Roles of liver and intestinal fatty acid binding protein gastrointestinal tract participating in fat absorption and colorectal cancer

本研究旨在阐明以下两个问题：1. L -和I-FABP参与小肠脂肪酸吸收的功能差异。2.方法和结果如下：1.采用SRID法对5组饲喂高、低脂饲料2周和4周的大鼠十二指肠、空肠和回肠中的两种FABP进行定量测定。空肠的FABP含量高于回肠和十二指肠。高脂饲料组L-FABP仅在空肠显著升高，I-FABP在回肠和空肠显著升高。低脂饮食组与正常对照组的FABP含量无明显差异。这些结果支持了高脂肪摄入诱导的脂肪酸代谢产物的概念，并且这些脂肪酸代谢产物在脂肪酸的吸收和转运中具有不同的作用。2.应用PAP法检测82例晚期大肠癌患者L-FABP免疫染色强度。FABP免疫染色强度随结直肠癌的组织学类型、淋巴结转移和组织学分期而变化。在全组和60例根治性切除的患者中，FABP免疫染色强度高的患者的累积术后生存率显著高于低强度者（p<0.001），提示FABP免疫染色强度对晚期结直肠癌患者的预后有一定意义。

The aim of this study was to clarify following two questions, 1.the functional difference between L - and I- FABP participating in the absorption of fatty acids in the small intestine. 2.The role of L - FABP in the colorectum, as to its affinity to carcinogens.Methods and results are as follows ; 1.Quantitive measurement of the two FABP in duodenum, jejunum and ileum of five groups of rats, which were fed diets with high and low fat contents during two or four weeks, was carried out by SRID method. Jejunum showed the highest content of both FABPs than ileulm and duodenum. Only in the jejunum, L-FABP significantly increased in high fat diet group while I-FABP increased in the ileum and jujunum. Both FABPs concentration showed no difference between in low fat diet group and normal cotrols. These findings support the concept that FABPs are inducted by high fat intake, and those have different roles in fatty acid absorption and transport. 2.The intensity of L-FABP immunostaining vy PAP mothod with anti-FABP antivodies was studied in 82 patients with advanced colorectal cancer. The untensity of FABP immnunostaining varied significantly with the histologic type, node metastasis, and histologic stage of colorectal cancer. The cumulative postoperative survival rate was siginificantly higher in the patients with high intensity FABP immunostaining than in those with low intensive staining both in the entire series and in a series of 60 patients with curative resection (p<0.001).These results suggest that the intensity of FABP immunostaining might indicate a prognostic role in patients with advanced colorectal cancer.