Immunohistochemical study on the rabbit cerebellum in early postnatal periods

本文分析了幼兔和胎兔小脑对抗成年兔嗅球的单抗11-4G12(MAb 4G12)的免疫染色模式。在出生后早期，单抗4G12在前叶强标记浦肯野细胞，而单抗在后叶弱标记浦肯野细胞。前者为周长枝晶阶段，后者为主斜闪石阶段。前叶和后叶在形态特征和免疫染色强度上有很大差异，在胚胎第25天也观察到上述区域免疫染色强度的差异，两叶中的浦肯野细胞具有不成熟的形态。因此，单抗4G12免疫反应的区域差异不是由于浦肯野细胞成熟的差异所致。这表明，兔小脑浦肯野细胞在发育过程中是通过分子异质性分离的。免疫印迹分析表明，单抗4G12的表位分子量为26kd。

Immunostaining patterns of the cerebellum toward a monoclonal antibody, 11-4G12 (MAb 4G12), produced against the adult rabbit olfactory bulb were analysed in baby rabbits and rabbit fetuses. In the early postnatal periods, MAb 4G12 labeled intensely Purkinje cells in the anterior lobe whereas the MAb labeled slightly Purkinje cells in the posterior lobe. The former belonged to a perisomatic dendrite stage whereas the latter did to a main dndrite stage. There are great differences in morphological characteristics and immunostaining intensities between the anterior and posterior lobes.The abovementioned regional differences in immunostaing intensity also were observed at embryonic day 25 in which Purkinje cells in both lobes have an immature morphology. Thus, it is concluded that the regional difference in immunoreaction to MAb 4G12 is not due to difference in Purkinje cell maturation. This suggests that Purkinje cells of the rabbit cerebellum during development are segregated by molecular heterogenuiety. From immunobloting analyses, an epitope against MAb 4G12 had estimated molecular weights of 26 kd units.