Role of NKT cells and induction of tolerance using α-GalCer in swine small bowel transplantation model

为了制备抗猪NK受体抗体，我们用猪淋巴细胞或膜抗原免疫小鼠和大鼠。但该抗体不能对猪NK细胞进行染色。我们有时会尝试同样的实验，但没有产生染色猪NK细胞的抗体。另一方面，我们尝试了猪小肠移植。起初，实验失败了，但渐渐地，实验成功了。最后，在全肠移植后，我们可以有长期存活的病例。接下来，我们将α-GalCer注射到小肠受体猪体内。但由于注射α-Gal Cer的移植猪较少，因此不能比较注射组和未注射组的存活时间。α-GalCer与猪NKT细胞之间的关系尚不能确定，因为我们不能产生猪NK抗体。然而，这些结果并不否认α-GalCer诱导小肠移植后免疫耐受的假说。NKT细胞在小鼠和人类中都得到了很好的识别。在活体亲属供肝移植中，我们发现NKT细胞与免疫耐受之间的关系。我们已经经历了许多关于NKT细胞的小鼠实验。可能有必要检查给药方法。为了证实NKT细胞和α-GalCer诱导免疫耐受，我们还需要在小鼠肠道移植模型上进行进一步的研究。

To produce anti-swine NK receptor antibody, we immunized swine lymphcytes or membranous antigen to mice and rats. However, the antibodies could not stained swine NK cells. We sometimes tried the same experiments, but antibodies for staining swine NK cells were not produced. On the other hand, we tried swine small intestine transplantation. At the first time, the experiments were in failure, however gradually the experiments became in success. Finally, we could have long survival cases after amall intestinal transplantation. Next we injected α-Gal Cer to the small bowel recipient pigs. However bcause α-Gal Cer were injected to few transplantated pigs, we could not compare the survival period between injected group and no injected group. We can not confirmed relationship between α-Gal Cer and NKT cells in pigs, because we could not produce swine NK antibodies. However these results don't deny hypothesis that α-Gal Cer induce immunological tolerance after small intestinal transplantation. NKT cells are well recognized in mice and human. We found relationships between NKT cells and immunological tolerance in human living related donor liver transplantations. And we have experienced many murine experiments about NKT cells. It may be necessary to examine the method for administration of the drugs. To confirm induction of immunological tolerance using NKT cells and α-Gal Cer, we need further investigation in murine intestinal transplantation model.