was found that Saccharomyces cerevisiae wy2 showed a novel life cycle, named delayd homothallism. It was found tha the strain had a defect in HO gene.4) The degeneration of Clostridium acetobutylicum observed in the serial subculture were found to be caused by spontaneous mutants. The degenerated strain were defected in NADH generation system, and grew rapidly even in the stationary phase of the wild-type strain.5) A new process for animal-feces treatment was developed by using Coprophilous microbes. The treated feces, that enhance the growthof fluorescent pseudomonads or non-pathogenic fungi, could depress the incidence of potato common scab.
在农业和发酵工业领域,不溶性底物和异质微生物起着重要的作用。本文研究了影响各种发酵过程的酶的多样性和微生物的异质性。1)川曲霉葡萄糖淀粉酶对生淀粉的消化率具有多样性。能有效消化生淀粉的GAI对生淀粉有催化位点和亲和位点。对原料淀粉的亲和位点由fCp和GpI组成,分别是原料淀粉的结合位点和原料淀粉水化促进位点。通过位点特异性诱变研究了它们的结构与功能之间的关系。2)利用对孢子进行热处理诱导的母质二倍体细胞,获得了清酿酒酵母的多倍性。另一方面,发现酿酒酵母wy2表现出一种新的生命周期,称为延迟同殖现象。结果发现该菌株存在HO基因缺陷。4)在连续传代培养中观察到的乙酰丁酸梭菌的退化是由自发突变引起的。退化菌株在NADH生成系统中存在缺陷,即使在野生型菌株的静止期也能快速生长。5)研究了利用嗜粪菌处理动物粪便的新工艺。处理后的粪便能促进荧光假单胞菌或非致病性真菌的生长,从而降低马铃薯普通痂的发病率。

项目成果

期刊论文数量(62)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Goto, K.Furukawa, and S.Hayashida: "An Avicel-affinity site in an Avicel-digesting exocellulase from a Trichoderma viride Mutant" Biosci. Biotech. Biochem.56. 1523-1528 (1992)
M.Goto、K.Furukawa 和 S.Hayashida:“来自绿色木霉突变体的 Avicel 消化外切纤维素酶中的 Avicel 亲和位点”Biosci。
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    0
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S.Hayashida, M.Goto: "The mechanism for digestion of the crystalline carbohydrates by amylase and cellualase on the basis for the theory of the affinity site" Sci. Bull. Fac. Agr. Kyushu Univ.47. 73-83 (1992)
S.Hayashida,M.Goto:“基于亲和位点理论的淀粉酶和纤维素酶消化结晶碳水化合物的机制”Sci。
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    0
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Y.Tani, Y.Tomohiro, A.Miyata, K.Furukawa, S.Hayashida: "Endomitotic diploidization of Saccharomyces cerevisiae by heat treatment during spore germination" Yeast. 9. 519-521 (1993)
Y.Tani、Y.Tomohiro、A.Miyata、K.Furukawa、S.Hayashida:“孢子萌发过程中通过热处理实现酿酒酵母的内丝分裂二倍体”酵母。
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    0
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Hayashida,S.: "Specific Inhibition by cyclodextrins of raw starch digestion by fungal glucoamylase" Biosci.Bitech.Biochem.56. 556-559 (1992)
Hayashida,S.:“环糊精对真菌葡糖淀粉酶消化生淀粉的特异性抑制”Biosci.Bitech.Biochem.56。
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    0
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Hayashida,S.: "The mechanism of binding of glucoamylase I from Aspergillus awamori var.kawachi to cyclodextrins and raw starch" Biosci.Biotech.Biochem.58. 49-54 (1994)
Hayashida,S.:“泡盛曲霉河内变种的葡糖淀粉酶 I 与环糊精和生淀粉的结合机制”Biosci.Biotech.Biochem.58。
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    0
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HAYASHIDA Shinsaku其他文献

HAYASHIDA Shinsaku的其他文献

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{{ truncateString('HAYASHIDA Shinsaku', 18)}}的其他基金

Production and Mode of Action of Novel Endoglucanase Disintegrating Native Cellulose
新型内切葡聚糖酶崩解天然纤维素的生产及其作用方式
  • 批准号:
    61560126
  • 财政年份:
    1986
  • 资助金额:
    $ 3.71万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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    2020
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Mascoma's Glucoamylase(GA) producing yeast
Mascoma 生产葡糖淀粉酶 (GA) 的酵母
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    466697-2014
  • 财政年份:
    2014
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    $ 3.71万
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    Experience Awards (previously Industrial Undergraduate Student Research Awards)
FUNGAL GLUCOAMYLASE SUPPLEMENTS ON STARCH DIGESTION IN GLUCOSIDASE DEFICIENT
真菌葡糖淀粉酶补充葡萄糖苷酶缺乏症中的淀粉消化
  • 批准号:
    8356720
  • 财政年份:
    2010
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Structural investigation into the complementary roles of human maltase-glucoamylase and sucrase-isomaltase in starch digestion.
人麦芽糖酶-葡糖淀粉酶和蔗糖酶-异麦芽酶在淀粉消化中互补作用的结构研究。
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    348519-2007
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    2009
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    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
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麦芽糖酶-葡糖淀粉酶:淀粉消化的调节剂
  • 批准号:
    8166647
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Structural investigation into the complementary roles of human maltase-glucoamylase and sucrase-isomaltase in starch digestion.
人麦芽糖酶-葡糖淀粉酶和蔗糖酶-异麦芽酶在淀粉消化中互补作用的结构研究。
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    348519-2007
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    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
MALTASE-GLUCOAMYLASE: REGULATOR OF STARCH DIGESTION
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  • 批准号:
    7950580
  • 财政年份:
    2008
  • 资助金额:
    $ 3.71万
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    7605835
  • 财政年份:
    2007
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    $ 3.71万
  • 项目类别:
Structural investigation into the complementary roles of human maltase-glucoamylase and sucrase-isomaltase in starch digestion.
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    348519-2007
  • 财政年份:
    2007
  • 资助金额:
    $ 3.71万
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    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
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