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Role of NKT cells and induction of tolerance using α-GalCer in swine small bowel transplantation model

NKT 细胞的作用和使用 α-GalCer 诱导耐受在猪小肠移植模型中的作用

基本信息

批准号：
14370380
负责人：
HATAKEYAMA Katsuyoshi
金额：
$ 3.14万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370380/
关键词：
NKT cells Swine small intestinal transplantation Tolerance α-GalCer

项目摘要

To produce anti-swine NK receptor antibody, we immunized swine lymphcytes or membranous antigen to mice and rats. However, the antibodies could not stained swine NK cells. We sometimes tried the same experiments, but antibodies for staining swine NK cells were not produced. On the other hand, we tried swine small intestine transplantation. At the first time, the experiments were in failure, however gradually the experiments became in success. Finally, we could have long survival cases after amall intestinal transplantation. Next we injected α-Gal Cer to the small bowel recipient pigs. However bcause α-Gal Cer were injected to few transplantated pigs, we could not compare the survival period between injected group and no injected group. We can not confirmed relationship between α-Gal Cer and NKT cells in pigs, because we could not produce swine NK antibodies. However these results don't deny hypothesis that α-Gal Cer induce immunological tolerance after small intestinal transplantation. NKT cells are well recognized in mice and human. We found relationships between NKT cells and immunological tolerance in human living related donor liver transplantations. And we have experienced many murine experiments about NKT cells. It may be necessary to examine the method for administration of the drugs. To confirm induction of immunological tolerance using NKT cells and α-Gal Cer, we need further investigation in murine intestinal transplantation model.

为了制备抗猪NK受体抗体，我们用猪淋巴细胞或膜抗原免疫小鼠和大鼠。然而，抗体不能染色猪NK细胞。我们有时也尝试同样的实验，但没有产生用于染色猪NK细胞的抗体。另一方面，我们尝试了猪小肠移植。第一次实验失败了，但逐渐实验成功了。最后，我们可以有长期存活的病例。接下来，我们将α-Gal Cer注射到小肠受体猪中。但由于注射α-Gal Cer的猪只很少，因此不能比较注射组和未注射组的存活时间。由于不能产生猪NK抗体，因此不能证实α-Gal Cer与猪NKT细胞的关系。但这些结果并不否定α-Gal Cer诱导小肠移植免疫耐受的假说。NKT细胞在小鼠和人类中被充分识别。我们发现NKT细胞与活体供肝移植免疫耐受的关系。我们已经进行了许多关于NKT细胞的小鼠实验。可能需要检查药物的给药方法。NKT细胞和α-Gal Cer能否诱导免疫耐受有待于进一步的研究。