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Studies on pathophysiology after hepatectomy for dogs with obstructive jaudice under occlusion of hepatic blood inflow

肝血流阻断下梗阻性黄疸犬肝切除术后的病理生理学研究

基本信息

批准号：
04454329
负责人：
MIZUMOTO Ryuji
金额：
$ 4.42万
依托单位：
MIE University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Obstructive jaundice was produced in adult mongrel dogs by cholecystectomy and ligation and cutting of the distal common bile duct. The pathophysiology of ischemia-reperfusion injury on remnant liver after hepatectomy and the occlusion of hepatic blood inflow was evaluated in dogs with obstructive jaundice.1.When 40% hepatectomy under 10-minute occlusion of both hepatic artery and portal vein was performed for dogs with obstructive jaundice at 2 weeks after ligation of the common bile duct and chole-cystectomy, the content of lipid peroxide increased significantly in the remnant liver with reduction of SOD activity, while xanthine oxidase activity did not change. The levels of endotoxin and beta-NAH in peripheral blood had significantly increased, and phagocytic index had markedly decreased after 3 hours. Survival rate at 7 days was only 23.1% and many dogs died of liver failure.2.Effects of free radical scavengers : When Coenzyme Q_<10>(CoQ_<10>) or stirene-co-maleic acid superoxide d … More ismutase(SM-SOD) was administered before ischemia and reflow, the development of the above phenomenon in the remnant liver was prevented significantly and the survival rate at 7days was improved remarkably to 76.9% and 69.2%. The endotoxin levels in the portal venous blood were increased significantly after reflow of the hepatic blood inflow, regardless of the administration of CoQ_<10> or SM-SOD.Whereas, the levels in the peripheral blood were markedly reduced by these drugs.3.Effects of active bypass of the portal vein to inferior vena cava : When the hepatic blood inflow was occluded for 10 minutes with active bypass of the portal vein to inferior vena cava, the levels of endotoxin in both the portal venous blood and the peripheral blood did not change, and the remnant liver injury was milder. However, when the hepatic blood inflow was occuluded for 20 minutes with active bypass, the content of lipid peroxide increased significantly in the remnant liver with reduction of SOD activity, while the levels of endotoxin in both the portal venous blood and the peripheral blood remained the same. The level beta-NAH in peripheral blood increased significantly and the phagocytic index decreased markedly after 3 hours. Survival rate at 7 days was only 33.3% and many dogs died of liver failure. Less

采用胆囊切除术和胆总管远端结扎切断法，在成年杂种犬中制备梗阻性黄疸模型。本实验观察了梗阻性黄疸犬肝切除术后肝血流阻断对残肝缺血-再灌注损伤的病理生理学变化：1.梗阻性黄疸犬在胆总管和胆囊结扎后2周行40%肝切除术，阻断肝动脉和门静脉10分钟。残肝组织中脂质过氧化物含量显著增加，SOD活性降低，而黄嘌呤氧化酶活性无明显变化。3小时后外周血内毒素和β-NAH水平明显升高，吞噬指数明显下降。自由基清除剂的作用：辅酶Q_<10>（CoQ_）或苯乙烯-co-马来酸超氧化物歧化酶（SOD）对狗的自由基清除作用：辅酶Q_（CoQ_<10>）或苯乙烯-co-马来酸超氧化物歧化酶（SOD）对狗的自由基清除作用 ...更多信息在缺血再灌注前给予SM-SOD，可明显阻止残肝上述现象的发生，使7天存活率明显提高，分别为76.9%和69.2%。门静脉血内毒素水平在肝血流回流后明显升高，<10>而外周血内毒素水平则明显降低。3.门静脉主动转流对下腔静脉的影响：当肝血流阻断10分钟，门静脉主动转流至下腔静脉时，门静脉血和外周血中的内毒素水平没有变化，残余肝损伤较轻。但阻断肝血流20 min后，残肝脂质过氧化物含量明显升高，SOD活性降低，而门静脉血和外周血内毒素水平无明显变化。3 h后外周血β-NAH水平明显升高，吞噬指数明显下降。7天的存活率仅为33.3%，许多犬死于肝功能衰竭。少