权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on implantable artificial liver

植入式人工肝的研究

基本信息

批准号：
04454332
负责人：
IKEDA Yoshito
金额：
$ 4.86万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The hybrid-typed artificial liver composed of hepatocytes and polymer matrices has been increasingly notad as a new therapeutic trial of acute liver failure. The encapsulation technology of hepatocytes by polymer hydrogels will prevent the cells from allogeneically or xenogeneically immune attack, allowing their metabolic functions to perform in the recipients. However, little has been reported on the physicochemical prooerties of polymer materials themselves although they may provide an informative suggestion for cell encapsulation. The present research was undertaken to obtain basic knowledge about the hydrogel materials applicable for artificial liver. The polyion complex membrane composed of sodium alginate and poly (allyl amine) (PAA) was selected since the cell encapsulation can be achieved under mild conditions, e.g.at 37ﾟC in aqueous solution. The water content and mechanical strength of the membrane or its permeability of proeteins, albumin and immunoglobulim, were similar to those of aliginate-poly (L-lysine) polyion complex membrane which has been widely used for hepatocye encapsulation. However, a big difference in the viability of rat hepatocytes was observed before and after their encapsulation process. It was found that the viability decrease was responsible for the contact of cells with calcium ions which is a necessary process for cell encapsulation. This indicates that it is important to make the contact time as short as possible for cell encapsulation.

由肝细胞和聚合物基质组成的复合型人工肝作为治疗急性肝衰竭的一种新的尝试已日益受到关注。高分子水凝胶对肝细胞的包封技术可以防止同种异体或异种免疫攻击，使其在受体体内发挥代谢功能。然而，很少有报道聚合物材料本身的物理化学性质，虽然他们可能提供一个翔实的建议，细胞封装。本研究旨在获得适用于人工肝的水凝胶材料的基础知识。选择由海藻酸钠和聚（烯丙基胺）（PAA）组成的聚离子复合物膜，因为可以在温和的条件下（e.g.at 37 ℃）在水溶液中实现细胞包封。膜的含水量和机械强度或其对蛋白质、白蛋白和免疫球蛋白的渗透性与已广泛用于肝细胞包封的海藻酸盐-聚（L-赖氨酸）聚离子复合物膜的那些相似。然而，在其封装过程之前和之后，观察到大鼠肝细胞的活力存在很大差异。结果发现，细胞与钙离子的接触是细胞包封的必要过程，而细胞活力的降低是导致细胞与钙离子接触的原因。这表明，使接触时间尽可能短对于细胞包封是重要的。