权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Experimental Study of Spinal Deformity Produced in Dogs with Syringomyelia

犬脊髓空洞症造成脊柱畸形的实验研究

基本信息

批准号：
04454370
负责人：
KITAHARA Hiroshi
金额：
$ 4.35万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The purpose of this study is to investigate the etiology of scoliosis produced in dogs with kaolin-induced syringomyelia.Kaolin was injected into the cisterna magna of 9 beagles from 6 to 8 weeks after birth. To evaluate bony growth by staining, calcein and tetracycline subcutaneously administered during the growth period. The animals were sacrificed 8 weeks (2dogs), 10(1), 14(2), 18(1), 26(2), 52(1) after injection of kaolin. Roentgenograms, CT scans, and MRI were obtained. The spinal cord was histologically examined, and the structural changes of the vertebral column were analyzed histomorphometrically.Hydrocephalus was noted in 7 dogs. A comunicating-type syringomyelia was appeared in 4 dogs in association with hydrocephalus. The histological study of the spinal cord revealed acute or subacute inflammatory changes, including stretching and thinning of the ependymal cells, formation of periependymal edema, and spongy changes within the periependymal tissues. Mild scoliosis were recognized in 2 dogs at 10 and 14 weeks, and a significant scoliosis with main curvature of 66ﾟ was noted in 1 dog at 14 weeks after kaolin injection. In a dog with significant scoliosis, abnormal spinal modeling, suggesting that the bone growth was attempting to correct rather than produce deformity, was shown histomorphometrically.It has been shown that kaolin injection into the cisterna magna produced basilar arachnoiditis leading to hydrocephalus and syringomyelia. However, there is no report on the scoliotic deformity associated with kaolin-induced syringomyelia.The paravertebral muscle of scoliotic deformity will be histologically examined in future.

本研究的目的是探讨高岭土诱导脊髓空洞犬脊柱侧凸的病因。9只小猎犬出生后6 ~ 8周注射高岭土至大池。通过骨染色、钙黄蛋白和四环素的皮下注射评估骨生长情况。分别于注射高岭土后8周（2只）、10周（1只）、14周（2只）、18周（1只）、26周（2只）、52周（1只）处死。获得x线片、CT扫描和MRI。对脊髓进行组织学检查，并对脊柱的结构变化进行组织形态学分析。7只狗出现脑积水。4只犬伴脑积水出现交流型脊髓空洞。脊髓的组织学检查显示急性或亚急性炎症改变，包括室管膜细胞的拉伸和变薄，室管膜周围水肿的形成，室管膜周围组织的海绵状改变。2只狗在注射高岭土后10周和14周出现轻度脊柱侧凸，1只狗在注射高岭土后14周出现明显的脊柱侧凸，主弧度为66瓣。在患有严重脊柱侧凸的狗中，异常的脊柱模型显示，骨骼生长试图纠正而不是产生畸形。研究表明，大池注射高岭土可引起基底蛛网膜炎，导致脑积水和脊髓空洞。然而，尚无高岭土诱导的脊髓空洞引起脊柱侧凸畸形的报道。脊柱侧弯畸形的椎旁肌将在未来进行组织学检查。