A selective labelling of the olfactory neuron by retrovirus with the foreigen genes and contral projection of the labelled olfactory neuron.

将pZip-Neo-CAT（氯代乙酰转移酶）载体转化到缺乏包装信号的pzip -细胞系中。用G418（尼霉素类似物）分离得到CAT基因的psi^-细胞株，并检测其CAT活性。建立了一株产病毒细胞系（8-7）。通过培养产生病毒的细胞获得带有外源基因的病毒。使用成年大鼠。在麻醉下，嗅球被移除。在嗅球切除（OBx）后4天，用cat引入的病毒转染动物。病毒转染一周后，用4%多聚甲醛固定动物。切开冰冻的前部切片。切片用抗CAT抗体孵育。OBx大鼠嗅觉感觉神经元未见cat阳性。产病毒细胞系的滴度可能不够高或病毒环境基因的侵染力过弱。

A pZip-Neo-CAT(chlormaphenical acetyl transferase) vector was transformed into a psi^- cell line which lacked a packaging signal. The psi^- cell line with the CAT gene was segregated by G418 (neamycin analogue) and its CAT activity was checked. A virus producing cell line (8-7) was established. Viruses with the foreigen genes were harvested by culturing the virus producig cells.The adult rats were used. Under anesthesia the olfactory bulb was removed. In four days after olfactory bulbectomy (OBx), animals were transfected with the CAT-introduced viruses. One week after viral transfection, animals were fixed with 4% paraformaldehyde. Frozen flontal sections were cut. The sections were incubated with an antibody against CAT.CAT-positive olfactory sensory neurons were not found in OBx rats. Titers of the virus producing cell line might not be high enough or infection force of virus env gene would be too weak.