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Roles of liver and intestinal fatty acid binding protein gastrointestinal tract participating in fat absorption and colorectal cancer

肝肠脂肪酸结合蛋白胃肠道参与脂肪吸收与结直肠癌的作用

基本信息

批准号：
04454328
负责人：
HATAKEYAMA Katsuyoshi
金额：
$ 3.26万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The aim of this study was to clarify following two questions, 1.the functional difference between L - and I- FABP participating in the absorption of fatty acids in the small intestine. 2.The role of L - FABP in the colorectum, as to its affinity to carcinogens.Methods and results are as follows ; 1.Quantitive measurement of the two FABP in duodenum, jejunum and ileum of five groups of rats, which were fed diets with high and low fat contents during two or four weeks, was carried out by SRID method. Jejunum showed the highest content of both FABPs than ileulm and duodenum. Only in the jejunum, L-FABP significantly increased in high fat diet group while I-FABP increased in the ileum and jujunum. Both FABPs concentration showed no difference between in low fat diet group and normal cotrols. These findings support the concept that FABPs are inducted by high fat intake, and those have different roles in fatty acid absorption and transport. 2.The intensity of L-FABP immunostaining vy PAP mothod with anti-FABP antivodies was studied in 82 patients with advanced colorectal cancer. The untensity of FABP immnunostaining varied significantly with the histologic type, node metastasis, and histologic stage of colorectal cancer. The cumulative postoperative survival rate was siginificantly higher in the patients with high intensity FABP immunostaining than in those with low intensive staining both in the entire series and in a series of 60 patients with curative resection (p<0.001).These results suggest that the intensity of FABP immunostaining might indicate a prognostic role in patients with advanced colorectal cancer.

本研究旨在阐明以下两个问题：1.L-FABP与I-FABP参与小肠脂肪酸吸收的功能差异。 2.L-FABP在结直肠中的作用，及其与致癌物的亲和力。方法和结果如下； 1.采用SRID法对高、低脂肪饲料喂养的5组大鼠十二指肠、空肠、回肠中两种FABP进行定量测定。空肠的两种 FABP 含量均高于回肠和十二指肠。仅在空肠中，高脂饮食组中L-FABP显着增加，而I-FABP在回肠和空肠中增加。低脂饮食组和正常对照组之间两种 FABP 浓度均无差异。这些发现支持了 FABP 是由高脂肪摄入诱导的概念，并且它们在脂肪酸吸收和运输中具有不同的作用。 2.对82例晚期结直肠癌患者进行了PAP法和抗FABP抗体的L-FABP免疫染色强度的研究。 FABP免疫染色强度随结直肠癌的组织学类型、淋巴结转移、组织学分期的不同而有显着差异。在整个系列和 60 名根治性切除患者的系列中，高强度 FABP 免疫染色患者的累积术后生存率显着高于低强度染色患者 (p<0.001)。这些结果表明 FABP 免疫染色强度可能表明晚期结直肠癌患者的预后作用。