Radiological and histological analyzes of the structural changes in the rat parotid gland following release of Stensen's duct obstruction

Stensen 导管阻塞解除后大鼠腮腺结构变化的放射学和组织学分析

• 批准号: 04454505
• 负责人: KANDA Shigenobu
• 金额: $ 4.42万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454505/
• 关键词: Rat; Paratid giand; Sialography; Obstruction; Recovery; 結紮

Sialography is the preferred method for diagnosis and treatment planning in obstructive sialoadenitis. Many experimental studies reported the changes following duct obstruction. However, sialographic changes following release of obstruction have not been studied. The present study was undertaken to clarify ; (1)the relationship between sialographic and histological changes, (2)the effects of duration and pressure of obstruction on gland recovery, (3)the possibility to establish sialographic features indicating the prognosis for gland recovery. Stensen's ducts of rat parotid glands were obstructed over 2 time courses under 3 varying pressures in order to induce variant degrees of degenerative changes on glandular tissue. The structural alteration resulting from release of obstruction was investigated by means of qualitative and quantitative microsialography and histology. Gland structure was assessed visually from the latter and classified into 6 groups ; normal, recovery and slight, moderate and severe degeneration and fibrous groups. Microsialograms were evaluated based on these histological groups. Qualitatively, microsialograms of the operated glands showed stricture at the obstructed region and dilation of the major ducts. Hsitological defferences between recovery and degeneration glands were reflected on the figures of peripheral ducts. The former showed a branch-like morphology similar to that of the normal group, and the latter showed small granule-like figures. Quantitatively, dilation of major ducts in the recovery group decreased compared with the other operated groups. With regard to degeneration and fibrous groups, there were no distinct sialographic features which correlated with the degree of degeneration observed histologically. Duration and pressure of obstruction were highly predictive of prognosis for recovery of the glands. At the time of release of obstruction, however, it seems difficult to determine the prognosis for gland recovery by sialogra

涎腺造影是诊断和治疗阻塞性涎腺炎的首选方法。许多实验研究报道了胆管阻塞后的变化。然而，还没有研究过阻塞解除后的涎管造影变化。本研究旨在阐明：（1）涎腺造影与组织学变化之间的关系，（2）阻塞时间和压力对腺体恢复的影响，（3）建立涎腺造影特征指示腺体恢复预后的可能性。在3种不同压力下，阻塞大鼠腮腺Stensen管2个时间过程，以引起腺体组织不同程度的退行性改变。通过定性和定量的显微涎腺造影和组织学研究了阻塞解除所引起的结构变化。对后者的腺体结构进行目视评估，并分为6组：正常、恢复和轻度、中度和重度变性和纤维组。根据这些组织学分组评价了显微涎腺造影。手术腺体的定性、显微涎腺造影显示阻塞区域狭窄和主要导管扩张。恢复期腺体与退化期腺体的组织学差异反映在外周导管的形态上。前者呈树枝状，后者呈小颗粒状。术后恢复组主动脉扩张程度较其他手术组明显减轻。关于变性和纤维组，没有明显的与组织学观察到的变性程度相关的涎管造影特征。阻塞的持续时间和压力是高度预测腺体恢复的预后。然而，在梗阻解除时，似乎难以确定唾液腺恢复的预后。