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Studies of tissue culture on some species of Laminariales (Phaeophyta)

海带目（褐藻门）某些物种的组织培养研究

基本信息

批准号：
04454092
负责人：
NOTOYA Masahiro
金额：
$ 3.46万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454092/
关键词：
Tissue culture Laminariales Phaeophyta

项目摘要

In this research, to observe the influence of lght intensity and temperaure on the callus development and differentiation from blade pieces (about 0.5 x0.5 mm) exciesed from young sporophytes (about 5 mm in blade length) ofseveral species of Laminariales.Agarum cribrosum, Alaria crassiforia, costaria costata, eckolnia cava, E.kurome, E.stolonifera, Eckloniopsis radicosa, Eisenia bicyclis, Laminaria japonica, Streptophyllopsis kuroshioensis, Undaria pinnatifida and U.undarioides were used in the experiment. Callus development and differentiation was examined under various temperatures (10, 15, 20, 25ﾟC) and light intensities (10, 20, 40, 80 mu mol m^<-2>s^<-1>) with a photoperiod of 14L : 10D for 32 days.Callus cells were produced on the cut edge of explants after four days. These primodial callus cells were hemispherical in eight species of A.cribrosum, C.costata, E.cava, E.kurome, E.stolonifera, Ec. radicosa, Ei. bicyclis, L.japonica, except for four species of A.crassiforia, S.kurosh … More ioensis, U.pinnatifida, and U.undarioides in which they were club-shaped. The callus development occurred in nine species except for A.crassiforia, S.kuroshioensis, and U.undarioides. Plastids in developed callus cells were smaller than those in original blade cells. The callus development was favorable under lower light intensities and lower temperatures ; 10ﾟCat all light intensities examined in A.cribrosum, 15ﾟC at 10-20 mu mol m^<-2>s^<-1>, in C.costata, E.kurome and E.stolenifera, 20ﾟC at 10-20 mu mol m^<-2>s^<-1>, in E.cova, 15ﾟC at 20 mu mol m^<-2>s^<-1>, in Ec. radicosa, 20ﾟC at 10 mu mol m^<-2>s^<-1>, in Ei. bicyclis and 15ﾟC at 10 mu mol m^<-2>s^<-1>, in U.pinnatifida. The differentiation of callus cells into blade occurred in seven species except for A.cribrosum and E.kurome. The earliest differentiation of callus cells into blade ocurred at higher temperatures and higher light intensities ; 20ﾟC at 80 mu mol m^<-2>s^<-1>, inC.costata, L.japponica and U.pinnatifida, 25ﾟC at 80 mu mol m^<-2>s^<-1>, in E.cava, E.stolonifera, Ec. radicosa and Ei. bicyclis. Less

本研究观察了光照强度和温度对几种层压藻幼芽孢体（叶片长约5mm）叶片片（约0.5 x0.5 mm）愈伤组织发育和分化的影响。以沙棘木耳、粗叶木耳、海参、紫斑木耳、黑金木耳、黑金木耳、根茎木耳、双环爱森木耳、日本海带木耳、黑盐链木耳、裙带菜木耳和黑金木耳木耳为试验材料。在14L: 10D的光照条件下，分别在10、15、20、25个温度和10、20、40、80 μ mol m^<-2>s^<-1>的光强条件下观察愈伤组织的发育和分化。4天后，外植体的边缘产生愈伤组织细胞。这些原始愈伤组织细胞是半球形的，在8个品种中都有。radicosa, Ei。除A.crassiforia、S.kurosh、More ioensis、U.pinnatifida和U.undarioides为棍棒状外，其余4种呈棍棒状。除A.crassiforia、S.kuroshioensis和uundarioides外，其余9种均有愈伤组织发育。发育后愈伤组织细胞的质体比原始叶片细胞的质体小。较低光强和较低温度有利于愈伤组织发育；10 .在黄花蒿中，15 C在10-20 μ mol m^<-2 bbbs ^<-1>，在黄花蒿、黄花蒿和黄花蒿中，20 C在10-20 μ mol m^<-2>s^<-1>，在黄花蒿中，15 C在20 μ mol m^<-2>s^<-1>，在黄花蒿中。在10 μ mol时，20 μ C <-2>s^<-1>, in Ei。在10 μ mol m^<-2>s^<-1>时，在山裙菜中产生了双环双环和15个甲基苯甲酸。除沙棘和黑马外，其余七种植物的愈伤组织细胞均分化为叶片。在较高的温度和较高的光照条件下，愈伤组织最早向叶片分化；20 .在80 μ mol μ m^<-2 bbb s^<-1>， C.costata， L.japponica， U.pinnatifida; 25 .在80 μ mol μ m^<-2>s^<-1>， E.cava， E.stolonifera，等。根茎和叶黄素。bicyclis。少